Solution

We are working at a diagnose tool that combines the power of high sensible methods with the affordability needed for a wide application field. Our project, named CascAID, utilizes the CRISPR/Cas effector Cas13a to quickly and reliably test for different pathogens based on their RNA. By cleaverly designing a short RNA sequence, it is possible to guide Cas13a to only cleave RNA molecules belonging to a pathogen. Once the target has been digested, Cas13a becomes active and cleaves indiscriminately other RNA molecules. If the sample contained pathogen, then digested RNA will be found, where as a negative sample won't produce digestion fragments.

To provide an affordable product, CascAID will be implemented on paper-strips, using deep-freeze technologie that allows proteins and other organic molecules to remain active over long periods of time, even at room temperature. Thanks to the cheapness of paper as a material, CascAID-strips can be single use, thus reducing the risk of cross-contamination without increasing the costs of manufacture.