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− | < | + | <link href="https://2017.igem.org/Template:Vilnius-Lithuania/CSSvendor?action=raw&ctype=text/css" rel="stylesheet"/> |
− | < | + | <link href="https://2017.igem.org/Template:Vilnius-Lithuania/CSSprod?action=raw&ctype=text/css" rel="stylesheet"/> |
− | </ | + | </html> |
+ | {{Vilnius-Lithuania/navbar}} | ||
+ | <html> | ||
− | <div class=" | + | <div class="wrapper problem-page"> |
− | <h1> | + | <div class="container"> |
− | + | <h1>Present challenges of<br/> experimenting with plasmid systems</h1> | |
− | </ | + | <div class="presentation"> |
− | <p> | + | <div class="left"> |
− | < | + | <div class="left-steps"> |
− | + | <div class="left-step is-current" style="display: block"> | |
− | < | + | <div class="step">1. 1.</div> |
− | < | + | <div class="title">Pavadinimas</div> |
− | < | + | <p>It is often not possible to maintain multiple different plasmids which use the same |
− | </ | + | replication |
− | + | machinery.</p> | |
− | + | <h6>Problem #1</h6> | |
− | < | + | </div> |
− | + | <div class="left-step"> | |
− | < | + | <div class="step">1. 2.</div> |
− | + | <div class="title">Pavadinimas 2</div> | |
− | < | + | <p>Plasmids with growth advantages such as faster replication (due to their smaller size) have |
− | + | the potential to rapidly outgrow larger plasmids in the cell.</p> | |
− | + | <h6>Problem #1</h6> | |
− | <p>< | + | </div> |
− | + | <div class="left-step"> | |
− | + | <div class="step">1. 3.</div> | |
+ | <div class="title">Division</div> | ||
+ | <p> This is even more likely to occur when the plasmid copy number is low. When the cell | ||
+ | divides, lower copy plasmids tend to be lost in next generations. </p> | ||
+ | <h6>Problem #1</h6> | ||
+ | </div> | ||
+ | <div class="left-step"> | ||
+ | <div class="step">2. 1.</div> | ||
+ | <div class="title">Pavadinimas</div> | ||
+ | <p>One needs to use different types of Ori to avoid this trouble, however it often causes | ||
+ | dilemma due to limited selection options or the lack of good combination of Ori and | ||
+ | plasmid‘s copy number.</p> | ||
+ | <h6>Problem #2</h6> | ||
+ | </div> | ||
+ | <div class="left-step"> | ||
+ | <div class="step">2. 2.</div> | ||
+ | <div class="title">Pavadinimas</div> | ||
+ | <p>Imagine you require a system consisting of two - low and high copy plasmids. Try picking them | ||
+ | from the list above and see what happens.</p> | ||
+ | <h6>Problem #2</h6> | ||
+ | </div> | ||
+ | <div class="left-step"> | ||
+ | <div class="step">2. 3.</div> | ||
+ | <div class="title">Pavadinimas</div> | ||
+ | <p>Currently no handy method of changing the copy number of particular plasmid exists.</p> | ||
+ | <h6>Problem #2</h6> | ||
+ | </div> | ||
+ | <div class="left-step"> | ||
+ | <div class="step">2. 4.</div> | ||
+ | <div class="title">Pavadinimas</div> | ||
+ | <p>There is also no mechanism to adjust the copy number when so is required in any specific | ||
+ | situation: for example, incoming cellular signals or special patterns.</p> | ||
+ | <h6>Problem #2</h6> | ||
+ | </div> | ||
+ | <div class="left-step"></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="center"> | ||
+ | <div class="circle"> | ||
+ | <div class="slice is-current"> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot big-dot"></div> | ||
+ | <div class="content visible"> | ||
+ | <img id="p11" src="assets/images/T--Vilnius_Lithuania--step-1-1-center1.gif"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="slice"> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot big-dot"></div> | ||
+ | <div class="content"> | ||
+ | <img id="p12" src="assets/images/T--Vilnius_Lithuania--step-1-2-center1.gif"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="slice"> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot big-dot"></div> | ||
+ | <div class="content"> | ||
+ | <img id="p13" src="assets/images/T--Vilnius_Lithuania--step-1-3-center1.gif"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="slice"> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot big-dot"></div> | ||
+ | <div class="content"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-image1.png" width="381" height="185"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="slice"> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot big-dot"></div> | ||
+ | <div class="content"> | ||
+ | <div class="success-text" style="display: none">Success!</div> | ||
+ | <div class="error-text" style="display: none">Error!</div> | ||
+ | <div class="interactive-plasmid suc-err"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-dna.png" class="dna"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-int1.png" class="int1" width="42" | ||
+ | height="42"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-int2.png" class="int2" width="42" | ||
+ | height="42"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-int3.png" class="int3" width="42" | ||
+ | height="42"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-int4.png" class="int4" width="42" | ||
+ | height="42"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-int5.png" class="int5" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="success-text-long" style="display: none"> | ||
+ | As you can see from our example, even when one knows what copy number is needed for system, it is hard to find the right combination of compatible replicons and right copy number. | ||
+ | </div> | ||
+ | <div class="error-text-long" style="display: none"> | ||
+ | <div>Plasmids are incompatible, because they use the same replicons.</div> | ||
+ | <div class="button">Try again</div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="slice"> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot big-dot"></div> | ||
+ | <div class="content"> | ||
+ | <img class="i23center active" src="assets/images/T--Vilnius_Lithuania--step-2-3-centerhigh.png" width="381" height="185"> | ||
+ | <img class="i23center" src="assets/images/T--Vilnius_Lithuania--step-2-3-centermedium.png" width="381" height="185"> | ||
+ | <img class="i23center" src="assets/images/T--Vilnius_Lithuania--step-2-3-centerlow.png" width="381" height="185"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="slice"> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot big-dot"></div> | ||
+ | <div class="content"> | ||
+ | <div class="interactive-plasmid"> | ||
+ | <div class="wave"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-wave.png" width="94" height="63"> | ||
+ | </div> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-plasmid1.png" class="plasmid" width="48" height="48"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-plasmid2.png" class="plasmid" width="48" height="48"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-plasmid3.png" class="plasmid" width="50" height="48"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-plasmid1.png" class="plasmid" width="48" height="48"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-plasmid2.png" class="plasmid" width="48" height="48"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-plasmid3.png" class="plasmid" width="50" height="48"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-plasmid1.png" class="plasmid" width="48" height="48"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-plasmid2.png" class="plasmid" width="48" height="48"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-plasmid3.png" class="plasmid" width="50" height="48"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-4-plasmid1.png" class="plasmid" width="48" height="48"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-dna.png" class="dna"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="slice"> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot small-dot"></div> | ||
+ | <div class="dot big-dot"></div> | ||
+ | <div class="content"> | ||
+ | <h2>Did this look smart, easy or convenient?</h2> | ||
+ | <p>We think it is time for a change.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="mob-prev" style="background-image: url('https://static.igem.org/mediawiki/2017/a/a0/T--Vilnius-Lithuania--step-1-1-arrow.png')"></div> | ||
+ | <div class="mob-next" style="background-image: url('https://static.igem.org/mediawiki/2017/a/a0/T--Vilnius-Lithuania--step-1-1-arrow.png')"></div> | ||
+ | </div> | ||
+ | <div class="right"> | ||
+ | <div class="right-steps"> | ||
+ | <div class="right-step is-current" style="display: block"> | ||
+ | <div class="box"> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline1.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Plasmid A</div> | ||
+ | </div> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline2.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Plasmid B</div> | ||
+ | </div> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline3.png" width="12" | ||
+ | height="12"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Origin of replication</div> | ||
+ | </div> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline4.png" width="19" | ||
+ | height="19"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Replication machinery</div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="right-step"> | ||
+ | <div class="box"> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline1.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Plasmid A</div> | ||
+ | </div> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-2-boxline2.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Plasmid B</div> | ||
+ | </div> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline3.png" width="12" | ||
+ | height="12"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Origin of replication</div> | ||
+ | </div> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline4.png" width="19" | ||
+ | height="19"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Replication machinery</div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="right-step"> | ||
+ | <div class="box"> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline1.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Plasmid A</div> | ||
+ | </div> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-2-boxline2.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Plasmid B</div> | ||
+ | </div> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline3.png" width="12" | ||
+ | height="12"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Origin of replication</div> | ||
+ | </div> | ||
+ | <div class="box-line"> | ||
+ | <div class="imgcont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline4.png" width="19" | ||
+ | height="19"> | ||
+ | </div> | ||
+ | <div class="box-line-name">Replication machinery</div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="right-step"> | ||
+ | <div class="box"> | ||
+ | <div class="box-title">Different plasmids</div> | ||
+ | <div class="box-plasmids"> | ||
+ | <div class="box-plasmid"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-1-boxline1.png" width="42" | ||
+ | height="42"> | ||
+ | <p>A</p> | ||
+ | </div> | ||
+ | <div class="box-plasmid"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-plasmid1.png" width="42" | ||
+ | height="42"> | ||
+ | <p>B</p> | ||
+ | </div> | ||
+ | <div class="box-plasmid"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-plasmid2.png" width="42" | ||
+ | height="42"> | ||
+ | <p>C</p> | ||
+ | </div> | ||
+ | <div class="box-plasmid"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-1-2-boxline2.png" width="42" | ||
+ | height="42"> | ||
+ | <p>D</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="box"> | ||
+ | <div class="box-title">Different types of ORI</div> | ||
+ | <div class="box-sb"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-ori1.png" width="12" height="12"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-ori2.png" width="14" height="12"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-ori3.png" width="12" height="12"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-ori4.png" width="15" height="12"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="box"> | ||
+ | <div class="box-title">Different replication machinery molecules</div> | ||
+ | <div class="box-sb"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-mol1.png" width="18" height="18"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-mol2.png" width="21" height="18"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-mol3.png" width="18" height="18"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-1-mol4.png" width="21" height="18"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="right-step"> | ||
+ | <div class="interaction22"> | ||
+ | <div class="i22-heading"> | ||
+ | <p>Plasmid</p> | ||
+ | <p>Copy number</p> | ||
+ | </div> | ||
+ | <div class="i22-line" data-int="int1"> | ||
+ | <div class="img-cont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-int1.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="letter">A</div> | ||
+ | <div class="cn">High</div> | ||
+ | </div> | ||
+ | <div class="i22-line" data-int="int2"> | ||
+ | <div class="img-cont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-int2.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="letter">B</div> | ||
+ | <div class="cn">High</div> | ||
+ | </div> | ||
+ | <div class="i22-line" data-int="int3"> | ||
+ | <div class="img-cont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-int3.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="letter">C</div> | ||
+ | <div class="cn">Medium</div> | ||
+ | </div> | ||
+ | <div class="i22-line" data-int="int4"> | ||
+ | <div class="img-cont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-int4.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="letter">D</div> | ||
+ | <div class="cn">Low</div> | ||
+ | </div> | ||
+ | <div class="i22-line" data-int="int5"> | ||
+ | <div class="img-cont"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-2-int5.png" width="42" | ||
+ | height="42"> | ||
+ | </div> | ||
+ | <div class="letter">E</div> | ||
+ | <div class="cn">Low</div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="right-step"> | ||
+ | <div class="i23 active"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-3-righthigh.png" width="261" height="128"> | ||
+ | <span>High</span> | ||
+ | </div> | ||
+ | <div class="i23"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-3-rightmedium.png" width="261" height="128"> | ||
+ | <span>Medium</span> | ||
+ | </div> | ||
+ | <div class="i23"> | ||
+ | <img src="assets/images/T--Vilnius_Lithuania--step-2-3-rightlow.png" width="261" height="128"> | ||
+ | <span>Low</span> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="right-step"></div> | ||
+ | <div class="right-step"></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="solution-trigger"> | ||
+ | <div class="notice">Check our future solution! <img src="assets/images/T--Vilnius_Lithuania--step-2-5-line.png" width="77" height="52"></div> | ||
+ | <div class="the-trigger"></div> | ||
+ | <div class="text">SYNORI</div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </html> | ||
+ | <html> | ||
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Revision as of 10:40, 30 October 2017
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Present challenges of
experimenting with plasmid systems
It is often not possible to maintain multiple different plasmids which use the same replication machinery.
Problem #1
Plasmids with growth advantages such as faster replication (due to their smaller size) have the potential to rapidly outgrow larger plasmids in the cell.
Problem #1
This is even more likely to occur when the plasmid copy number is low. When the cell divides, lower copy plasmids tend to be lost in next generations.
Problem #1
One needs to use different types of Ori to avoid this trouble, however it often causes dilemma due to limited selection options or the lack of good combination of Ori and plasmid‘s copy number.
Problem #2
Imagine you require a system consisting of two - low and high copy plasmids. Try picking them from the list above and see what happens.
Problem #2
Currently no handy method of changing the copy number of particular plasmid exists.
Problem #2
There is also no mechanism to adjust the copy number when so is required in any specific situation: for example, incoming cellular signals or special patterns.
Problem #2
![](assets/images/T--Vilnius_Lithuania--step-1-1-center1.gif)
![](assets/images/T--Vilnius_Lithuania--step-1-2-center1.gif)
![](assets/images/T--Vilnius_Lithuania--step-1-3-center1.gif)
![](assets/images/T--Vilnius_Lithuania--step-2-1-image1.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-dna.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-int1.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-int2.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-int3.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-int4.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-int5.png)
![](assets/images/T--Vilnius_Lithuania--step-2-3-centerhigh.png)
![](assets/images/T--Vilnius_Lithuania--step-2-3-centermedium.png)
![](assets/images/T--Vilnius_Lithuania--step-2-3-centerlow.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-wave.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-plasmid1.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-plasmid2.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-plasmid3.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-plasmid1.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-plasmid2.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-plasmid3.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-plasmid1.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-plasmid2.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-plasmid3.png)
![](assets/images/T--Vilnius_Lithuania--step-2-4-plasmid1.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-dna.png)
Did this look smart, easy or convenient?
We think it is time for a change.
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline1.png)
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline2.png)
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline3.png)
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline4.png)
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline1.png)
![](assets/images/T--Vilnius_Lithuania--step-1-2-boxline2.png)
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline3.png)
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline4.png)
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline1.png)
![](assets/images/T--Vilnius_Lithuania--step-1-2-boxline2.png)
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline3.png)
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline4.png)
![](assets/images/T--Vilnius_Lithuania--step-1-1-boxline1.png)
A
![](assets/images/T--Vilnius_Lithuania--step-2-1-plasmid1.png)
B
![](assets/images/T--Vilnius_Lithuania--step-2-1-plasmid2.png)
C
![](assets/images/T--Vilnius_Lithuania--step-1-2-boxline2.png)
D
![](assets/images/T--Vilnius_Lithuania--step-2-1-ori1.png)
![](assets/images/T--Vilnius_Lithuania--step-2-1-ori2.png)
![](assets/images/T--Vilnius_Lithuania--step-2-1-ori3.png)
![](assets/images/T--Vilnius_Lithuania--step-2-1-ori4.png)
![](assets/images/T--Vilnius_Lithuania--step-2-1-mol1.png)
![](assets/images/T--Vilnius_Lithuania--step-2-1-mol2.png)
![](assets/images/T--Vilnius_Lithuania--step-2-1-mol3.png)
![](assets/images/T--Vilnius_Lithuania--step-2-1-mol4.png)
Plasmid
Copy number
![](assets/images/T--Vilnius_Lithuania--step-2-2-int1.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-int2.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-int3.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-int4.png)
![](assets/images/T--Vilnius_Lithuania--step-2-2-int5.png)
![](assets/images/T--Vilnius_Lithuania--step-2-3-righthigh.png)
![](assets/images/T--Vilnius_Lithuania--step-2-3-rightmedium.png)
![](assets/images/T--Vilnius_Lithuania--step-2-3-rightlow.png)
![](assets/images/T--Vilnius_Lithuania--step-2-5-line.png)