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<h3><center>Vesicle toxicity considerations</h3></center>
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<p style="padding-top:2%; padding-right: 15%; padding-left:15%; font-size:14px;" class="big"> <br>.<font> Detoxification of these nano-sized vesicles is crucial since the outer membranes of gram negative bacteria are rich in LPS, which stimulates inflammatory responses. To decrease the toxicity of LPS, and make OMV delivery a safe delivery method, we would need to ensure that OMV administration is non-hazardous. Different approaches have shown promise to reduce the presence of LPS. Detergent-based approaches, although costly and laborious, have proven effective for vaccine administration. More elegant approaches based on genetic modification have recently been explored. For example, the mutation from a hexa to a penta-acylated lipid A lead to the formation of less toxic OMVs, while retaining their key structural features.</p>
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Revision as of 08:38, 1 November 2017

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Safety and risk assessment

Vesicle detoxification approaches


. Detoxification of these nano-sized vesicles is crucial since the outer membranes of gram negative bacteria are rich in LPS, which stimulates inflammatory responses. To decrease the toxicity of LPS, and make OMV delivery a safe delivery method, we would need to ensure that OMV administration is non-hazardous. Different approaches have shown promise to reduce the presence of LPS. Detergent-based approaches, although costly and laborious, have proven effective for vaccine administration. More elegant approaches based on genetic modification have recently been explored. For example, the mutation from a hexa to a penta-acylated lipid A lead to the formation of less toxic OMVs, while retaining their key structural features.