Difference between revisions of "Team:Cardiff Wales/basicparts"

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<p align="center" style="background-color:#ffffff;" > <br><br>Our team used several basic parts to create our gene constructs. Here is an overview  of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.<br><br><br> </p>
 
<p align="center" style="background-color:#ffffff;" > <br><br>Our team used several basic parts to create our gene constructs. Here is an overview  of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.<br><br><br> </p>
  
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  luciferin, which can be used to quantify gene expression </td>
 
  luciferin, which can be used to quantify gene expression </td>
 
<td>1653bp</td>
 
<td>1653bp</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2404007">BBa_K2404007</a></td>
 
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    <td>PR2-PDF1.2</td>
 
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    <td>pSB1C3</td>
 
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    <td> A composite promoter of both the salicylic acid responsive PR2 at the most 5' end, and of the jasmonic acid responsive PDF1.2 at the most 3' end</td>
 
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<td>~length</td>
 
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      <td><a href="http://parts.igem.org/Part:BBa_K2404008">BBa_K2404008</a></td>
 
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    <td>PR2-GST6</td>
 
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    <td>pSB1C3</td>
 
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    <td>A composite promoter of both the salicylic acid responsive PR2 at the most 5' end, and of the salicylic acid responsive GST6 at the most 3' end</td>
 
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<td>~length</td>
 
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    <td><a href="http://parts.igem.org/Part:BBa_K2404009">BBa_K2404009</a></td>
 
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    <td>PDF1.2-GST6</td>
 
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    <td>pSB1C3</td>
 
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    <td>A composite promoter of both the jasmonic acid responsive PDF1.2 at the most 5' end, and of the salicylic acid responsive GST6 at the most 3' end</td>
 
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Revision as of 18:29, 13 October 2017




Basic Parts






Our team used several basic parts to create our gene constructs. Here is an overview of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.




Registry link Part Plasmid Summary Approximate Length
BBa_K2404000 TSH pSB1C3 A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does not have His-tags for purification 780bp
BBa_K2404001 TSHH pSB1C3 A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does have His-tags for purification 832bp
BBa_K2404002 PDF1.2 pSB1C3 An inducible promoter that responds to the presence of jasmonic acid in the cell 522bp
BBa_K2404003 PR2 pSB1C3 An inducible promoter that responds to the presence of salicylic acid in the cell 519bp
BBa_K2404004 GST6 pSB1C3 An inducible promoter that responds to the presence of salicylic acid in the cell 519bp
BBa_K2404005 WRKY30 pSB1C3 An inducible promoter that responds to the presence of cellulose-derived oligomers 507bp
BBa_K2404006 Luc+ pSB1C3 A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression 1653bp
BBa_P10401 NosT pSB1C3 A terminator from the nopaline synthase gene which is native to the Agrobacterium tumor-inducing plasmid pTiT37 285bp
BBa_P10000 LexA pSB1C3 An estradiol inducible promoter from the LexA gene which is native to Escherichia coli 362bp
BBa_P10003 35S-OTMV pSB1C3 A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus 1446bp