Difference between revisions of "Team:XJTLU-CHINA/Demonstrate"

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             <h2>1. Determination of the minimal inhibitory concentration (MIC) on the three AMPs.</h2>
 
             <h2>1. Determination of the minimal inhibitory concentration (MIC) on the three AMPs.</h2>
 
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         <div class="para">
 
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             <p style="font-size:20px">
 
             <p style="font-size:20px">
 
                 Both single peptide of the three AMPs and the mixture of them have conspicuous lethal effect on S. aureus.
 
                 Both single peptide of the three AMPs and the mixture of them have conspicuous lethal effect on S. aureus.
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            <h2>2. Absorbance (cell density) of the biofilm of
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                <i>S. aureus</i> against different concentrations of the three individual AMPs or a mixture of them </h2>
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                <img width="650px" src="https://static.igem.org/mediawiki/2017/b/ba/Demonstration_5.png">
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            </figure>
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                <img width="650px" src="https://static.igem.org/mediawiki/2017/4/4e/Demonstration_6.png">
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            </figure>
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            <figure>
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                <img width="650px" src="https://static.igem.org/mediawiki/2017/3/3d/Demonstration_7.png">
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                <img width="650px" src="https://static.igem.org/mediawiki/2017/1/17/Demonstration_8.png">
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        <div class="para">
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            <p style="font-size:20px">The biofilm formation of S. aureus was to a great extent inhibited by these three AMPs.</p>
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        </div>
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        <div class="para">
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            <h2>3. Expression of LL-37 in bacteria by dot blotting</h2>
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        </div>
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        <div class="fig" align="center">
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            <figure>
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                <img width="650px" src="https://static.igem.org/mediawiki/2017/7/7a/Demonstration_9.png">
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            </figure>
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        <div class="para">
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            <h1>Experience and conclusions:</h1>
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            <ul>
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                <li>Peptides has shown their antimicrobial activity on
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                    <i>S. aureus</i>. And they can successfully expressed in
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                    <i>E. coli. </i>
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                </li>
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                <li>
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                    AgrA, which is an essential protein in quorum sensing construct, signifies toxicity to cells during growth.
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                </li>
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                <li>
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                    We have trouble in obtaining enough plasmid DNA extracted from
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                    <i>L. lactis </i>after transformation, probably due to either low copy number or low yield from the extraction
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                    protocol.
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                </li>
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            </ul>
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        </div>
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    <div class="text-center" style="background:#cfe5d9;">
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            <h1 style="font-weight:bolder;color:#006934; ">Collaborators and Supporters</h1>
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            <div class="container-fluid supporters-logos">
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                    <a href="http://www.synbio-tech.com.cn"><img class="" src="https://static.igem.org/mediawiki/2017/3/38/Synbio_tech_logo.png"></a>
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                </div>
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                    <a href="http://www.wx2h.com/web/index.php"><img src="https://static.igem.org/mediawiki/2017/f/f7/Wuxi_no2_hospital_logo.png"></a>
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                    <a href="http://www.chinapeptides.qianyan.biz"><img src="https://static.igem.org/mediawiki/2017/7/7d/Qiangyao_logo.png"></a>
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                </div>
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                    <a href="https://www.neb.com"><img class="" src="https://static.igem.org/mediawiki/2017/0/06/NEB_logo.png"></a>
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                    <a href="https://www.snapgene.com"><img src="https://static.igem.org/mediawiki/2017/c/cb/Snapgene_logo.png"></a>
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                    <a href="http://www.genscript.com"><img src="https://static.igem.org/mediawiki/2017/9/9b/Genscript.png"></a>
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                          <h4>Location</h4>
 +
                          <p  style="text-align:center;">Rm 363, Science Building<br>
 +
                          Xi'an Jiaotong-Liverpool University<br>
 +
                          111 Ren'ai Road, Suzhou, China<br>
 +
                          215123
 +
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                          <h4>Get in touch</h4>
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                          <img src="https://static.igem.org/mediawiki/2017/1/19/XJTLU_email.png" alt="emali" width=30 height=30>
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                          <p style="text-align:center;">igem@xjtlu.edu.cn</p>
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            <div class="text-center" style="background:#003b73;">
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                    <p style="text-align:center; color:white;">XJTLU-CHINA iGEM 2017</p>
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Revision as of 11:53, 30 October 2017

Desmonstration

Demonstration

Aimed at examining the efficacy of the antimicrobial peptides on killing S. aureus (each experiment below were repeated 3 times).

1. Determination of the minimal inhibitory concentration (MIC) on the three AMPs.

Both single peptide of the three AMPs and the mixture of them have conspicuous lethal effect on S. aureus.

2. Absorbance (cell density) of the biofilm of S. aureus against different concentrations of the three individual AMPs or a mixture of them

The biofilm formation of S. aureus was to a great extent inhibited by these three AMPs.

3. Expression of LL-37 in bacteria by dot blotting

Experience and conclusions:

  • Peptides has shown their antimicrobial activity on S. aureus. And they can successfully expressed in E. coli.
  • AgrA, which is an essential protein in quorum sensing construct, signifies toxicity to cells during growth.
  • We have trouble in obtaining enough plasmid DNA extracted from L. lactis after transformation, probably due to either low copy number or low yield from the extraction protocol.

Collaborators and Supporters

Location

Rm 363, Science Building
Xi'an Jiaotong-Liverpool University
111 Ren'ai Road, Suzhou, China
215123

Get in touch

emali

igem@xjtlu.edu.cn

XJTLU-CHINA iGEM 2017