Difference between revisions of "Team:BOKU-Vienna/Notebook"

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<br>• PCR: #1, #2, #3, #4, #6, #15, #18, #20, #23, #24, #25, #26</br>
 
<br>• PCR: #1, #2, #3, #4, #6, #15, #18, #20, #23, #24, #25, #26</br>
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
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<br><br>• Golden Gate Assembly: BB1_09, BB1_10, BB1_11, BB1_12, BB1_13, BB2_01 (unsuccessful), BB2_02, BB2_03, BB2_04, BB2_05 (unsuccessful)</br>
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<br><br>• Golden Gate Assembly: <a href="#BB1_09" class="bgscroll"> BB1_09</a>, <a href="#BB1_10" class="bgscroll"> BB1_10</a>, <a href="#BB1_11" class="bgscroll"> BB1_11</a>, <a href="#BB1_12" class="bgscroll"> BB1_12</a>, <a href="#BB1_13" class="bgscroll"> BB1_13</a>, BB2_01 (unsuccessful), <a href="#BB2_02" class="bgscroll"> BB2_02</a>, <a href="#BB2_03" class="bgscroll"> BB2_03</a>, <a href="#BB2_04" class="bgscroll"> BB2_04</a>, BB2_05 (unsuccessful)</br>
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GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• pSIM5 pre-cultures (DH5alpha + BL21(DE3))</br>
 
<br>• pSIM5 pre-cultures (DH5alpha + BL21(DE3))</br>
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Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
 
<br><br>• Golden Gate Assembly: BB1_16, BB1_17, BB1_18, BB1_20, BB1_21, BB1_22, BB1_23, BB1_24, BB1_25, BB2_17, BB2_23, BB2_24, BB3_01 (unsuccessful), BB3_02, , BB3_04, BB3_05, BB3_06 (doubtful), BB3_08</br>
 
<br><br>• Golden Gate Assembly: BB1_16, BB1_17, BB1_18, BB1_20, BB1_21, BB1_22, BB1_23, BB1_24, BB1_25, BB2_17, BB2_23, BB2_24, BB3_01 (unsuccessful), BB3_02, , BB3_04, BB3_05, BB3_06 (doubtful), BB3_08</br>
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GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.</br>
 
<br>• Yeast S266C made electro-competent</br>
 
<br>• Yeast S266C made electro-competent</br>

Revision as of 15:23, 29 October 2017

Notebook

V

Notebook.


All protocols we used for the methods can be found here: Protocol

For further information regarding our Golden Gate Assembly products, please refer to our part collection site. 

Week 1 (03/07-07/07)
   
Week 2 (10/07-14/07)
   
Week 3 (17/07-21/07)
   
Week 4 (24/07-28/07)
   
Week 5 (31/07-04/08)
   
Week 6 (07/08-11/08)
   
Week 7 (14/08-18/08)
   
Week 8 (21/08-25/08)
   
Week 9 (28/08-01/09)
   
Week 10 (04/09-08/09)
   
Week 11 (11/09-15/09)
   
Week 12 (18/09-22/09)
   
Week 13 (25/09-29/09)