Difference between revisions of "Team:TokyoTech/Experiment/TraI Assay"
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<h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px"><b>Overview</b></h1><!-- 小見出し --> | <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px"><b>Overview</b></h1><!-- 小見出し --> | ||
<hr style="width:50px;border:5px solid red" class="w3-round"> | <hr style="width:50px;border:5px solid red" class="w3-round"> | ||
| − | <p style="font-family: Poppins;font-size: 16px"> | + | <p style="font-family: Poppins;font-size: 16px">In this section we confirmed that E.coli containing Trail genes (we call it Seder E.coli) truly produce signal molecule, AHL. |
| + | At first to detect small amount of AHL we made reporter E.coli which respond to AHL and produce GFP. Then we studied AHL concentration dependence of reporter’s fluorescent intensity. | ||
| + | At last, we mix supernatant of Sender E.coli media with culture in which reporter grow and measure intensity of fluorescence and confirmed E.coli produce AHL (Acyl Homoserine Lactone). | ||
| + | |||
</p> | </p> | ||
</div> | </div> | ||
Revision as of 04:48, 22 October 2017
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TraI Assay
Overview
In this section we confirmed that E.coli containing Trail genes (we call it Seder E.coli) truly produce signal molecule, AHL. At first to detect small amount of AHL we made reporter E.coli which respond to AHL and produce GFP. Then we studied AHL concentration dependence of reporter’s fluorescent intensity. At last, we mix supernatant of Sender E.coli media with culture in which reporter grow and measure intensity of fluorescence and confirmed E.coli produce AHL (Acyl Homoserine Lactone).
Results
文章
文章
Discussion
考察
Reference
参考文献
Hajime Fujita: All Rights Reserved
