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− | <p><b>3. Model your project:</b> The | + | <p><b>3. Model your project:</b> Our project was based with a biofactory in mind, rather than just to show that we could use <i> Nicotiana benthamiana </i> to create a therapeutic. To demonstrate this, we created a <a href="https://2017.igem.org/Team:Cardiff_Wales/Modelling">model </a>that can be used as a tool to calculate how many plants would be required to produce a single effective dose of therapeutic for a patient with a specified disease severity. The equation was developed to be flexible, so that any variable can be changed to suit new requirements and fulfil a different objective. For our project, we modelled how many plants we would need to create one effective dose of TSH-antagonist for one patient, with both severe and less severe Graves' disease. We demonstrated that the variables can be changed, by changing one of the variables ourselves. We showed that different plants used as expression vectors or using different expression systems, such as Potato Virus X or HyperTrans, resulted in different numbers of plants being required for a single effective dose. This just changed one variable, the 'plant section', but the therapeutic could change just as easily. As we had a biofactory in mind, we used information gained from our <a href="https://2017.igem.org/Team:Cardiff_Wales/Human_Practices"> Integrated Human Practices </a> to show that plants can be scaled up easily and linearly to create a biofactory. Using our model, and the human practices information, we calculated how many plants (of different species, cultivars, and expression systems) would be needed to give a single effective dose to every sufferer of Graves' disease in the US, assuming a mean severity of the disease. This can be seen on our <a href="https://2017.igem.org/Team:Cardiff_Wales/Modelling"> modelling </a> page. |
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Revision as of 12:17, 29 October 2017