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<img src="https://static.igem.org/mediawiki/2017/7/72/T--ETH_Zurich--ANDgateA.png" alt="FIXME"> | <img src="https://static.igem.org/mediawiki/2017/7/72/T--ETH_Zurich--ANDgateA.png" alt="FIXME"> | ||
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Revision as of 00:46, 28 October 2017
![CATE logo](https://static.igem.org/mediawiki/2017/d/d6/T--ETH_Zurich--LogoMinimal.png)
Parts
![FIXME](https://static.igem.org/mediawiki/2017/7/7e/T--ETH_Zurich--basicparts.png)
Overview
We submitted in total 14 new BioBricks to the iGEM registry! You can find all parts and their design below.
Basic Parts
Part | Description | BioBrick |
---|---|---|
Bacterioferritin | Heme-deletion mutant of bacterial iron storage protein functioning as MRI contrast agent | BBa_K2500000 |
Azurin | Mature cytotoxic copper protein | BBa_K2500001 |
p28 | Effector domain of Azurin | BBa_K2500002 |
PtlpA | Best Basic Part: Temperature-responsive promoter TlpA optimized for slight activation above 37°C and full activation at 45 °C | BBa_K2500003 |
TlpA | Protein reversibly inhibiting the pTlpA promoter at temperatures below 37 °C | BBa_K2500004 |
AND gate a | Synthetic promoter responsive to LldR and luxR | BBa_K2500010 |
AND gate b | Synthetic promoter responsive to LldR and luxR | BBa_K2500011 |
AND gate c | Synthetic promoter responsive to LldR and luxR | BBa_K2500012 |
Composite Parts
Part | Description | BioBrick |
---|---|---|
Pconst_RBSeng_TlpA | Best Composite Part:Temperature-dependent transcriptional repressor TlpA regulated by constant promoter and engineered RBS | BBa_K2500008 |
pTlpA_Protein E | Bacterial cell lysing protein E regulated by heat-inducible promoter pTlpA and synthetic RBS | BBa_K2500009 |
Pconst_RBS_LldP/ LldR_Pconst_RBS_LuxR |
Expression cassette consisting of LuxR and LldP/LldR | BBa_K2500013 |
Design
BBa_K2500010: AND gate a
![FIXME](https://static.igem.org/mediawiki/2017/7/72/T--ETH_Zurich--ANDgateA.png)
In absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the binding sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcription. In design a, the distances between the intercalated promoter and the binding sites were adapted from BBa_K1847007.
Registry: BBa_K2500010
BBa_K2500011: AND gate b
![FIXME](https://static.igem.org/mediawiki/2017/6/6a/T--ETH_Zurich--ANDgateB.png)
In design b, each binding site was duplicated in order to achieve a potential zipper mechanism and stronger inhibition due to binding more LldR inhibitor proteins.
Registry: BBa_K2500011
BBa_K2500012: AND gate c
![FIXME](https://static.igem.org/mediawiki/2017/c/c2/T--ETH_Zurich--ANDgateC.png)
In design c, an artificial spacer was embedded between the pLux promoter and the O2 binding site in order to influence the looping dynamics.
Registry: BBa_K2500012