Difference between revisions of "Team:Cadets2Vets/Demonstrate"

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Demonstrate - CADETS2VETS</title>
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<div class='ParagraphBox ' style='position:absolute;left:583px;top:11px;z-index:10008;padding-left:15px;padding-right:28px;padding-top:12px;padding-bottom:13px;width:429px;height:572px;overflow:hidden;background-color:rgba(250, 8, 40 , 0.85);background-position-x:undefined;background-position-y:undefined;background-repeat:undefined;background-attachment:undefined;border-width:1px;border-style:solid;border-color:transparent;width:410px;height:570px;overflow:hidden;'><div class='innerText'><p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p><p style="margin-top: 0px; margin-left: 0px;"><span style="font-size: 36px;">Goal of our Project</span></p><p><span style="color: rgb(255, 255, 255); line-height: 1.2; font-family: arial, arialregular, helvetica, sans-serif; font-size: 16px;">&nbsp;</span></p><p style="text-align: center; margin-top: 0px; margin-left: 0px;">Our circuit was designed to utilize specific parts so that when the Ecoli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.&nbsp;&nbsp;</p><p style="text-align: center; margin-top: 0px; margin-left: 0px;"><span style="font-size: 36px;">&nbsp;Testing the Circuit </span></p><p style="text-align: center; margin-top: 0px; margin-left: 0px;">• Before running the actual circuit, we ran control experiments to determine whether or not the GFP would indeed function as a reporter, meaning it can be regulated by a riboswitch.</p><p style="text-align: center; margin-top: 0px; margin-left: 0px;">• The first control experiment was constitutive: meaning that the GFP would always be produced. This was essentially only used to ensure that the GFP works in the paper ticket.</p><p style="text-align: center; margin-top: 0px; margin-left: 0px;">• The second control experiment was regulated via riboswitch SWH meaning that the GFP would be triggered. This tested if the GFP could be controlled/ regulated. • The results from both of these experiments are shown in the pictures below</p></div>
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<div class='ParagraphBox '  style='position:absolute;left:385px;top:1019px;z-index:10006;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;width:315px;height:260px;overflow:hidden;border-width:0px;border-style:solid;border-color:transparent;border-color:#000000;-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:303px;height:260px;overflow:hidden;'><div class='innerText'><p><span style="font-family: arial,arialregular,helvetica,sans-serif;"><span style="font-size: 24px;"><span style="line-height: 180%; background-color: transparent;">Ticket after first control experiment was run</span></span></span></p><p>&nbsp;</p><p><span style="color: rgb(105, 105, 105); line-height: 1.2; font-family: arial, ArialRegular, helvetica, sans-serif; font-size: 14px; background-color: transparent;">This experiment was the Constitutive GFP. The GFP was placed in the far left column, and only water was placed in the column next to it so that a control was present. BY comparing the far left column to the collum next to it, one can see the quantifiable difference between the wells with and without GFP</span></p><div>&nbsp;</div><p>&nbsp;</p><p>&nbsp;</p><p>&nbsp;</p><p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p><p>&nbsp;</p><p>&nbsp;</p><p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p><p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p><p>&nbsp;</p></div>
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<p class="MsoNormal"><span style="font-size:24px;"><span style="line-height: 115%; font-family: Verdana, sans-serif;">Our circuit was designed to utilize specific parts so that when the Ecoli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.  </span></span><o:p></o:p></p>
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<div class='ParagraphBox '  style='position:absolute;left:9px;top:1019px;z-index:10004;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;width:310px;height:251px;overflow:hidden;border-width:0px;border-style:solid;border-color:transparent;border-color:#000000;-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:298px;height:251px;overflow:hidden;'><div class='innerText'><p><span style="font-family: arial,arialregular,helvetica,sans-serif;"><span style="font-size: 24px;"><span style="line-height: 180%; background-color: transparent;">The original ticket before running</span></span></span></p><p>&nbsp;</p><p><span style="color: rgb(105, 105, 105);"><span style="font-family: arial, ArialRegular, helvetica, sans-serif; font-size: 14px;">Zen has a team of over 50 consultants, mostly with past experience in the energy industry. The team has deep knowledge of most aspects of the energy business, including particularly fuels and risk management.</span></span></p><p><span style="color: rgb(105, 105, 105);"><span style="font-family: arial, ArialRegular, helvetica, sans-serif; font-size: 14px;">&nbsp;</span></span></p><p>&nbsp;</p><p>&nbsp;</p><p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p><p>&nbsp;</p><p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p><p>&nbsp;</p><p>&nbsp;</p><p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p><p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p><p>&nbsp;</p></div>
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to determine whether or not the GFP would indeed function as a reporter,
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• The first control experiment was constitutive: meaning
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<br> • The second control experiment was regulated via riboswitch
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h2 id="vbid-559f00b3-bwglemsl" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >The original ticket before running</h2>
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<br class="lower-line-break" />
  
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<p>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<p style="line-height: 1.5em; font-size: 13px; font-family: Verdana, Arial, Helvetica, sans-serif; overflow: visible; background-color: rgb(224, 224, 224);"><span style="font-size:18px;"><span style="height: auto; width: auto; position: relative; color: rgb(105, 105, 105);"><span style="height: auto; width: auto; position: relative; font-family: arial, ArialRegular, helvetica, sans-serif;">Zen has a team of over 50 consultants, mostly with past experience in the energy industry. The team has deep knowledge of most aspects of the energy business, including particularly fuels and risk management.</span></span></span></p><p style="line-height: 1.5em; font-size: 13px; font-family: Verdana, Arial, Helvetica, sans-serif; overflow: visible; background-color: rgb(224, 224, 224);"><span style="height: auto; width: auto; position: relative; color: rgb(105, 105, 105);"><span style="height: auto; width: auto; position: relative; font-family: arial, ArialRegular, helvetica, sans-serif; font-size: 14px;"> </span></span></p>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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<p style="line-height: 1.5em; font-size: 13px; font-family: Verdana, Arial, Helvetica, sans-serif; overflow: visible; background-color: rgb(224, 224, 224);"><span style="font-size:18px;"><span style="height: auto; width: auto; position: relative; color: rgb(105, 105, 105); line-height: 1.2; font-family: arial, ArialRegular, helvetica, sans-serif; background-color: transparent;">This experiment was the Constitutive GFP. The GFP was placed in the far left column, and only water was placed in the column next to it so that a control was present. BY comparing the far left column to the collum next to it, one can see the quantifiable difference between the wells with and without GFP</span></span></p><div><br></div>
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Revision as of 23:38, 19 June 2017

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Cadets2Vets

Demonstrate - CADETS2VETS

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Goal of Our Project



Our circuit was designed to utilize specific parts so that when the Ecoli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.


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Testing the circuit

• Before running the actual circuit, we ran control experiments to determine whether or not the GFP would indeed function as a reporter, meaning it can be regulated by a riboswitch
. • The first control experiment was constitutive: meaning that the GFP would always be produced. This was essentially only used to ensure that the GFP works in the paper ticket.
• The second control experiment was regulated via riboswitch SWH meaning that the GFP would be triggered. This tested if the GFP could be controlled/ regulated.
• The results from both of these experiments are shown in the pictures below



The original ticket before running



Zen has a team of over 50 consultants, mostly with past experience in the energy industry. The team has deep knowledge of most aspects of the energy business, including particularly fuels and risk management.



Ticket after first control experiment



This experiment was the Constitutive GFP. The GFP was placed in the far left column, and only water was placed in the column next to it so that a control was present. BY comparing the far left column to the collum next to it, one can see the quantifiable difference between the wells with and without GFP




Ticket after running second experiment



This experiments used the same controls in order to compare the GFP column to the control (water) column.


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