|
|
| Line 1: |
Line 1: |
| − | <html lang="en">
| |
| − |
| |
| − | <head>
| |
| − | <meta charset="UTF-8">
| |
| − | <meta http-equiv="X-UA-Compatible" content="IE=edge">
| |
| − | <meta content="width=device-width,initial-scale=1" name="viewport">
| |
| − | <meta content="description" name="description">
| |
| − | <meta name="google" content="notranslate" />
| |
| − |
| |
| − | <!-- Disable tap highlight on IE -->
| |
| − | <meta name="msapplication-tap-highlight" content="no">
| |
| − |
| |
| − | <style>
| |
| − |
| |
| − | /***************************************************** DEFAULT WIKI SETTINGS ****************************************************/
| |
| − | /* Clear the default wiki settings */
| |
| − | #home_logo, #sideMenu { display:none; } #sideMenu, #top_title, .patrollink {display:none;} #content { width:100%; padding:0px; margin-top:-7px; margin-left:0px;margin-right: 0;} body {background-color:white; } #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
| |
| − | .fa-icon1:hover{
| |
| − | color: black !important;
| |
| − | }
| |
| − | a:visited{
| |
| − | color:white;
| |
| − | }
| |
| − | .Menu{
| |
| − | background-color: #5f5f5f;
| |
| − | font-size: 3em;
| |
| − | text-align: center;
| |
| − | padding-top: 0.7em;
| |
| − | height: 2.2em;
| |
| − | text-decoration: none;
| |
| − | }
| |
| − | .Menu:hover{
| |
| − | background-color: #2b2b2b;
| |
| − | }
| |
| − |
| |
| − | .image-Menu{
| |
| − | width: 100%;
| |
| − | height: 100%;
| |
| − | }
| |
| − |
| |
| − | .h1, .h2, .h3, .h4, .h5, .h6, h1, h2, h3, h4, h5, h6 {
| |
| − | font-family: 'Dosis', sans-serif !important;
| |
| − | font-weight: 600 !important;
| |
| − | line-height: 1.1;
| |
| − | }
| |
| − | p {
| |
| − | font-family: 'Dosis', sans-serif !important;
| |
| − | font-weight: 400;
| |
| − | }
| |
| − | .p{
| |
| − | font-size: 0.87em !important;
| |
| − | }
| |
| − |
| |
| − | </style>
| |
| − |
| |
| − | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Amazonas_Brazil/CSS?action=raw&ctype=text/css">
| |
| − | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Amazonas_Brazil/CSS:icon?action=raw&ctype=text/css">
| |
| − |
| |
| − | <title>Wiki_iGEM_Amazonas</title>
| |
| − |
| |
| − | </head>
| |
| − |
| |
| − | <body style="width:100%; height:100%; padding: 0; margin: 0;">
| |
| − |
| |
| − | <!-- Add your content of header -->
| |
| − |
| |
| − | <header>
| |
| − | <nav class="navbar navbar-fixed-top navbar-default" style="background-color: rgba(255, 255, 255, 0);">
| |
| − | <ul style="opacity:0.95;list-style-type:none;margin:0;padding:0;background: linear-gradient(to left,#BD314C, #5F254A);overflow:hidden;position: fixed;top: 0;width: 100%;">
| |
| − | <li><a class="Link-pg" style="padding: 0 1em;" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png" width="104.04" height="90"style="margin-top: 0.3em;"></a></li>
| |
| − | </ul>
| |
| − |
| |
| − | <div class="container">
| |
| − | <button type="button" class="navbar-toggle" style="float: right;">
| |
| − | <span class="sr-only">Menu</span>
| |
| − | <span class="icon-bar" style="background-color: white"></span>
| |
| − | <span class="icon-bar" style="background-color: white"></span>
| |
| − | <span class="icon-bar" style="background-color: white"></span>
| |
| − | </button>
| |
| − |
| |
| − | <nav class="navbar-fullscreen" id="navbar-middle" style="opacity:0.9;">
| |
| − | <button type="button" class="close" aria-label="Close"><span aria-hidden="true" style="top: 0.3em; position: absolute;right: 0.05em;">×</span></button>
| |
| − |
| |
| − | <div class="main-container">
| |
| − | <div class="subcontainer" style="margin-top: 5em;">
| |
| − | <a class="Menu" style="margin-right: 0.5em; width: 50%; color: white; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/Project">PROJECT</a>
| |
| − | <a class="Menu" style="width: 50%; color: white; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/Biobricks">BIOBRICKS</a>
| |
| − | </div>
| |
| − | <div class="subcontainer">
| |
| − | <a class="Menu" style=" color: white;margin-right: 0.5em; width: 40%; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/Software">SOFTWARE</a>
| |
| − | <a class="Menu" style="width: 60%; color: white; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/Human_Practices">HUMAN PRACTICES</a>
| |
| − | </div>
| |
| − | <div class="subcontainer" style="margin-bottom: 1em;">
| |
| − | <a class="Menu" style="margin-right: 0.5em; width: 70%; color: white; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/Achievements">ACHIEVEMENTS</a>
| |
| − | <a class="Menu" style="width: 30%; color: white; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/People">PEOPLE</a>
| |
| − | </div>
| |
| − | </div>
| |
| − | </nav>
| |
| − | </div>
| |
| − | </nav>
| |
| − | </header>
| |
| − |
| |
| − |
| |
| − | <div class="hero-full-container background-image-container white-text-container" style="background: linear-gradient(to left,#BD314C, #5F254A); width:100%;">
| |
| − | <div class="container">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-12">
| |
| − | <div class="hero-full-wrapper">
| |
| − | <div class="text-content text-center" style="left: 0;margin-left: 0;margin-top: 23%;padding: 0">
| |
| − | <h2 align="middle" style="font-size: 3em; margin: -1em 0 0;padding: 0;">Software</h2>
| |
| − | <h3 align="middle" style="font-size: 1.5em; margin: 0;padding: 0;">A platform to expand the iGEM Experience</h2>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="section-container">
| |
| − | <div class="container">
| |
| − | <div class="col-xs-12">
| |
| − | <div class="row section-container-spacer">
| |
| − | <div class="col-xs-12">
| |
| − | <h2>CRISPeasy</h2>
| |
| − | <h3>Our perspective is to provide a bacterial genome editing vector based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR.
| |
| − | </h3>
| |
| − |
| |
| − | <h4 style="padding: 20px 0 0 0">What's the boost?</h4>
| |
| − | <div style="padding: 20px 0 0 0; text-align: justify;
| |
| − | text-justify: inter-word;">
| |
| − | <p class="p">Scientific community have been achieved great results using CRISPR-Cas9 mediated
| |
| − | genome editing techniques. While these methods are moving forward, some methodological
| |
| − | aspects related to this technique could be further improved to bring up this science
| |
| − | revolution even more.</p>
| |
| − | <p class="p">In prokaryotes, the genome editing based on CRISPR/Cas9 machinery require quite
| |
| − | laborious days of work in lab due to the multi-plasmid based edition. The system
| |
| − | não é padronizado e, em determinadas metodologias, demands more than one plasmid,
| |
| − | each one carrying a part of the CRISPR/Cas9 apparatus. Other critical problem consist
| |
| − | in the addition of a linear DNA carrying homologous arms to provide a donor
| |
| − | DNA to insert information into the genome during the HDR repair via. However,
| |
| − | the linear donor DNA system has low edition efficiency due the action of DNA restriction
| |
| − | systems present in wild type and E. coli strains, which can degrade the linear DNAs
| |
| − | before integration into genome. All this demands plenty of time and a lot of wet lab
| |
| − | hours of work. To verify this information, we got in touch with other iGEM's teams who
| |
| − | had work with CRISPR/Cas9 in the previous years, you can check this outreach part with
| |
| − | more details here.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <script>
| |
| − | document.addEventListener("DOMContentLoaded", function (event) {
| |
| − | navbarToggleSidebar();
| |
| − | closeMenuBeforeGoingToPage();
| |
| − | navActivePage();
| |
| − | });
| |
| − | </script>
| |
| − | <script type="text/javascript" src="https://2017.igem.org/Template:Amazonas_Brazil/JS?action=raw&ctype=text/javascript"></script>
| |
| − |
| |
| − | </body>
| |
| − |
| |
| − | <footer style="background: linear-gradient(to left,#BD314C, #5F254A);">
| |
| − | <div class="footer-container" >
| |
| − | <p class="footer-share-icons">
| |
| − | <a href="https://twitter.com/IGEM_Amazonas" class="fa-icon1" title="" style="padding-right: 0; background-color: transparent;">
| |
| − | <i class="fa fa-twitter" aria-hidden="true"></i>
| |
| − | </a>
| |
| − | <a href="https://www.instagram.com/igem_amazonas_brazil/" class="fa-icon1" title="" style="padding-right: 0; background-color: transparent;">
| |
| − | <i class="fa fa-instagram" aria-hidden="true"></i>
| |
| − | </a>
| |
| − | <a href="https://www.facebook.com/ManausnoiGEM/" class="fa-icon1" title="" style="padding-right: 0; background-color: transparent;">
| |
| − | <i class="fa fa-facebook" aria-hidden="true"></i>
| |
| − | </a>
| |
| − | </p>
| |
| − | </div>
| |
| − | </footer>
| |
| − |
| |
| − | </html>
| |
| | <html lang="en"> | | <html lang="en"> |
| | | | |
