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| | + | <head><meta name="viewport" content="width=device-width, initial-scale=1"><meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><script>function disputeIframe(){setTimeout(function (){self.document.open();self.document.write("<h1><a style='font-weight: normal; color: #000; font-family: arial;' href='" + self.location.href + "' target='_top'>Iframes are not supported on a free plan.</a></h1>"); self.document.close();},200);} try {if (self!=top){disputeIframe();}}catch(e){disputeIframe();}</script><link href="//d33rxv6e3thba6.cloudfront.net/asset/sites/css/bootstrap3.min.css" rel="stylesheet"><link rel="stylesheet" href="//d33rxv6e3thba6.cloudfront.net/asset/sites/css/font-awesome.min4.css"><link href="//d33rxv6e3thba6.cloudfront.net/asset/sites/css/hover-min.css" rel="stylesheet"><link href="/static/css/style.css?v=2.0.4" rel="stylesheet"><link href="/static/css/blocks.css?v=2.0.4" rel="stylesheet"><link href="/static/css/theme-css/ez.css?v=2.0.4" rel="stylesheet"><style type='text/css' data='Page Custom CSS'></style><script src="//code.jquery.com/jquery-1.12.4.min.js" integrity="sha256-ZosEbRLbNQzLpnKIkEdrPv7lOy9C27hHQ+Xp8a4MxAQ=" crossorigin="anonymous"></script><script> if (!window.jQuery) { document.write('<script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/jquery.js"></scr' + 'ipt>'); }</script><meta name="page-source" site="//sites.simbla.com/52b5a023-1bcf-d512-a412-3351ef830f04" page="design" site-id="593aca5737cad4ed7fa1fc16" page-id="59416e8195f86d9166bcfe25" master-page-id="" /><link rel="canonical" href="//sites.simbla.com/52b5a023-1bcf-d512-a412-3351ef830f04/design" /></head><body data-spy="scroll" data-target=".navbar-collapse" class="static-page"><div id="windowDiv"><div id="MarginsTop" style="height: 0px;" class="row"></div><div id="bodyContainer" style="width:100%"><div class="simblaEL simblaBlock headerBlock containerHolder" data-drag="P0" id="P0" style="position: relative;"><a class="simblaEL anchorElm" data-drag="divCounter" name="Intro0" id="Intro0"></a><div class="container" style="width: 100%; padding: 0px;"><header class="simblaEL container-fluid" data-drag="P2" id="P2"><div class="row simblaEL rDivider" data-drag="P4" id="P4" style="position: relative;"><div class="sDivider col-md-2 index0" data-colsize="2"></div><div class="sDivider col-md-10 index1" data-colsize="10"></div></div></header></div></div><div class="simblaEL containerHolder" data-drag="P0" id="P0" style=""><a class="simblaEL anchorElm" data-drag="divCounter" name="Intro0" id="Intro0"></a><div class="container-fluid minHeight60 active" data-border-type="All" style="overflow: hidden; min-height: 416.4px; border-radius: 0px; background-image: url("//d33rxv6e3thba6.cloudfront.net/2015/10/5648949c3a571f390c74a1cb/3153-1yh8305.png"); background-attachment: initial; background-color: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: repeat;"><div class="simblaEL containerHolder" data-drag="P10" id="P10" style="position: relative;"><div class="container" style=""><div class="row simblaEL rDivider" data-drag="P12" id="P12" style="position: relative; padding-top: 30px;"><div class="sDivider col-md-6 index0" data-colsize="6"><div class="simblaEL tcH" data-drag="P14" id="P14" style="position: relative;"><div class="textContainer H1" style="cursor: text; padding-top: 80px;"><h1>Design</h1></div></div><div class="simblaEL tcH" data-drag="P16" id="P16"><div class="textContainer H2" style="cursor: text;"><h2><div style="text-align: left;">The foundation of our circuit</div></h2></div></div><div class="simblaEL tcH" data-drag="P18" id="P18" style="position: relative;"><div class="textContainer H3" style="cursor: text; padding-bottom: 30px; padding-right: 20px;"><h3><font style="font-size: 24px;" color="#444444"> |
| | + | Our circuit was designed to utilize specific parts so that when the E-coli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light. </font><div><br></div></h3></div></div></div><div class="sDivider col-md-6 index1" data-colsize="6"><div class="simblaEL simblaImg" data-drag="P20" id="P20"><img draggable="false" class="" alt="" data-id="" title="" src="https://s3.amazonaws.com/simbla-static/2017/5/593aca5737cad4ed7fa1fc16/12707-ymd99p.jpg"></div></div></div></div></div></div></div><div style="position: relative;" id="P21" class="simblaEL simblaBlock contentBlock content11 containerHolder" data-drag="P21"><a class="simblaEL anchorElm" data-drag="divCounter" name="Content0" id="Content0" data-id="594172e45e8cf097669ba07b"></a><div style="border-radius: 0px; width: 100%; margin-top: 35px;" data-border-type="All" class="container"><div style="position: relative;" class="simblaEL containerHolder" data-drag="P23" id="P23"><div class="container" style="padding: 0px; margin: 0px auto;"><div class="row simblaEL rDivider" data-drag="P25" id="P25" style=""><div class="sDivider col-md-6 index0" data-colsize="6" style=""><div class="row main simblaEL rDivider" data-drag="P27" id="P27" style="padding: 20px 25px 25px; margin-bottom: 25px; border-radius: 4px;" data-border-type="All"><div class="sDivider col-md-2 index0" data-colsize="2" style=""><div class="simblaEL iconElm" data-drag="P29" id="P29" style="padding: 0px; margin-top: 20px; margin-right: 0px; margin-bottom: 0px;"><i class="fa fa-anchor bicon" style="" data-color="#ffffff" data-bgcolor="#2e3144" data-shadow="s5" data-border="circle borderI"></i></div></div><div class="sDivider col-md-10 index1" data-colsize="10"><div class="simblaEL tcH" data-drag="P31" id="P31"><div class="textContainer H2" style="cursor: text;"><h2>PHASE 1</h2></div></div><div class="simblaEL tcH" data-drag="P33" id="P33"></div><div class="simblaEL tcH" data-drag="P35" id="P35"></div><div class="simblaEL tcH" data-drag="P37" id="P37"></div><div class="simblaEL tc" data-drag="P39" id="P39"><div class="textContainer" style="cursor: text; line-height: 24px;"><p style="line-height: 24px;"></p><span style="color: rgb(255, 255, 255); line-height: 24px;"><ul><li><font style="font-size: 24px;">Test efficacy of the circuit in vivo <br></font></li><li><font style="font-size: 24px;">Grow transformed e.coli with plasmid circuit in Arsenic laced media <br></font></li><li><font style="font-size: 24px;">Serial dilution of arsenic and arsenite to find the lowest activation amount of the circuit in vivo</font><br></li></ul></span><p style="line-height: 24px;"></p></div></div><div class="simblaEL tc" data-drag="P41" id="P41" style="position: relative;"></div></div></div></div><div class="sDivider col-md-6 index1" data-colsize="6" style=""><div class="row simblaEL main rDivider" data-drag="P43" id="P43" style="padding: 20px 25px 25px; margin-bottom: 25px; position: relative; border-radius: 4px;" data-border-type="All"><div class="sDivider col-md-2 index0" data-colsize="2" style=""><div class="simblaEL iconElm" data-drag="P45" id="P45" style="padding: 0px; margin-top: 20px; margin-right: 0px; margin-bottom: 0px;"><i class="fa fa-cogs bicon" style="" data-color="#ffffff" data-bgcolor="#2e3144" data-shadow="s5" data-border="circle borderI"></i></div></div><div class="sDivider col-md-10 index1" data-colsize="10"><div class="simblaEL tcH" data-drag="P47" id="P47"></div><div class="simblaEL tcH" data-drag="P49" id="P49"></div><div class="simblaEL tcH" data-drag="P51" id="P51"><div class="textContainer H2" style="cursor: text;"><h2>PHASE 2</h2></div></div><div class="simblaEL tc" data-drag="P53" id="P53"><div class="textContainer" style="cursor: text; line-height: 24px;"><p style="line-height: 24px;"></p><ul><li style="color: rgb(255, 255, 255);"><font style="font-size: 24px;" color="#ffffff">Test the efficacy of the plasmid circuit in situ <br></font></li><li style="color: rgb(255, 255, 255);"><font style="font-size: 24px;" color="#ffffff">Perform above experiments with minipreped plasmid circuit <br></font></li><li style="color: rgb(255, 255, 255);"><font style="font-size: 24px;" color="#ffffff">Titrate both circuit and trigger to find optimal mixture <br></font></li><li style="color: rgb(255, 255, 255);"><font style="font-size: 24px;" color="#ffffff">Standardize reactions <br></font></li><li style="color: rgb(255, 255, 255);"><font style="font-size: 24px;" color="#ffffff">Test on paper matrix</font><br></li></ul><p></p></div></div><div class="simblaEL tc" data-drag="P55" id="P55"></div></div></div></div></div><div class="row simblaEL rDivider" data-drag="P57" id="P57" style="padding: 0px; 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line-height: 24px;"><p style="line-height: 24px;"></p><ul><li style="color: rgb(255, 255, 255);"><font style="font-size: 24px;" color="#ffffff">Test efficacy of freeze drying circuit on paper for distribution and storage purposes. </font></li></ul><p></p><p style="line-height: 24px;"><br></p></div></div><div class="simblaEL tc" data-drag="P71" id="P71"></div></div></div></div><div class="sDivider col-md-6 index1" data-colsize="6" data-title="Column"></div></div></div></div></div></div></div></div><a href="https://www.simbla.com" target="_blank" id="linkToSimbla" class="en"><span class="main">SIMBLA</span><span class="s">Website Builder</span></a><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/jquery.bootpag.min.js"></script><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/bootstrap3.min.js"></script><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/Chart.min.js"></script><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/numeral.min.js"></script><script src="/static/js/simbla.js?v=2.0.4"></script><script src="https://www.google.com/recaptcha/api.js?onload=onloadRecaptcha&render=explicit" async defer></script></body> |
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| − | class="wsite-menu-item"
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| − | <div class="paragraph" style="text-align:center;"><font size="5">Our circuit was designed to utilize specific parts so that when the E-coli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.</font><br /></div>
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| − | <img src="/uploads/1/0/7/7/107726947/arsnic-redesing-1-1_2_orig.png" alt="Picture" style="width:auto;max-width:100%" />
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| − | <div class="paragraph"><strong><u>Phase one</u></strong><ul><li>Test efficacy of the circuit in vivo<ul><li>grow transformed e.coli with plasmid circuit in Arsenic laced media</li><li>Serial dilution of arsenic and arsenite to find the lowest activation amount of the circuit in vivo</li></ul></li></ul> <strong><u>Phase two</u></strong><ul><li>Test the efficacy of the plasmid circuit in situ<ul><li>perform above experiments with minipreped plasmid circuit<br /></li><li>Titrate both circuit and trigger to find optimal mixture</li><li>standardize reactions </li><li>Test on paper matrix<br /></li></ul></li></ul> <strong><u>Phase three</u></strong><br />Test efficacy of freeze drying circuit on paper for distribution and storage purposes. </div>
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