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+ | <h1>Overview</h1> | ||
+ | All parts were codon-optimized for expression in <i>E. coli</i> Nissle with Geneious and modified to remove forbidden restriction sites. | ||
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Revision as of 12:07, 30 October 2017
Basic Parts
Overview
All parts were codon-optimized for expression in E. coli Nissle with Geneious and modified to remove forbidden restriction sites.Part | Description | BioBrick |
---|---|---|
Best Basic Part: AND Gate B |
Synthetic promoter responsive to LldR and luxR | BBa_K2500011 |
Bacterioferritin | Heme-deletion mutant of bacterial iron storage protein functioning as an MRI contrast agent | BBa_K2500000 |
Azurin | Redox protein originating from P. aeruginosa with anti-cancer activity | BBa_K2500001 |
p28 | Effector domain of azurin | BBa_K2500002 |
pTlpA | Temperature-responsive promoter optimized for slight activation above 37°C and full activation at 45 °C | BBa_K2500003 |
TlpA | Temperature-dependent transcriptional repressor of pTlpa | BBa_K2500004 |
RBS_TlpA | Temperature-dependent transcriptional repressor of pTlpa and synthetic RBS | BBa_K2500005 |
Protein E | Bacteria-lysing protein encoded by phage Phi X 17 | BBa_K2500006 |
RBSeng_TlpA | Temperature-dependent transcriptional repressor of pTlpa with engineered RBS | BBa_K2500007 |
RBSeng_ProteinE | Bacteria-lysing protein encoded by phage Phi X 174 with engineered RBS | BBa_K2500009 |
AND Gate A | Synthetic promoter responsive to LldR and luxR | BBa_K2500010 |
AND Gate C | Synthetic promoter responsive to LldR and luxR | BBa_K2500012 |
pConst_RBS_LldP/ LldR_pConst_RBS_LuxR |
Expression cassette consisting of LuxR and LldP/LldR | BBa_K2500013 |