Difference between revisions of "Team:WashU StLouis/Notebook"

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         <li> Prepared DH5α competent cells</li>
 
         <li> Prepared DH5α competent cells</li>
 
         <li> Tested MHD42 cells for competency</li>
 
         <li> Tested MHD42 cells for competency</li>
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         <li> Tested DH5α cells for competency</li>
 
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<script>
 
<script>

Revision as of 19:10, 6 June 2017

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Notebook

June 2017
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30

Micah, Maddie, Alex

  1. Prepared LB Media
  2. Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit
  3. Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells
  4. Prepared LB Agar
  5. Prepared overnight culture to make competent cells (MHD42)

Micah, Maddie

  1. Prepared MHD42 competent cells
  2. Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2
    • Transformation was a success with 100 colonies
    • Plate was stored in 4 deg. Celsius for future use
  3. Prepared TSS Buffer

No Work!

No Work!

Micah, Maddie, Alex, Mark, Collin

  1. Prepared Chloramphenicol (CM) plates
  2. Prepared Ampicillin solution to be used for plates in the future
  3. Made more LB Agar

Micah, Maddie, Alex, Mark, Collin

  1. Prepared DH5α competent cells
  2. Tested MHD42 cells for competency
  3. Tested DH5α cells for competency