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Revision as of 09:52, 20 October 2017
Abstract
γ-Aminobutyric Acid (GABA) can be found in many plants and fermented foods, and plays the role of inhibitory neurotransmitter in human brain. Recently, GABA has been recognized as a natural relaxant as well as supplement to reduce anxiety levels and help combat stress. In order to reduce the cost of GABA production, our project aims to develop GABA-enriched yogurt through synthetic biology methods. Lactobacillus delbrueckii subsp. Bulgaricus is a common microorganism used in yogurt production and is able to produce GABA with low concentration. We propose to insert effective Lactobacillis promoters between gadR, gadA, and gadC (3 components of a high GABA producing species, Lactobacillus brevis NCL912). HPLC-UV measurement method will be used to evaluate the enhancement of GABA productivity in yogurt.
