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+ | <div class="navbar-nav"> | ||
+ | <ul id="nav-list-ul" class="nav-list"> | ||
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+ | <a href="#">TEAM</a> | ||
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+ | <a href="#">PRACTICE</a> | ||
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+ | <li><a href="https://2017.igem.org/Team:SZU-China/Engagement">ENGAGEMENT</a></li> | ||
+ | </ul> | ||
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+ | <a href="#">EXPERIMENT</a> | ||
+ | <span class="caret"></span> | ||
+ | <ul class="sub-nav" style="top:-5px"> | ||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Procedure">PROCEDURE</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Results">RESULTS</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Demonstrate">DEMONSTRATE</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Protocol">PROTOCOL</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Notebook">NOTEBOOK</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="#">PROJECT</a> | ||
+ | <span class="caret"></span> | ||
+ | <ul class="sub-nav" style="top:-5px"> | ||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Description">DESCRIPTION</a></li> | ||
+ | |||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Design">DESIGN</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Model">MODEL</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Safety">SAFTY</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li> | ||
+ | <a href="#">ACHIEVEMENT</a> | ||
+ | <span class="caret"></span> | ||
+ | <ul class="sub-nav" style="top:-5px"> | ||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Achievement">MEDAL</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:SZU-China/Parts">PARTS</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div> | ||
+ | <a href="https://2017.igem.org/Team:SZU-China"><img src="https://static.igem.org/mediawiki/2017/1/17/T--SZU-China--team-logo.png" style="height: 36px;width: auto;padding-top: 12px;padding-left:10px;"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div style="position: relative;top: 40px;"> | ||
+ | |||
+ | <section id="overview" style="padding:106px 0 80px 0;background-color:white;"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-sm-12"> | ||
+ | <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:50px"><center>PARTS</center> </h3> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
+ | |||
+ | |||
+ | <p></p><br /><br /> | ||
+ | <section id="overview" class="cd-section" style="padding: 66px 0 50px 0; background-color: rgba(245,245,245,0.45);"> | ||
+ | <div style="position: relative;margin: 30px 14%;"> | ||
+ | |||
+ | <p></p> | ||
+ | <div class="col-sm-12"> | ||
+ | <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:28px;font-weight:500"><center>Basic Parts</center> </h3> | ||
+ | <span class="font1">This year, our team ultilizes 2 kinds of new parts: one is for carbonic anhydrase expression, another is for Na<sup>+</sup>-H<sup>+</sup> antiporter, which can help improve alkaline resistance.You can click on the part number to see the details.</span><br /><br /> | ||
+ | <br/> | ||
+ | </div> | ||
+ | <p></p> | ||
+ | <table class="table" style="width:75%;margin:0 auto"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <th style="width:120px">Part Number</th> | ||
+ | <th style="width:150px">Name</th> | ||
+ | <th style="width:80px">Type</th> | ||
+ | |||
+ | <th style="width:300px">Part Description</th> | ||
+ | |||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232000">BBa_K2232000</a></td> | ||
+ | <td>TSLV1-CA-α</td> | ||
+ | <td>Basic</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Coding sequence for Carbonic Anhydrase α from<i> Bacillus Subtilus TSLV1 </i></td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232005">BBa_K2232005</a></td> | ||
+ | <td>OF4-CA-α</td> | ||
+ | <td>Basic</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Coding sequence for Carbonic Anhydrase α from<i> Bacillus Subtilus OF4 </i></td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232001">BBa_K2232001</a></td> | ||
+ | <td>OF4-nhaC</td> | ||
+ | <td>Basic</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus OF4 </i></td> | ||
+ | |||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232002">BBa_K2232002</a></td> | ||
+ | <td>c125-nhaC</td> | ||
+ | <td>Basic</td> | ||
+ | |||
+ | <td>Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus c125</i></td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232007">BBa_K2232007</a></td> | ||
+ | <td>BS168-nhaC</td> | ||
+ | <td>Basic</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus 168 </i></td> | ||
+ | |||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232003">BBa_K2232003</a></td> | ||
+ | <td>c125-TupA</td> | ||
+ | <td>Basic</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Coding sequence for another Na<sup>+</sup> - H<sup>+</sup> antiporter TupA from<i> Bacillus Subtilus c125 </i></td> | ||
+ | |||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | <br /><br /> | ||
+ | <p></p><p></p> | ||
+ | <span class="font1"></span> | ||
+ | <span></span><br /> | ||
+ | <span></span><br /> | ||
+ | <span></span><br /> | ||
+ | </div> | ||
+ | |||
+ | <div style="position: relative;margin: 30px 14%;"> | ||
+ | |||
+ | <p></p> | ||
+ | <div class="col-sm-12"> | ||
+ | <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:28px;font-weight:500"><center>Composite Parts</center> </h3> | ||
+ | <span class="font1">The 2 kinds of basic parts described above is assembled together with promoter, ribosome binding site or terminator, which are presented in the following table. You can click on the part number to see the details.</span><br /><br /> | ||
+ | |||
+ | </div> | ||
+ | <p></p> | ||
+ | <br /> | ||
+ | <table class="table" style="width:75%;margin:0 auto"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <th style="width:120px">Part Number</th> | ||
+ | <th style="width:150px">Name</th> | ||
+ | <th style="width:80px">Type</th> | ||
+ | |||
+ | <th style="width:300px;padding-left:5px">Part Description</th> | ||
+ | |||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232011">BBa_K2232011</a></td> | ||
+ | <td>RBS-c125-TupA-T1</td> | ||
+ | <td>Composite</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Native sequence from <i> Mycobacterium NBB4 </i>, contained within the ethylene metabolism operon</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232012">BBa_K2232012</a></td> | ||
+ | <td>P43-OF4-nhaC-T1</td> | ||
+ | <td>Composite</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Coding sequence of EtnR1 from <i>Mycobacterium NBB4 </i>, codon-optimised for expression in <i> E. coli</i> K12</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232013">BBa_K2232013</a></td> | ||
+ | <td>p43-nhaC-T1</td> | ||
+ | <td>Composite</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Coding sequence of EtnR1 from <i>Mycobacterium </i> NBB4, codon-optimised for expression in<i> E. coli </i>K12</td> | ||
+ | |||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232014">BBa_K2232014</a></td> | ||
+ | <td>p43-TSLV1-CA-α</td> | ||
+ | <td>Composite</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Coding region of the pink mutant version of amilCP</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232015">BBa_K2232015</a></td> | ||
+ | <td>P43-OF4-CA-α</td> | ||
+ | <td>Composite</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Coding region of the purple mutant version of amilCP</td> | ||
+ | |||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2232026">BBa_K2232026</a></td> | ||
+ | <td>p43-CA-β</td> | ||
+ | <td>Composite</td> | ||
+ | |||
+ | <td style="width:300px;padding-left:5px">Coding region of the green mutant version of amilCP</td> | ||
+ | |||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <br /><br /> | ||
+ | <p></p><p></p> | ||
+ | <span class="font1"></span> | ||
+ | <span></span><br /> | ||
+ | <span></span><br /> | ||
+ | <span></span><br /> | ||
+ | </div> | ||
+ | <div style="position: relative;margin: 30px 14%;"> | ||
+ | <div class="col-sm-12"> | ||
+ | <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:28px;font-weight:500"><center>Improved Parts</center> </h3> | ||
+ | |||
+ | </div> | ||
+ | <span class="font1">This year, our team have improved the existing parts by either chracterization or optimize their sequence. The improved parts (in black) and original existing parts (<span style="color:#808080">in grey</span>) are presented below. You can click on the part number to see the details.</span><br /><br /> | ||
+ | |||
+ | <p></p><br/> | ||
+ | <table class="table" style="width:75%;margin:0 auto"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <th style="width:120px;">Part Number</th> | ||
+ | <th style="width:120px">Name</th> | ||
+ | <th style="width:85px">Type</th> | ||
+ | |||
+ | <th style="width:200px">Part Description</th> | ||
+ | <th style="width:150px">Improvement</th> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232020">BBa_K2232020</a><br /><a href="http://parts.igem.org/wiki/index.php/Part:BBa_K911008" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K911008</a></td> | ||
+ | <td>PsspB-gerAa</td> | ||
+ | <td>Composite</td> | ||
+ | |||
+ | <td style="padding-left:5px">Germination-promoting receptor (gerA) expression system</td> | ||
+ | <td>Optimize its sequence</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232022">BBa_K2232022</a><br /><a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1465205" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K2465205</a></td> | ||
+ | <td>p43-csos3</td> | ||
+ | <td>Composite</td> | ||
+ | |||
+ | <td style="padding-left:5px">Carbonic anhydrase (csoS3) expression system</td> | ||
+ | <td>Optimize its sequence</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232025">BBa_K2232025</a><br /><a href="http://parts.igem.org/Part:BBa_K302035" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K302035</a></td> | ||
+ | <td>mazEF</td> | ||
+ | <td>Composite</td> | ||
+ | |||
+ | <td style="padding-left:5px">Coding region of Sucrose-limitation induced kill switch</td> | ||
+ | <td>Characterize in <i>Bacillus Subtilus</i></td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | |||
+ | </table> | ||
+ | |||
+ | </div> | ||
+ | </section> | ||
</div> | </div> | ||
+ | |||
+ | <div class="arrow-div"> | ||
+ | <a id="top" href="#" title="Back to top"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/f6/T--SZU-China--arrow.png" class="arrow-img"> | ||
+ | </a> | ||
+ | </div> | ||
+ | |||
+ | </body> | ||
+ | |||
+ | |||
+ | |||
</html> | </html> |
Latest revision as of 03:08, 2 November 2017
PARTS
Basic Parts
This year, our team ultilizes 2 kinds of new parts: one is for carbonic anhydrase expression, another is for Na+-H+ antiporter, which can help improve alkaline resistance.You can click on the part number to see the details.Part Number | Name | Type | Part Description |
---|---|---|---|
BBa_K2232000 | TSLV1-CA-α | Basic | Coding sequence for Carbonic Anhydrase α from Bacillus Subtilus TSLV1 |
BBa_K2232005 | OF4-CA-α | Basic | Coding sequence for Carbonic Anhydrase α from Bacillus Subtilus OF4 |
BBa_K2232001 | OF4-nhaC | Basic | Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus OF4 |
BBa_K2232002 | c125-nhaC | Basic | Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus c125 |
BBa_K2232007 | BS168-nhaC | Basic | Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus 168 |
BBa_K2232003 | c125-TupA | Basic | Coding sequence for another Na+ - H+ antiporter TupA from Bacillus Subtilus c125 |
Composite Parts
The 2 kinds of basic parts described above is assembled together with promoter, ribosome binding site or terminator, which are presented in the following table. You can click on the part number to see the details.Part Number | Name | Type | Part Description |
---|---|---|---|
BBa_K2232011 | RBS-c125-TupA-T1 | Composite | Native sequence from Mycobacterium NBB4 , contained within the ethylene metabolism operon |
BBa_K2232012 | P43-OF4-nhaC-T1 | Composite | Coding sequence of EtnR1 from Mycobacterium NBB4 , codon-optimised for expression in E. coli K12 |
BBa_K2232013 | p43-nhaC-T1 | Composite | Coding sequence of EtnR1 from Mycobacterium NBB4, codon-optimised for expression in E. coli K12 |
BBa_K2232014 | p43-TSLV1-CA-α | Composite | Coding region of the pink mutant version of amilCP |
BBa_K2232015 | P43-OF4-CA-α | Composite | Coding region of the purple mutant version of amilCP |
BBa_K2232026 | p43-CA-β | Composite | Coding region of the green mutant version of amilCP |
Improved Parts
Part Number | Name | Type | Part Description | Improvement |
---|---|---|---|---|
BBa_K2232020 BBa_K911008 |
PsspB-gerAa | Composite | Germination-promoting receptor (gerA) expression system | Optimize its sequence |
BBa_K2232022 BBa_K2465205 |
p43-csos3 | Composite | Carbonic anhydrase (csoS3) expression system | Optimize its sequence |
BBa_K2232025 BBa_K302035 |
mazEF | Composite | Coding region of Sucrose-limitation induced kill switch | Characterize in Bacillus Subtilus |