Difference between revisions of "Team:SDU CHINA/basic parts"

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<div class="title"><span class="STYLE10"><strong>Table of the Basic Parts</strong> we submitted to the BioBrick registry</span> </div>
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         <td valign="top" ><p class="STYLE7" >Part Number </p></td>
 
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<p><strong>Table 1：Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong></p>
 
<p><strong>Table 1：Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong></p>
 
<p><strong>TK plasmid in A549 cell line, after hTERT was inserted into pGL-3 vector.</strong></p>
 
<p><strong>TK plasmid in A549 cell line, after hTERT was inserted into pGL-3 vector.</strong></p>
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<p><strong>Figure 1: The express efficiency of hTERT promoter in A549 cell line.</strong></p>
 
<p><strong>Figure 1: The express efficiency of hTERT promoter in A549 cell line.</strong></p>
 
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   <p><strong>Table 2: Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong>  </p>
 
   <p><strong>Table 2: Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong>  </p>
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   <p><strong>TK plasmid in A549 cell line, after survivin was inserted into pGL-3 vector.</strong></p>
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   <p><strong>TK plasmid in A549 cell line, after survivin was inserted into pGL-3 vector.</strong>
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<p><span class="STYLE8"><strong>Figure 2 :The express efficiency of survivin promoter in A549 cell line</strong></span>.</p>
 
<p><span class="STYLE8"><strong>Figure 2 :The express efficiency of survivin promoter in A549 cell line</strong></span>.</p>
 
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Revision as of 14:06, 30 October 2017






Table of the Basic Parts we submitted to the BioBrick registry


Part Number

Name

Type

Length

Description

BBa_K2321000

 hTERT promoter

Regulatory

809

tumor-specific promoter

BBa_K2321001

Survivin promoter

Regulatory

1024

tumor-specific promoter

BBa_K2321002

Luciferase

Reporter

1653

firefly luciferase reporter



Both hTERT and survivin are tumor-specific promoter, so the parameter of starting efficiency after transfection is most important. In order to explore the specificity and sensitivity of starting efficiency for each promoter, they were respectively inserted into double luciferase reporter vector. Then we selected two kinds of NSCLC cell lines, including A549， and measured the relative luciferase expression activity of the plasmids in different cell lines and different time points.



Table 1：Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on

TK plasmid in A549 cell line, after hTERT was inserted into pGL-3 vector.




Figure 1: The express efficiency of hTERT promoter in A549 cell line.




Table 2: Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on

TK plasmid in A549 cell line, after survivin was inserted into pGL-3 vector.




Figure 2 :The express efficiency of survivin promoter in A549 cell line.




For more details, please refer to the results.