Difference between revisions of "Team:Cardiff Wales/results"

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<p> Team luficerase created the <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404013"> 35S:Luc+:NosT construct </a>, and quantified the 35S promoter using a luciferase reporter assay.<br><br></p>
 
<p> Team luficerase created the <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404013"> 35S:Luc+:NosT construct </a>, and quantified the 35S promoter using a luciferase reporter assay.<br><br></p>
 
<img src="https://static.igem.org/mediawiki/2017/6/6f/T--Cardiff_Wales--35S-Luc%2Bgel.jpg"/><br><br>
 
<img src="https://static.igem.org/mediawiki/2017/6/6f/T--Cardiff_Wales--35S-Luc%2Bgel.jpg"/><br><br>
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<p> This photo shows the DNA gel that showed the level 1 reaction to create the <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404013"> 35S:Luc+:NosT construct </a> had worked. The DNA solution that this was taken from was later sequenced to confirm that the construct was correct. </p>
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<p> This photo shows the DNA gel that showed the level 1 reaction to create the <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404013"> 35S:Luc+:NosT construct </a> had worked. The DNA solution that this was taken from was later sequenced to confirm that the construct was correct.<br><br>
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<img src="https://static.igem.org/mediawiki/2017/f/f2/T--Cardiff_Wales--LUC-plate.png"/><br><br>
 
<img src="https://static.igem.org/mediawiki/2017/f/f2/T--Cardiff_Wales--LUC-plate.png"/><br><br>
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<p> This image shows leaf discs taken from plants infiltrated with <i> Agrobacterium </i> containing the 35S:Luc+:NosT construct. The red regions show high levels of luciferase expresion.<br><br></p>
 
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Revision as of 13:04, 29 October 2017




Our Results


Provide links to part pages grouped around different projects. luc plate, gel, tobacco picture



During our project, we had three smaller teams within our main team. Each of these teams had different but interlinked objectives, and consequently resulted in different sets of results. The results of each team are shown with descriptions on this page.




Team Luciferase



Team luficerase created the 35S:Luc+:NosT construct , and quantified the 35S promoter using a luciferase reporter assay.



This photo shows the DNA gel that showed the level 1 reaction to create the 35S:Luc+:NosT construct had worked. The DNA solution that this was taken from was later sequenced to confirm that the construct was correct.



This image shows leaf discs taken from plants infiltrated with Agrobacterium containing the 35S:Luc+:NosT construct. The red regions show high levels of luciferase expresion.