Difference between revisions of "Team:Lethbridge HS/Demonstrate"
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<h2>Ink Production</h2> | <h2>Ink Production</h2> | ||
| − | <p class="center">Our final product from the cells will be the pigments we require to make ink, but before we can use them we must extract them from the cells> The way we will be extracting the pigment is via organic extraction. We will first | + | <p class="center">Our final product from the cells will be the pigments we require to make ink, but before we can use them we must extract them from the cells> The way we will be extracting the pigment is via organic extraction. We will first centrifuge the cells and liquid LB broth media in order to get just the cells and pigment in the pellet. This will allow us to pour off the supernatant which will be the media to get the cells to work with. We will then add the solvent for the organic extraction and depending on the pigment it will be either acetone or methanol. This will lyse the cells as well as have our pigment dissolved in it. We will then homogenize the mixture in order to destroy all of the rest of the cell membranes and any living cells. THis homogenized solution will then be put through a series of filters in order to get out the larger proteins and all of the parts of the cells, this will leave us with pigment and the solvent and all that is left is the evaporation of the solvent. |
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<center><img src="https://static.igem.org/mediawiki/2017/f/fa/T--Lethbridge_HS--Extraction.png"> | <center><img src="https://static.igem.org/mediawiki/2017/f/fa/T--Lethbridge_HS--Extraction.png"> | ||
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| − | This image shows the process of our pigment extraction from the cells starting with live cells with pigment inside of them and ending with our pigment. | + | Fig. 1 <i>This image shows the process of our pigment extraction from the cells starting with live cells with pigment inside of them and ending with our pigment.</i> |
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Revision as of 15:40, 10 October 2017
Ink Production
Our final product from the cells will be the pigments we require to make ink, but before we can use them we must extract them from the cells> The way we will be extracting the pigment is via organic extraction. We will first centrifuge the cells and liquid LB broth media in order to get just the cells and pigment in the pellet. This will allow us to pour off the supernatant which will be the media to get the cells to work with. We will then add the solvent for the organic extraction and depending on the pigment it will be either acetone or methanol. This will lyse the cells as well as have our pigment dissolved in it. We will then homogenize the mixture in order to destroy all of the rest of the cell membranes and any living cells. THis homogenized solution will then be put through a series of filters in order to get out the larger proteins and all of the parts of the cells, this will leave us with pigment and the solvent and all that is left is the evaporation of the solvent.
Fig. 1 This image shows the process of our pigment extraction from the cells starting with live cells with pigment inside of them and ending with our pigment.
