Difference between revisions of "Team:ETH Zurich/Parts"

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<summary>BBa_K2500008: Pconst_RBSeng_TlpA</summary>
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<p>Description.</p>
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<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500008">BBa_K2500008</a></p>
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Revision as of 07:42, 27 October 2017

Parts

Overview

We submitted in total 14 new BioBricks to the iGEM registry! You can find all parts and their design below.

Basic Parts

Part Description BioBrick
Bacterioferritin Heme-deletion mutant of bacterial iron storage protein functioning as MRI contrast agent BBa_K2500000
Azurin Mature cytotoxic copper protein BBa_K2500001
p28 Effector domain of Azurin BBa_K2500002
PtlpA Best Basic Part: Temperature-responsive promoter TlpA optimized for slight activation above 37°C and full activation at 45 °C BBa_K2500003
TlpA Protein reversibly inhibiting the pTlpA promoter at temperatures below 37 °C BBa_K2500004
AND gate a Synthetic promoter responsive to LldR and luxR BBa_K2500010
AND gate b Synthetic promoter responsive to LldR and luxR BBa_K2500011
AND gate c Synthetic promoter responsive to LldR and luxR BBa_K2500012

Composite Parts

Part Description BioBrick
Pconst_RBSeng_TlpA Best Composite Part:Temperature-dependent transcriptional repressor TlpA regulated by constant promoter and engineered RBS BBa_K2500008
pTlpA_Protein E Bacterial cell lysing protein E regulated by heat-inducible promoter pTlpA and synthetic RBS BBa_K2500009
Pconst_RBS_LldP/
LldR_Pconst_RBS_LuxR		 Expression cassette consisting of LuxR and LldP/LldR BBa_K2500013

Design

BBa_K2500008: Pconst_RBSeng_TlpA

Description.

Registry: BBa_K2500008

BBa_K2500010: AND gate a

In absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the binding sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcription. In design a, the distances between the intercalated promoter and the binding sites were adapted from BBa_K1847007.

Registry: BBa_K2500010

BBa_K2500011: AND gate b

In design b, each binding site was duplicated in order to achieve a potential zipper mechanism and stronger inhibition due to binding more LldR inhibitor proteins.

Registry: BBa_K2500011

BBa_K2500012: AND gate c

In design c, an artificial spacer was embedded between the pLux promoter and the O2 binding site in order to influence the looping dynamics.

Registry: BBa_K2500012