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− | <a href="#!" onclick="toggleHeight6(this, | + | <a href="#!" onclick="toggleHeight6(this, 600); return false" |
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Suicide Mechanism Functional Test | Suicide Mechanism Functional Test | ||
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+ | <p> Before we construct our suicide mechanism with NrtA, we want to make sure that our suicide mechanism, endolysin and holin, can be induced by lactose efficiently. Therefore, we test our suicide mechanism by adding different concentration of lactose in order to find out the minimum effective concentration. Furthermore, to induce <i>E.coli</i> suicide at the time we want, we also want to know the changing degree of the OD value of bacterium at different time after the lactose is added.</p> | ||
+ | <p> We measure the OD<sub>600</sub> value of the sample, and then aliquot liquid culture into several cuvettes. Next, we add different amount of 0.5mM lactose into cuvettes to form different concentration of lactose, and measure the OD<sub>600</sub> value per hour.</p> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/4/4d/T--NYMU-Taipei--NS_suicide_func.png" style="width:95%"> | ||
+ | <p> As the figure shows, the suicide mechanism is induced immediately when lactose is added into the samples. Besides, we can see that the lactose concentration and the degree of decline of OD value are positively correlated.</p> | ||
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Revision as of 15:04, 25 October 2017