Difference between revisions of "Team:Lethbridge HS/Part Collection"

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<div class="container-fluid" style="margin-left:10%; margin-right:10%;">
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<div class="container-fluid" style="margin-left:10%; margin-right:10%;">  
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<h1><b>Basic Parts:</b></h1>
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<h2><b>&nbsp;Anthocyanin</b></h2>
 
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<h3><i>3gt</i></h3>
 
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<p class="center"> The gene <i>3gt</i> is from the anthocyanin synthesis pathway and converts the initial molecule Pelargonidin into Anthocyanin. This gene is from the organism <i>Petunia hybrid</i>. We have added this parts sequence to the registry for use in our composite part <a href="">BBa_K1281103</a>. The basic part is <a href="">BBa_K1281003</a>
 
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</p>
 
  
  
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<h3><i>yad</i>H</h3>
 
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<p class="center">This Gene is an <i>Escherichia coli</i> gene that has been shown to increase the yields of anthocyanin when paired with the genes in our anthocyanin construct. We have added the sequence to the registry for use in our composite part <a href="">BBa_K1281103</a>. The basic part is <a href="">BBa_K1281002</a>
 
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</p>
 
  
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<h3><i>f3h</i></h3>
 
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<p class="center">We will be useing the gene <i>f3h</i> as the first gene in our anthocyanin synthesis pathway, it comes from the organism <i>Petroselinum crispum</i>. It was added to the registry by the 2014 Darmstadt iGEM team, part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>. This gene will code for a protein that converts the initial molecule flavanone into dihydroflavonol.
 
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</p>
 
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<h3><i>dfr</i></h3>
 
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<p class="center">The gene <i>dfr</i> is the second one in our anthocyanin synthesis pathway. We are using the biobrick part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>. It was added to the registry by the 2014 Darmstadt igem team. </p>
 
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<h3><i>ans</i></h3>
 
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<p class="center">This gene is the third gene in our pathway, it converts the molecule created by dfr into pelargonidin. It is from the organism <i>Fragaria x ananassa</i> and was added to the registry by the 2014 Darmstadt team. It is an engineered anthocyanidin synthase. Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>.
 
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</p><br>
 
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<h2><b>&nbsp;Zeaxanthin</b></h2>
 
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<h3><i>crt</i>Y</h3>
 
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<p class="center">This gene is from the organism <i>Pantoea ananatis</i> and is part or the carotenoid synthesis pathway. It is a Lycopene cyclase and converts the initial molecule Lycopene into the final molecule Beta-Carotene. This gene was added to the registry by Edinburgh 2007, part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I742154">BBa_I742154</a>.
 
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</p>
 
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<h3><i>crtZ</i></h3>
 
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<p class="center">This gene is from the organism <i>Pantoea ananatis</i> and is our final gene in the carotenoid synthesis pathway. It is the beta-carotene hyroxylase, and converts Beta-carotene into Zeaxanthin. It was added to the registry by Edinburgh 2007 and is the part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I742157">BBa_I742157</a>.This part converts the Beat-Carotene into our final product, the pigment Zeaxanthin.
 
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</p><br>
 
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<h2><b>&nbsp;Melanin</b></h2>
 
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<h3><i>mel</i>A</h3>
 
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<p class="center">The gene melA is from the organism <i>Rhizobium etli</i> and was added to the registry by the Cambridge 2009 iGEM team. Part <a href="http://parts.igem.org/Part:BBa_K274001">BBa_K274001</a>. This protein is a tyrosinase and converts the molecule L-tyrosine into dopaquinone, which will then polymerize into Melanin.
 
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</p><br>
 
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<h2><b>&nbsp;Indigoidine</b></h2>
 
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<h3><i>ind</i>B</h3>
 
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<p class="center">This gene is from the organism <i>Streptomyces chrmofuscus</i>, and it is our original basic part submission to the registry. Its is part <a href="">BBa_K1281001</a>. It has been shown to increase the yeilds of Indigoidine when used with <i>indC</i>. It is a putative phosphatase.
 
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</p>
 
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<h3><i>ind</i>C</h3>
 
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<p class="center">This gene is the gene that converts Glutamine into Indigoidine. It is from the organism <i>Photohabdus luminescens</i> and was added to the registry by the 2013 Heidelburg iGEM team. It is the indigoidine synthase and converts Glutamine thioester into the molecule %-amino-3H-pyridine-2,6-dione, two of those molecules then condense into Indigoidine. Part <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a>.
 
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</p><br>
 
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<h2><b>&nbsp;Additional parts used</b></h2>
 
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<h3>T7 Promoter</h3>
 
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<p class="center">The Promoter we used for our Melanin Construct is  T7 promoter, This allows us to control the production of Melanin. This promoter is from the T7 bacteriophage, it is a Virus that inserts its DNA into Bacteria in order to reproduce and stay alive. This promoter works with the T7 system inside the <i>Escherichia coli</i> strain BL21(DE3), (Fig 1.)
 
 
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<img src="https://static.igem.org/mediawiki/2017/d/dc/T--Lethbridge_HS--T7System.png" class="img-responsive" >
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<h1><b>Composite parts used</b></h1>
 
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(Fig 1.) This image shows the T7 Promoter system, 1.Lactose inducible promoter 2.<i>E. coli</i> Ribosomal Binding site. 3.The Gene for T7 RNA polymerase. 4.Terminator 5.T7 RNA polymerase. 6.T7 promoter. 7.<i>E. coli</i> Ribosomal Binding site. 8.The gene/genes in our construct. 9.Terminator. 10.mRNA produced by the transcription of the construct.
 
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This shows the process that occurs with the T7 System. We induce the Lactose inducible promoter(1) with IPTG as it mimics the lactose and cannot be broken down by the cell. This allows it to continually perform transcription on the construct(1-4) embedded in the Genome of the BL21(DE3) and thusly constantly produce the T7 RNA polymerase(5) encoded by the gene(3).This T7 RNA Polymerase(5) attaches to the T7 Promoter(6) and starts transcription on the construct(6-9) within our plasmid psB1C3. This then creates the mRNA(10) which will undergo translation and produce the protein encoded by the gene(8) due to the <i>E. coli</i> RBS on the construct. Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
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</p><br>
 
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<h3>&nbsp;Ribosomal Binding Site</h3>
 
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<p class="center">The RBS we have chosen for our constructs is an <i>E. coli</i> Ribosomal Binding Site, Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>
 
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</p><br>
 
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<h3>&nbsp;Terminator</h3>
 
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<p class="center">The terminator we have chosen for our constructs is an <i>E. coli</i> terminator. Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>, it is a double terminator made up of one <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0012">BBa_B0012</a> terminator and a <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0010">BBa_B0010</a> terminator.
 
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</p>
 
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<h1><b>Composite Parts:</b></h1>
 
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<br>
 
 
<h2><b>&nbsp;Anthocyanin Constructs</b></h2>
 
<h2><b>&nbsp;Anthocyanin Constructs</b></h2>
 
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<h3>Anthocyanin composite part 1</h3>
 
<h3>Anthocyanin composite part 1</h3>
 
<p class="center">
 
<p class="center">
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<a href="">BBa_K1281101</a>
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<a href="">BBa_K2481113</a>
 
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<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
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<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>
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<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481110">BBa_K2481110</a>
 
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<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>
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<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481111">BBa_K2481111</a>
 
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<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
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<h3>Anthocyanin composite part 2</h3>
 
<h3>Anthocyanin composite part 2</h3>
 
<p class="center">
 
<p class="center">
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<a href="">BBa_K1281102</a>
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<a href="">BBa_K2481114</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
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<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>
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<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481112">BBa_K2481112</a>
 
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<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
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<h3>Anthocyanin composite part 3</h3>
 
<h3>Anthocyanin composite part 3</h3>
 
<p class="center">
 
<p class="center">
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<a href="">BBa_K1281103</a>
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<a href="http://parts.igem.org/Part:BBa_K2481105">BBa_K2481105</a>
 
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<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
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<b><i>3gt</i></b> Part <a href="">BBa_K1281003</a>
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<b><i>3gt</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481002">BBa_K2481002</a>
 
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<b><i>yad</i>H</b> Part <a href="">BBa_K1281002</a>
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<b><i>yad</i>H</b> Part <a href="http://parts.igem.org/Part:BBa_K2481004">BBa_K2481004</a>
 
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<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
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<h3>Zeaxanthin composite part</h3>
 
<h3>Zeaxanthin composite part</h3>
 
<p class="center">
 
<p class="center">
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<a href="">BBa_K1281301</a>
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<a href="http://parts.igem.org/Part:BBa_K2481107">BBa_K2481107</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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<h3>Indigoidine composite part 1</h3>
 
<h3>Indigoidine composite part 1</h3>
 
<p class="center">
 
<p class="center">
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<a href="">BBa_K1281201</a>
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<a href="http://parts.igem.org/Part:BBa_K2481106">BBa_K2481106</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
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<b><i>ind</i>B</b> Part <a href="">BBa_K1281001</a>
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<b><i>ind</i>B</b> Part <a href="http://parts.igem.org/Part:BBa_K2481001">BBa_K2481001</a>
 
<br>
 
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<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
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<h3>Indigoidine composite part 2</h3>
 
<h3>Indigoidine composite part 2</h3>
 
<p class="center">
 
<p class="center">
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<a href="">BBa_K1281202</a>
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<a href="http://parts.igem.org/Part:BBa_K2481109">BBa_K2481109</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. This construct is part <a href="">BBa_K1281201</a> and part <a href="">BBa_K1281202</a>. We had to split our genes into tow separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.
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These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. We had to split our genes into two separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.
 
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<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png"></center>
 
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png"></center>
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<h2><b>&nbsp;Melanin Construct</b></h2>
 
<h2><b>&nbsp;Melanin Construct</b></h2>
 
<h3>Melanin composite part</h3>
 
<h3>Melanin composite part</h3>
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<p class="center">The parts used in our Melanin construct are as follows:
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<p class="center">
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<a href="http://parts.igem.org/Part:BBa_K2481108">BBa_K2481108</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.

Revision as of 18:00, 28 October 2017







Composite parts used


 Anthocyanin Constructs


Anthocyanin composite part 1

BBa_K2481113
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
dfr Part BBa_K2481110
f3h Part BBa_K2481111
Terminator Part BBa_B0015.


Anthocyanin composite part 2

BBa_K2481114
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
ans Part BBa_K2481112
Terminator Part BBa_B0015.


Anthocyanin composite part 3

BBa_K2481105
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
3gt Part BBa_K2481002
yadH Part BBa_K2481004
Terminator Part BBa_B0015.

These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted





 Zeaxanthin Construct

Zeaxanthin composite part

BBa_K2481107
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
crtY Part BBa_I742154.
crtZ Part BBa_I742157.
Terminator Part BBa_B0015.

This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.



 Indigoidine Constructs

Indigoidine composite part 1

BBa_K2481106
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indB Part BBa_K2481001
Terminator Part BBa_B0015.


Indigoidine composite part 2

BBa_K2481109
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indC Part BBa_K1152013
Terminator Part BBa_B0015.

These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The indB has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. We had to split our genes into two separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.




 Melanin Construct

Melanin composite part

BBa_K2481108
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
MelA Part BBA_K274001.
Terminator Part BBa_B0015.

This construct allows us to make Melanin out of L-Tyrosine.