Difference between revisions of "Team:KUAS Korea/Design"
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<img src="https://static.igem.org/mediawiki/2017/a/ae/Heme_final.png"> | <img src="https://static.igem.org/mediawiki/2017/a/ae/Heme_final.png"> | ||
| − | + | <br> | |
<h4>1. Prepare catholyte and anolyte</h4> | <h4>1. Prepare catholyte and anolyte</h4> | ||
<p><font size=4>The hssR and hssS mechanism of our project is as follows.</font></p> | <p><font size=4>The hssR and hssS mechanism of our project is as follows.</font></p> | ||
| − | <p> <font size=4> ① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br> | + | <p><font size=4> ① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br> |
② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.<br> | ② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.<br> | ||
③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.<br> | ③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.<br> | ||
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</font><br> | </font><br> | ||
</p> | </p> | ||
| − | + | <br> | |
<h4>2. Prepare catholyte and anolyte</h4><br> | <h4>2. Prepare catholyte and anolyte</h4><br> | ||
<p><font size=4>Also, the mechanism of heme detection using hrtR protein is as follows.</font></p> | <p><font size=4>Also, the mechanism of heme detection using hrtR protein is as follows.</font></p> | ||
| − | <p> <font size=4> ①If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br> | + | <p><font size=4> ①If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br> |
② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.<br> | ② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.<br> | ||
③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.<br> | ③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.<br> | ||
Revision as of 12:01, 7 October 2017
Design
POO-robiotics
1. Prepare catholyte and anolyte
The hssR and hssS mechanism of our project is as follows.
① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.
③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.
④ The phosphorylated HssR binds to the direct repeat sequence of the hrtAB promoter and initiates the reporter’s transcription.
2. Prepare catholyte and anolyte
Also, the mechanism of heme detection using hrtR protein is as follows.
①If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.
③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.
