Skywalkerest (Talk | contribs) |
Skywalkerest (Talk | contribs) |
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− | < | + | <h2>1.Increase the production of B12 in B. megaterium</h2> |
1.1 Increase flux of glutamyl-tRNA to uroporphyrinogen Ⅲ by regulating feedback inhibition of glutamyl-tRNA reductase (HemA) | 1.1 Increase flux of glutamyl-tRNA to uroporphyrinogen Ⅲ by regulating feedback inhibition of glutamyl-tRNA reductase (HemA) | ||
1.2 Reduce flux of uroporphyrinogen Ⅲ to heme by an antisense RNA(as) strategy | 1.2 Reduce flux of uroporphyrinogen Ⅲ to heme by an antisense RNA(as) strategy | ||
1.3 Overexpress RdhANP to soak up excess B12 production | 1.3 Overexpress RdhANP to soak up excess B12 production | ||
− | < | + | <h2>2.Express and verify RdhANP</h2> |
2.1 Clone rdhANP to suitable vectors | 2.1 Clone rdhANP to suitable vectors | ||
2.2 Induce to express RdhANP in B. megaterium | 2.2 Induce to express RdhANP in B. megaterium | ||
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2.9 Test the dehalogenation of these mutants our constructed wastewater-imitating system | 2.9 Test the dehalogenation of these mutants our constructed wastewater-imitating system | ||
2.10 Coordinate B12 production and RdhANP expression | 2.10 Coordinate B12 production and RdhANP expression | ||
− | < | + | <h2>3.Bind B. megaterium to cellulose membrane</h2> |
3.1 Coexpress Cellulose binding domain(CBD) and Membrane anchoring domain(MAD) in B. megaterium | 3.1 Coexpress Cellulose binding domain(CBD) and Membrane anchoring domain(MAD) in B. megaterium | ||
3.2 Test the binding of B. megaterium to cellulose membrane | 3.2 Test the binding of B. megaterium to cellulose membrane | ||
− | < | + | <h2>4.Coexpress CBD, MAD and RdhANP in B. megaterium and test its binding to cellulose membrane and dehalogenation</h2> |
− | < | + | <h2>5.Construct biofilm containing RdhANP-expressing B. megaterium</h2> |
− | < | + | <h2>6.Introduce our engineered biofilm and cellulose membrane to HMBR</h2> |
<h2>7.Cooperate with JLU-iGEM to complete the halogenated phenol's degradation pathway and optimize organohalide-contained wastewater treatment</h2> | <h2>7.Cooperate with JLU-iGEM to complete the halogenated phenol's degradation pathway and optimize organohalide-contained wastewater treatment</h2> | ||
− | < | + | <h2>8.Introduce downstream devices to eliminate microorganisms leaked into the effluent</h2> |
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</body> | </body> | ||
</html> | </html> |
Revision as of 07:02, 23 October 2017