Line 91: | Line 91: | ||
<h1 class="brand-heading">Improve</h1> | <h1 class="brand-heading">Improve</h1> | ||
<p class="myintro-text"></p> | <p class="myintro-text"></p> | ||
− | <a href="# | + | <a href="#GFP |
" class="page-scroll"> | " class="page-scroll"> | ||
<div id="downarrow"> | <div id="downarrow"> |
Revision as of 14:17, 22 October 2017
GFP
The Golden Gate modular cloning system we worked with provides many advantages over other assembling standards. However, the wildly used GFP BBa_E0040 part is not compatible with this standard (RFC[1000]). Therefore, we decided to improve this part by silently mutating a BsaI restriction site to comply with RFC[1000]. Additionally we added the special RFC[1000] prefix and suffix with BsaI restriction sites. After digestion they create so called fusion sites, sticky ends which are specific for coding sequences: AATG at the 5’ end and GCTT at the 3’ end .
The new, RFC[1000] compatible part BBa_K2294444 was cloned into the pSB1C3 shipping vector.
FLPe + NLS
The new, RFC[10] compatible part BBa_K2294000 was cloned into the pSB1C3 shipping vector.