Skywalkerest (Talk | contribs) |
Skywalkerest (Talk | contribs) |
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<h2>1.Increase the production of B12 in B. megaterium</h2> | <h2>1.Increase the production of B12 in B. megaterium</h2> | ||
− | 1.1 Increase flux of glutamyl-tRNA to uroporphyrinogen Ⅲ by regulating feedback inhibition of glutamyl-tRNA reductase (HemA) | + | <h3>1.1 Increase flux of glutamyl-tRNA to uroporphyrinogen Ⅲ by regulating feedback inhibition of glutamyl-tRNA reductase (HemA) </h3> |
− | + | <h3>1.2 Reduce flux of uroporphyrinogen Ⅲ to heme by an antisense RNA(as) strategy </h3> | |
− | + | <h3>1.3 Overexpress RdhANP to soak up excess B12 production </h3> | |
<h2>2.Express and verify RdhANP</h2> | <h2>2.Express and verify RdhANP</h2> | ||
− | + | <h3>2.1 Clone rdhANP to suitable vectors </h3> | |
2.2 Induce to express RdhANP in B. megaterium | 2.2 Induce to express RdhANP in B. megaterium | ||
2.3 Purify and concentrate RdhANP | 2.3 Purify and concentrate RdhANP |
Revision as of 07:08, 23 October 2017
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