Difference between revisions of "Team:Tianjin/Design"

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<h1>Design</h1>
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
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                <h1>Description</h1>
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                    <p>Description
  
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Saccharomyces cerevisiae is a single-celled organism with three types, called a, α, and a/α. In Saccharomyces cerevisiae, three cell types differ from each other in their DNA content at the MAT locus which specifies the cell types. In nature, the two haploid cell types (a and α) of this kind of budding yeast are able to interconvert in a reversible manner by DNA-rearrangement with a DSB at the MAT locus, and this process is called mating-type switching.
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This page is different to the "Applied Design Award" page. Please see the <a href="https://2017.igem.org/Team:Tianjin/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
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<p>This year, we are planning to utilize the natural phenomenon of mating-type switching to create a new concept called mating switcher for functional transformation and safeguards in Saccharomyces cerevisiae with gene-editing technique’s help. We will take this new kind of switcher into some very interesting applications, including heavy metal treatment and cell signal swiching, to improve the maneuverability of this yeast. Moreover, we will discuss the possibility of this concept’s utilization in other eukarya.</p>
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<h5>What should this page contain?</h5>
 
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<li>Explanation of the engineering principles your team used in your design</li>
 
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<li>Discussion of the design iterations your team went through</li>
 
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<li>Experimental plan to test your designs</li>
 
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<h5>Inspiration</h5>
 
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<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
 
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<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
 
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<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
 
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Revision as of 08:13, 18 August 2017

Description

Description Saccharomyces cerevisiae is a single-celled organism with three types, called a, α, and a/α. In Saccharomyces cerevisiae, three cell types differ from each other in their DNA content at the MAT locus which specifies the cell types. In nature, the two haploid cell types (a and α) of this kind of budding yeast are able to interconvert in a reversible manner by DNA-rearrangement with a DSB at the MAT locus, and this process is called mating-type switching.

This year, we are planning to utilize the natural phenomenon of mating-type switching to create a new concept called mating switcher for functional transformation and safeguards in Saccharomyces cerevisiae with gene-editing technique’s help. We will take this new kind of switcher into some very interesting applications, including heavy metal treatment and cell signal swiching, to improve the maneuverability of this yeast. Moreover, we will discuss the possibility of this concept’s utilization in other eukarya.