Difference between revisions of "Team:BOKU-Vienna/Notebook"
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<a href="#iqiuoy" id="Week 4" class="arrows" onclick="expand_collapse('4');"><pre><font size="+2"><b>Week 4</b></font> (24/07-28/07)</pre></a><span style="font-size:150%;"><b> </b></span> | <a href="#iqiuoy" id="Week 4" class="arrows" onclick="expand_collapse('4');"><pre><font size="+2"><b>Week 4</b></font> (24/07-28/07)</pre></a><span style="font-size:150%;"><b> </b></span> | ||
| − | <div id="4" class="yolo" style="display:none;"><p> | + | <div id="4" class="yolo" style="display:none;"> |
| + | <p> | ||
| + | <br>•PCR: 9, 10, 16, 29, 30, 31, 32, 33, 34, 35, 36, 41, 42, 43</br> | ||
| + | Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification. | ||
| + | <br><br>•Golden Gate Assembly: BB1_16, BB1_17, BB1_18, BB1_20, BB1_21, BB1_22, BB1_23, BB1_24, BB1_25, BB2_17, BB2_23, BB2_24, BB3_01 (unsuccessful), BB3_02, , BB3_04, BB3_05, BB3_06 (doubtful), BB3_08</br> | ||
| + | GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br> | ||
| + | <br>•Yeast S266C made electro-competent</br> | ||
| + | <br>•Sent for Sequencing: BB1_16, BB1_17, BB2_18, BB3_02, BB3_05, CRISPR-PPP</br> | ||
| + | </p></div> | ||
| + | |||
<a href="#kskjdh" id="Week 5" class="arrows" onclick="expand_collapse('5');"><pre><font size="+2"><b>Week 5</b></font> (31/07-04/08)</pre></a><span style="font-size:150%;"><b> </b></span> | <a href="#kskjdh" id="Week 5" class="arrows" onclick="expand_collapse('5');"><pre><font size="+2"><b>Week 5</b></font> (31/07-04/08)</pre></a><span style="font-size:150%;"><b> </b></span> | ||
| − | <div id="5" class="yolo" style="display:none;"><p> | + | <div id="5" class="yolo" style="display:none;"> |
| + | <p> | ||
| + | <br>•</br> | ||
| + | <br>•</br> | ||
| + | <br>•</br> | ||
| + | <br>•</br> | ||
| + | <br>•</br> | ||
| + | </p></div> | ||
<a href="#jgcfgr" id="Week 6" class="arrows" onclick="expand_collapse('6');"><pre><font size="+2"><b>Week 6</b></font> (07/08-11/08)</pre></a><span style="font-size:150%;"><b> </b></span> | <a href="#jgcfgr" id="Week 6" class="arrows" onclick="expand_collapse('6');"><pre><font size="+2"><b>Week 6</b></font> (07/08-11/08)</pre></a><span style="font-size:150%;"><b> </b></span> | ||
Revision as of 19:57, 28 October 2017
Notebook
Notebook.
All protocols we used for the methods can be found here:
Protocol
Week 1 (03/07-07/07)
Week 2 (10/07-14/07)
Week 3 (17/07-21/07)
Week 4 (24/07-28/07)
Week 5 (31/07-04/08)
Week 6 (07/08-11/08)
Week 7 (14/08-18/08)
Week 8 (21/08-25/08)
Week 9 (28/08-01/09)
Week 10 (04/09-08/09)
Week 11 (11/09-15/09)
Week 12 (18/09-22/09)
Week 13 (25/09-29/09)
