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Revision as of 17:22, 21 October 2017




Characterization of a Thermal
Actuator and Calcium-Sensitive Reporter in
Saccharomyces cerevisiae



Project Description

Our current work was inspired by the ability of a thermosensitive ion channel, known as TRPV1, to import calcium into the cell in response to temperature changes. This process could one day be used to provide a method of In-Silico control of genetic circuits in synthetic biology. Our project explores the characterization of a calcium-sensitive reporter in Saccharomyces cerevisiae to monitor gene expression levels within a range of temperatures.

To learn more about our project, click here.





Project Aims

For our project this year we focused on characterizing the optimal extracellular calcium conditions to induce the expression of our calcium sensitive reporters. We are working with two reporter proteins that provide quick maturation rates and bright fluorescence within yeast so they will make for useful output indicators for In-Silico control in future work.

We also built on work from previous iGEM teams to further develop a deterministic model for the calcium pathway in yeast that will activate our reporter genes. Additionally, we developed a stochastic model to predict the nature of ion channel gating in response to temperature change. These models will help guide future work with TRPV1 as a thermal actuator in yeast.

To view our results, click here.


Human Practices

Description 2





Collaborations

Description 3





InterLab

Description 4





About Us

The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the iGEM Raiders team is composed of 11 undergraduate and graduate students.

To learn more about our team, click here.






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