Difference between revisions of "Team:Cardiff Wales/basicparts"

Line 5: Line 5:
 
<center>
 
<center>
 
<br><br><br>
 
<br><br><br>
−
<h1> Basic Parts </h1>
+
<h1> Basic Parts used in our Project </h1>
 
</center>
 
</center>
 
<br><br><br>
 
<br><br><br>
Line 61: Line 61:
 
     <td>WRKY30</td>
 
     <td>WRKY30</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td>An inducible promoter that responds to the presence of cellulose-derived oligomers</td>
+
     <td>An inducible promoter that responds to the presence of cellulose-derived oligomers<br><i>After submission to the registry we discovered that this promotor sequence was incorrect</i></td>
 
<td>507bp</td>
 
<td>507bp</td>
 
   </tr>
 
   </tr>
Line 68: Line 68:
 
     <td>Luc+</td>
 
     <td>Luc+</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td>A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate,
+
     <td>A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression. <i>Prior to submission to the registry we discovered that this promotor sequence was incorrect so it was not added. In order to generate the level 1 clones containing LUC+ (<a href="http://parts.igem.org/Part:BBa_K2404013">BBa_K2404013</a>, <a href="http://parts.igem.org/Part:BBa_K2404014">BBa_K240414</a>) we used a previously generated LUC+ that is contained within a different (not pSB1C3) level 0 plasmid</i> </td>
−
luciferin, which can be used to quantify gene expression </td>
+
 
<td>1653bp</td>
 
<td>1653bp</td>
 
   </tr>
 
   </tr>

Revision as of 12:51, 30 October 2017




Basic Parts used in our Project






Our team used several basic parts to create our gene constructs. Here is an overview of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.




Registry link Part Plasmid Summary Length
BBa_K2404000 TSH pSB1C3 A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does not have His-tags for purification 780bp
BBa_K2404001 TSHH pSB1C3 A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does have His-tags for purification 832bp
BBa_K2404002 PDF1.2 pSB1C3 An inducible promoter that responds to the presence of jasmonic acid in the cell 522bp
BBa_K2404003 PR2 pSB1C3 An inducible promoter that responds to the presence of salicylic acid in the cell 519bp
BBa_K2404004 GST6 pSB1C3 An inducible promoter that responds to the presence of salicylic acid in the cell 519bp
BBa_K2404005 WRKY30 pSB1C3 An inducible promoter that responds to the presence of cellulose-derived oligomers
After submission to the registry we discovered that this promotor sequence was incorrect		 507bp
BBa_K2404006 Luc+ pSB1C3 A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression. Prior to submission to the registry we discovered that this promotor sequence was incorrect so it was not added. In order to generate the level 1 clones containing LUC+ (BBa_K2404013, BBa_K240414) we used a previously generated LUC+ that is contained within a different (not pSB1C3) level 0 plasmid 1653bp
BBa_P10401 NosT pSB1C3 A terminator from the nopaline synthase gene which is native to the Agrobacterium tumor-inducing plasmid pTiT37 285bp
BBa_P10000 LexA pSB1C3 An estradiol inducible promoter from the LexA gene which is native to Escherichia coli 362bp
BBa_P10003 35S-OTMV pSB1C3 A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus 1446bp