Difference between revisions of "Team:Newcastle/Results"

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<!--- How was this stage designed? What was its sequence (if it's a part)? Was it modelled? What considerations were taken into account when making it? What challenges were there? How does the design link back to the rationale? (i.e. why does this design work). --->
 
<!--- How was this stage designed? What was its sequence (if it's a part)? Was it modelled? What considerations were taken into account when making it? What challenges were there? How does the design link back to the rationale? (i.e. why does this design work). --->
  
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<p class="CHR"> All three selected chromoproteins were made and submitted to the iGEM registry by the Uppsala 2013 team. </p>
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<p class="CHR"> They were chosen as variants to the sfGFP present in the Sensynova platform as they exhibit of strong colour readily observed in both LB cultures and in agar plates when expressed. </p>
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<p class="CHR"> All three proteins have significant sequence homologies with proteins in the GFP family. </p>
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<h8> BBa_K1033929 – aeBlue
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<p class="CHR"> The aeBlue protein is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equine. It was first extracted and characterized by Shkrob et al. 2005 under the name aeCP597 and codon optimised for E coli by Bioneer Corp. The protein has an absorption maximum at 597nm and a deep blue colour visible to the naked eye. The protein aeBlue has significant sequence homologies with proteins in the GFP family. The coding sequence for this protein was originally submitted to the registry as BBa_K1033916 by the 2012 Uppsala iGEM team. </p>
  
  

Revision as of 12:34, 13 October 2017

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Our Experimental Results

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Biochemcial Adaptor Modules: The Results

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Sarcosine Oxidase (Glyphosate to Formaldehyde)

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

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Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References
Detector Modules: The Results

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Synthetic Promoter Library

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

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Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References
Arsenic Biosensor

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

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Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References
Psicose Biosensor (Paris-Every Collaboration)

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

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Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References
Processor Modules: The Results

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Fim Standby Switch

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

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Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References
Signal Tuners

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

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Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References
Reporter Modules: The Results

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deGFP

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

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Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References
Chromoproteins

BioBricks used: BBa_K2205016 (New),BBa_K2205017 (New),BBa_K2205018 (New), BBa_K1033915 (Uppsala 2013), BBa_K1033925 (Uppsala 2013), BBa_K1033929 (Uppsala 2013)

Diagrammatic Overview: This is a caption. This is a caption. This is a caption. This is a caption. This is a caption. This is a caption.

Rationale and Aim

The Sensynova multicellular biosensor platform has been developed to overcome the limitations identified by our team [hyperlink to human practices] that hamper the success in biosensors development. One of these limits regards the lack of modularity and reusability of the various components. Our platform design, based on the expression of three main modules (Detector, Processor and Output) by three E.coli strains in co-culture, allows the switch of possible variances for each module and the production of multiple customised biosensors.

[Insert image of modules here]

This section of the project is based on testing the modularity of the system by replacing the sfGFP output part of the Sensynova platform design with three different output chromoprotein variants; BBa_K1033929 (aeBlue), BBa_K1033925 (spisPink) and BBa_K1033915 (amajLime).


Background Information
Design Stage

All three selected chromoproteins were made and submitted to the iGEM registry by the Uppsala 2013 team.

They were chosen as variants to the sfGFP present in the Sensynova platform as they exhibit of strong colour readily observed in both LB cultures and in agar plates when expressed.

All three proteins have significant sequence homologies with proteins in the GFP family.

BBa_K1033929 – aeBlue

The aeBlue protein is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equine. It was first extracted and characterized by Shkrob et al. 2005 under the name aeCP597 and codon optimised for E coli by Bioneer Corp. The protein has an absorption maximum at 597nm and a deep blue colour visible to the naked eye. The protein aeBlue has significant sequence homologies with proteins in the GFP family. The coding sequence for this protein was originally submitted to the registry as BBa_K1033916 by the 2012 Uppsala iGEM team.


Implementation
Characterisation
Conclusions and Future Work
References
Sensynova Framework Testing (IPTG Sensor): The Results

Click the headings to see experiments and results

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

Diagrammatic Overview: This is a caption. This is a caption. This is a caption. This is a caption. This is a caption. This is a caption.

Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References