Difference between revisions of "Team:TAS Taipei/Collaborations"
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| − | We have continued and strengthened our long standing partnership with National Yang Ming University (NYMU_Taipei). | + | We have continued and strengthened our long-standing partnership with National Yang Ming University (NYMU_Taipei). They troubleshooted our cloning procedure and design of primers, gave us protocols for assaying biofilm production, and trained our future iGEMers (Leona and Catherine). <br><br> |
In return, we helped add to the characterization of their lactose-induced suicide mechanism: the Holin-Endolysin-NrtA system (<a href="http://parts.igem.org/Part:BBa_K2350021">BBa_K2350021</a>). We measured the population of their kill-switch bacteria in different lactose concentrations, and find that our results are consistent with their findings. | In return, we helped add to the characterization of their lactose-induced suicide mechanism: the Holin-Endolysin-NrtA system (<a href="http://parts.igem.org/Part:BBa_K2350021">BBa_K2350021</a>). We measured the population of their kill-switch bacteria in different lactose concentrations, and find that our results are consistent with their findings. | ||
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| − | We first met the CGU_Taiwan team at the end of our presentation for the Asia Pacific iGEM Conference hosted | + | We first met the CGU_Taiwan team at the end of our presentation for the Asia Pacific iGEM Conference hosted at National Chiao Tung University (NCTU). <br><br> |
| − | They were excited that our biofilms were able to trap nanoparticles and | + | They were excited that our biofilms were able to trap nanoparticles, and wondered if they could trap ink particles as well. CGU_Taiwan is working on improving the procedure to manufacture reprocessed paper. They found that a process called “flotation,” typically used to de-ink paper, could cause significant paper fiber loss. They wondered if biofilm could potentially replace the current flotation process to preserve paper fibers. We offered to test this for CGU_Taiwan. We used a similar experimental procedure as our preliminary biofilm trapping experiment. The results suggest that <b>our biofilm can trap ink particles</b>. As shown below, the relative absorbance of ink decreased by approximately 50% when mixed with biofilm. |
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<img src="https://static.igem.org/mediawiki/2017/7/70/T--TAS_Taipei--figure_3-18.jpg" alt="test" id="group"> | <img src="https://static.igem.org/mediawiki/2017/7/70/T--TAS_Taipei--figure_3-18.jpg" alt="test" id="group"> | ||
| − | <h4 class="subtitle"><b>A)</b> Our | + | <h4 class="subtitle"><b>A)</b> Our experimental results showed that <i>E. coli</i> overexpressing OmpR234 (BBa_K2229200) is producing more biofilm than a control which does not overexpress OmpR234 (BBa_K342003). <b>B)</b> CGU_Taiwan independently tested our constructs using crystal violet, a dye commonly used to quantify biofilm formation. BBa_K2229200 showed higher absorbance compared to the control BBa_K342003, reflecting the formation of more biofilm, which matches our results. |
<span class="subCred">Experiment: Yvonne Wei</span> | <span class="subCred">Experiment: Yvonne Wei</span> | ||
</h4> | </h4> | ||
Revision as of 12:01, 31 October 2017
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Project
Experiments
Modeling
Prototype
Human Practices
Safety
About Us
Attributions
Project
Experiment
Modeling
Prototype
Human Practice
Safety
About Us
Attributions
hi
COLLABORATIONS
NYMU_TAIPEI
We have continued and strengthened our long-standing partnership with National Yang Ming University (NYMU_Taipei). They troubleshooted our cloning procedure and design of primers, gave us protocols for assaying biofilm production, and trained our future iGEMers (Leona and Catherine).
In return, we helped add to the characterization of their lactose-induced suicide mechanism: the Holin-Endolysin-NrtA system (BBa_K2350021). We measured the population of their kill-switch bacteria in different lactose concentrations, and find that our results are consistent with their findings.
We independently tested the function of NYMU_Taipei’s lactose-induced kill-switch system. Different concentrations (0-250 mM) of lactose were tested for their effects on bacteria population. Experiment: Yvonne Wei
Additionally, this year two members from TAS (Catherine and Leona) are members on both NYMU and TAS teams. They learned the cloning cycle from TAS and helped run experiments and human practices for NYMU throughout the year. In Boston, they will also help NYMU present their project at the Giant Jamboree.
CGU_Taiwan
We first met the CGU_Taiwan team at the end of our presentation for the Asia Pacific iGEM Conference hosted at National Chiao Tung University (NCTU).
They were excited that our biofilms were able to trap nanoparticles, and wondered if they could trap ink particles as well. CGU_Taiwan is working on improving the procedure to manufacture reprocessed paper. They found that a process called “flotation,” typically used to de-ink paper, could cause significant paper fiber loss. They wondered if biofilm could potentially replace the current flotation process to preserve paper fibers. We offered to test this for CGU_Taiwan. We used a similar experimental procedure as our preliminary biofilm trapping experiment. The results suggest that our biofilm can trap ink particles. As shown below, the relative absorbance of ink decreased by approximately 50% when mixed with biofilm.
From left to right: control (biofilm only), control (ink only), biofilm mixed with 0.1x ink solution. More ink was pulled down when mixed with biofilm, and the color of the supernatant is clearer than the supernatant of the ink only group. Experiment: William Chen, Yvonne Wei
In return, CGU_Taiwan helped us independently verify that overexpression of OmpR234 (BBa_K2229200) produces more biofilms than control (BBa_K342003).
