Line 138: | Line 138: | ||
<h3><font size="6">Method</font></h3> | <h3><font size="6">Method</font></h3> | ||
− | < | + | <br><br> |
+ | <p style="font-family: roboto;font-size:150%;">We follow exactly the interlab protocol provided by iGEM(link) </p> | ||
+ | |||
+ | <br> | ||
+ | <div class="some-padding"></div> | ||
+ | <div class="some-padding"></div> | ||
+ | <div class="some-padding"></div> | ||
+ | <div class="some-padding"></div> | ||
+ | <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true"> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="P0"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P0-collapse" aria-expanded="false" aria-controls="P0-collapse"> | ||
+ | <div> | ||
+ | <div class="col-md-11">Plate reader setting </div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div> | ||
+ | </div> | ||
+ | </a> | ||
+ | </h4> | ||
+ | |||
+ | </div> | ||
+ | <div id="P0-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P0"> | ||
+ | <div class="panel-body"> | ||
+ | <ol> | ||
<table width="80%"> | <table width="80%"> | ||
<tr> | <tr> | ||
− | <th colspan="2">Microplate used: Corning® clear flat bottom black 96 well plates</th> | + | <th colspan="2" >Microplate used: Corning® clear flat bottom black 96 well plates</th> |
</tr> | </tr> | ||
Line 159: | Line 181: | ||
<tr> | <tr> | ||
<th>No. of flashes per well</th> | <th>No. of flashes per well</th> | ||
− | <th>10</th> | + | <th bgcolor="#FFFFFF">10</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<th>Scan mode</th> | <th>Scan mode</th> | ||
− | <th>orbital averaging</th> | + | <th bgcolor="#FFFFFF">orbital averaging</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<th>Scan diameter [mm]</th> | <th>Scan diameter [mm]</th> | ||
− | <th>3</th> | + | <th bgcolor="#FFFFFF">3</th> |
</tr> | </tr> | ||
Line 178: | Line 200: | ||
<tr> | <tr> | ||
<th>Excitation</th> | <th>Excitation</th> | ||
− | <th>600</th> | + | <th bgcolor="#FFFFFF">600</th> |
</tr> | </tr> | ||
Line 191: | Line 213: | ||
<tr> | <tr> | ||
<th>Settling time [s]</th> | <th>Settling time [s]</th> | ||
− | <th>0.1</th> | + | <th bgcolor="#FFFFFF">0.1</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<th>Reading direction</th> | <th>Reading direction</th> | ||
− | <th>bidirectional, horizontal left to right, top to bottom</th> | + | <th bgcolor="#FFFFFF">bidirectional, horizontal left to right, top to bottom</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<th>Target temperature [°C]</th> | <th>Target temperature [°C]</th> | ||
− | <th>25</th> | + | <th bgcolor="#FFFFFF">25</th> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<th>No. of flashes per well</th> | <th>No. of flashes per well</th> | ||
− | <th>8</th> | + | <th bgcolor="#FFFFFF">8</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<th>Scan mode</th> | <th>Scan mode</th> | ||
− | <th>orbital averaging</th> | + | <th bgcolor="#FFFFFF">orbital averaging</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<th>Scan diameter [mm]</th> | <th>Scan diameter [mm]</th> | ||
− | <th>3</th> | + | <th bgcolor="#FFFFFF">3</th> |
</tr> | </tr> | ||
Line 233: | Line 255: | ||
<tr> | <tr> | ||
<th>Excitation:</th> | <th>Excitation:</th> | ||
− | <th>470-15</th> | + | <th bgcolor="#FFFFFF">470-15</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<th>Emission</th> | <th>Emission</th> | ||
− | <th>515-20</th> | + | <th bgcolor="#FFFFFF">515-20</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<th>Gain</th> | <th>Gain</th> | ||
− | <th>500</th> | + | <th bgcolor="#FFFFFF">500</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<th>Focal height [mm]</th> | <th>Focal height [mm]</th> | ||
− | <th>9</th> | + | <th bgcolor="#FFFFFF">9</th> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<th>Reading direction</th> | <th>Reading direction</th> | ||
− | <th>bidirectional, horizontal left to right, top to bottom</th> | + | <th bgcolor="#FFFFFF">bidirectional, horizontal left to right, top to bottom</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<th>Target temperature [°C]</th> | <th>Target temperature [°C]</th> | ||
− | <th>25 | + | <th bgcolor="#FFFFFF">25 |
</th> | </th> | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | |||
+ | </ol> | ||
+ | |||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
Revision as of 15:17, 2 October 2017