Rstclair2012 (Talk | contribs) |
Rstclair2012 (Talk | contribs) |
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<center><h4>Member Participations</h4></center> | <center><h4>Member Participations</h4></center> | ||
<h5>Calibrations:</h5> | <h5>Calibrations:</h5> | ||
− | - Completed by Douglas + Valentina on September 21st | + | <p>- Completed by Douglas + Valentina on September 21st</p> |
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<h5>Transformations:</h5> | <h5>Transformations:</h5> | ||
− | - Completed by Douglas + Ariania and supervised by Dr. Pavlovic on September 26th | + | <p>- Completed by Douglas + Ariania and supervised by Dr. Pavlovic on September 26th</p> |
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<h5>Cell Measurement:</h5> | <h5>Cell Measurement:</h5> | ||
− | - Completed by Douglas + Rachel S. and assisted by Eric and Daniela (Esiobu lab members) on September 27th, 28th, 29th | + | <p>- Completed by Douglas + Rachel S. and assisted by Eric and Daniela (Esiobu lab members) on September 27th, 28th, 29th</p> |
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<h6>Devices (from InterLab Measurement Kit): </h6> | <h6>Devices (from InterLab Measurement Kit): </h6> | ||
− | ● Positive control | + | <p>● Positive control |
<br/> | <br/> | ||
● Negative control | ● Negative control | ||
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● Test Device 5: J23106.BCD2.E0040.B0015 | ● Test Device 5: J23106.BCD2.E0040.B0015 | ||
<br/> | <br/> | ||
− | ● Test Device 6: J23117.BCD2.E0040.B0015 | + | ● Test Device 6: J23117.BCD2.E0040.B0015</p> |
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<h5>Calibration Procedure</h5> | <h5>Calibration Procedure</h5> | ||
− | ◻ Added 100 µl LUDOX into wells A1, B1, C1, D1 (or 1 mL LUDOX into cuvette) | + | <p>◻ Added 100 µl LUDOX into wells A1, B1, C1, D1 (or 1 mL LUDOX into cuvette) |
<br/> | <br/> | ||
◻ Added 100 µl of H2O into wells A2, B2, C2, D2 (or 1 mL H2O into cuvette) | ◻ Added 100 µl of H2O into wells A2, B2, C2, D2 (or 1 mL H2O into cuvette) | ||
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◻ grew overnight at 37C | ◻ grew overnight at 37C | ||
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− | ◻ this was done for all 8 machines | + | ◻ this was done for all 8 machines</p> |
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transform Escherichia coli DH5α with these following plasmids: | transform Escherichia coli DH5α with these following plasmids: | ||
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− | ● Positive control | + | <p>● Positive control |
<br/> | <br/> | ||
● Negative control | ● Negative control | ||
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● Test Device 5: J23106.BCD2.E0040.B0015 | ● Test Device 5: J23106.BCD2.E0040.B0015 | ||
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− | ● Test Device 6: J23117.BCD2.E0040.B0015 | + | ● Test Device 6: J23117.BCD2.E0040.B0015</p> |
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<h6>Day 2:</h6> | <h6>Day 2:</h6> | ||
− | + | <p> Picked 2 colonies from each of plate and inoculate it on 5-10 mL LB medium + Chloramphenicol. | |
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Grew the cells overnight (16-18 hours) at 37°C and 220 rpm. | Grew the cells overnight (16-18 hours) at 37°C and 220 rpm. | ||
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measurement) | measurement) | ||
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− | ◻ Recorded data in notebook | + | ◻ Recorded data in notebook</p> |
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<center><h4>Data</h4></center> | <center><h4>Data</h4></center> |
Revision as of 19:55, 26 October 2017
Florida_Atlantic