Difference between revisions of "Team:Munich/Protocols"

Revision as of 06:19, 28 October 2017

Protocols
For our project we used different methods which are described in respective protocols. We also included a detailed list of all chemicals, materials and the aim of each experiment. This page is for everybody who wants to reproduce our work or wants to get an overview, but also to share our experiences with the iGEM community.
General Cas13a Read-out Target Other Agarose gel electrophoresis PCR Miniprep Restriction digest Ligation Glycerol stock Protein purification with Ni-NTA Protein purification with Akta SDS page RNAse activity protocol Lyophilization Protein expression Phenol-chlorophorm purification of RNA Phenol-chlorophorm purification of DNA Urea gel for RNA detection In vitro transcription RNA purification with Norgen Kit FINA method for RNA extraction FINA method for DNA extraction First strand cDNA syntesis (NEB) RPA RPA on paper RPA TX Chemical transformation Electro-transformation Chemically competent cells Electro-competent cells Dephosphorylation Golden Gate Gibson PCR cleanup Sequencing sample prep Soft litography AuNP preparation AuNP-DNA conjugation AuNP linkage AuNP cleavage
Primers and sequences
General Cas13a Read-out Target Other

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 <font size=7 color=#787878><b>Primers and sequences</b></font>
 </td></tr>
+<tr class="lastRow"><td colspan=3 align=center valign=center>
+     <div class="month">
+<input type="checkbox" id="General" name="type"/><label for="General">General</label>
+		<input type="checkbox" id="Cas13a" name="type"/><label for="Cas13a">Cas13a</label>
+		<input type="checkbox" id="Readout" name="type"/> <label for="Readout">Read-out</label>
+		<input type="checkbox" id="Target" name="type"/> <label for="Target">Target</label>
+		<input type="checkbox" id="Other" name="type"/> <label for="Other">Other</label>
+</td>
+</tr>
 <tr><td class="no-padding" colspan=2 align=right valign=center height=10>
 <br><br><br><center><hr></center>