Difference between revisions of "Team:Lethbridge HS/Composite Part"

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<br>  
 
<br>  
 
<div class="container-fluid" style="margin-left:10%; margin-right:10%;">  
 
<div class="container-fluid" style="margin-left:10%; margin-right:10%;">  
−
<h2>&nbsp;Anthocyanin Construct</h2>
+
<h2>&nbsp;Anthocyanin Constructs</h2>
−
<p class="center"> This construct is being used to convert our initial molecule Eriodictyol into our final molecule Anthocyanin. The genes are:
+
<br>
 +
<br>
 +
<h3>Anthocyanin composite part 1</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281101</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<br>
 
<br>
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 +
<br>
 +
<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>
 
<br>
 
<br>
 
<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>
 
<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>
 
<br>
 
<br>
−
<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>
+
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
</p>
 +
<br>
 +
<br>
 +
 
 +
<h3>Anthocyanin composite part 2</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281102</a>
 +
<br>
 +
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 +
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
 
<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>
 
<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>
 
<br>
 
<br>
−
<b><i>3gt</i></b> Part BBa_waitingforsubmission
+
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
</p>
 
<br>
 
<br>
−
<b><i>yad</i>H</b> Part BBa_alsowaiting
+
<br>
 +
 
 +
<h3>Anthocyanin composite part 3>
 +
<p class="center">
 +
<a href="">BBa_K1281103</a>
 +
<br>
 +
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 +
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 +
<br>
 +
<b><i>3gt</i></b> Part <a href="">BBa_K1281003</a>
 +
<br>
 +
<b><i>yad</i>H</b> Part <a href="">BBa_K1281002</a>
 
<br>
 
<br>
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
These genes will all be used in the cell to produce Anthocyanin out of Eriodictyol. The <i>yad</i>H has been shown to increase yields even though it is not directly involved in the pathway. The <i>3gt</i> as well as <i>yad</i> H are two of our original basic part submissions. This Construct is our composite part submission BBa_anthocyanincompsitepart.
+
These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted
 
<br>
 
<br>
  
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<h2>&nbsp;Zeaxanthin Construct</h2>
 
<h2>&nbsp;Zeaxanthin Construct</h2>
−
<p class="center"> This construct is made up of the following genes:
+
<p class="center">
 +
<a href="">BBa_K1281301</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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<br>
 
<br>
  
−
<h2>&nbsp;Indigoidine Construct</h2>
+
<h2>&nbsp;Indigoidine Constructs</h2>
−
<p class="center">Our Indigoidine construct is made up of:
+
<h3>Indigoidine composite part 1</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281201</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 139: Line 172:
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>ind</i>B</b> Part <!--waiting for our part submission here-->BBa_waiting for actual part submission.
+
<b><i>ind</i>B</b> Part <a href="">BBa_K1281001</a>
 +
<br>
 +
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
</p>
 +
<br>
 +
<br>
 +
 
 +
<h3>Indigoidine composite part 2</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281202</a>
 +
<br>
 +
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 +
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
 
<b><i>ind</i>C</b> Part <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a>
 
<b><i>ind</i>C</b> Part <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a>
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<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
These two genes come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is one of our original basic part submissions. This construct is part BBa_idkyet
+
These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. This construct is part <a href="">BBa_K1281201</a>. We had to split our genes into tow separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.
 
<br>
 
<br>
 
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png"></center>
 
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png"></center>
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<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/f/f2/T--Lethbridge_HS--MelaninConstruct.png"></center>
 
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/f/f2/T--Lethbridge_HS--MelaninConstruct.png"></center>
 
</div>
 
</div>
−
 
 
</p>
 
</p>
 
<br>
 
<br>

Revision as of 23:03, 19 October 2017





 Anthocyanin Constructs



Anthocyanin composite part 1

BBa_K1281101
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
dfr Part BBa_K1497010
f3h Part BBa_K1497009
Terminator Part BBa_B0015.



Anthocyanin composite part 2

BBa_K1281102
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
ans Part BBa_K1497002
Terminator Part BBa_B0015.



Anthocyanin composite part 3>

BBa_K1281103
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
3gt Part BBa_K1281003
yadH Part BBa_K1281002
Terminator Part BBa_B0015.
These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted





 Zeaxanthin Construct

BBa_K1281301
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
crtY Part BBa_I742154.
crtZ Part BBa_I742157.
Terminator Part BBa_B0015.
These parts all come together in our composite part BBa_Zeaxanthinconstruct. This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.




 Indigoidine Constructs

Indigoidine composite part 1

BBa_K1281201
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indB Part BBa_K1281001
Terminator Part BBa_B0015.



Indigoidine composite part 2

BBa_K1281202
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indC Part BBa_K1152013
Terminator Part BBa_B0015.
These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The indB has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. This construct is part BBa_K1281201. We had to split our genes into tow separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.




 Melanin Construct

The parts used in our Melanin construct are as follows:
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
MelA Part BBA_K274001.
Terminator Part BBa_B0015.
This construct allows us to make Melanin out of L-Tyrosine. it is Part BBa_waitandsee