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<h3 style="text-align:center"><br> Model to serve, Model to solve, Model for security</h3> | <h3 style="text-align:center"><br> Model to serve, Model to solve, Model for security</h3> | ||
− | <h4><br>Modeling, with its strictness and precision, can be an excellent tool for us to understand the disease and the biological process, and plays an important role in our project. To satisfy our <a href="https://2017.igem.org/Team:CPU_CHINA/description">experimental </a>requirements, we created a <a href="https://2017.igem.org/Team:CPU_CHINA/ | + | <h4><br>Modeling, with its strictness and precision, can be an excellent tool for us to understand the disease and the biological process, and plays an important role in our project. To satisfy our <a href="https://2017.igem.org/Team:CPU_CHINA/description">experimental </a>requirements, we created a <a href="https://2017.igem.org/Team:CPU_CHINA/rdmpdf">model of Th17/iTreg differentiation</a>. This model provides a new framework that can be used to analyze the dynamic characteristics of Th17/iTreg differentiation network. In addition, we created a <a href="https://2017.igem.org/Team:CPU_CHINA/ibcpdf">two-variable model</a> for the interactions between pro-inflammatory and anti-inflammatory cytokines by establishing ordinary differential equations (ODE). This model can be used to investigate the involvement of cytokines in the disease process. We finally explore the feasibility of our project by coupling the model with our project. Finally, aimed at security, we explored the relationships between our system and RA models. </h4> |
<div class="circle-row" id="row"> | <div class="circle-row" id="row"> | ||
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<h2>What did our model achieve?</h2> | <h2>What did our model achieve?</h2> | ||
<h4><br>We achieved 3 main aims in our modelling work</h4> | <h4><br>We achieved 3 main aims in our modelling work</h4> | ||
− | <h4><br>We presented a novel mathematical model of <a href="https://2017.igem.org/Team:CPU_CHINA/ | + | <h4><br>We presented a novel mathematical model of <a href="https://2017.igem.org/Team:CPU_CHINA/rdmpdf">TH17-iTreg differentiation</a> that reveals how the control system generates phenotypic diversity and how its final state can be regulated by various signals.</h4> |
− | <h4><br>We created a <a href="https://2017.igem.org/Team:CPU_CHINA/ | + | <h4><br>We created a <a href="https://2017.igem.org/Team:CPU_CHINA/ibcpdf">two-variable model</a> for the interactions between pro-inflammatory and anti-inflammatory cytokines, and demonstrateed that mathematical modeling can be used to investigate the involvement of cytokines in the disease process.</h4> |
<h4><br>We combined the two-variable model with our project to find the method of solving the kinetic parameters of the system, and the relationship between parameters and <a href="https://2017.igem.org/Team:CPU_CHINA/exppdf">system security.</a></h4> | <h4><br>We combined the two-variable model with our project to find the method of solving the kinetic parameters of the system, and the relationship between parameters and <a href="https://2017.igem.org/Team:CPU_CHINA/exppdf">system security.</a></h4> | ||
<h4><br>All of our models are available on our <a href="https://github.com/cbqcpu/iGEM_CPU_CHINA" >Github page.</a></h4> | <h4><br>All of our models are available on our <a href="https://github.com/cbqcpu/iGEM_CPU_CHINA" >Github page.</a></h4> | ||
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<tr> | <tr> | ||
<td align="center">A mathematical model for the reciprocal differentiation of T helper 17 cells and induced regulatory T cells. | <td align="center">A mathematical model for the reciprocal differentiation of T helper 17 cells and induced regulatory T cells. | ||
− | <br><a class="more" href="https://2017.igem.org/Team:CPU_CHINA/ | + | <br><a class="more" href="https://2017.igem.org/Team:CPU_CHINA/rdmpdf">READ MORE</a></td> |
− | <td align="center">A two-variable model for the interactions between pro-inflammatory and anti-inflammatory cytokines in rheumatoid arthritis.<br><a class="more" href="https://2017.igem.org/Team:CPU_CHINA/ | + | <td align="center">A two-variable model for the interactions between pro-inflammatory and anti-inflammatory cytokines in rheumatoid arthritis.<br><a class="more" href="https://2017.igem.org/Team:CPU_CHINA/ibcpdf">READ MORE</a></td> |
<td align="center">A mathematical representation of our SynNotch CAR-Tregs system and exploration of the previous two models | <td align="center">A mathematical representation of our SynNotch CAR-Tregs system and exploration of the previous two models | ||
<br><a class="more" href="https://2017.igem.org/Team:CPU_CHINA/exppdf">READ MORE</a></td> | <br><a class="more" href="https://2017.igem.org/Team:CPU_CHINA/exppdf">READ MORE</a></td> | ||
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</div> | </div> | ||
<h4 align=middle>Figure1. the different subsets of naive T cells induced by different cytokines</h4> | <h4 align=middle>Figure1. the different subsets of naive T cells induced by different cytokines</h4> | ||
− | <h4><br>Based on previous work, we know that IL-6 and TGF-β of certain concentrations can induce naïve T cells to differentiate into Th-17 cells. And this protocol has existed. But why is this appropriate concentration able to induce its successful differentiation? To solve this problem, we created a <a href="https://2017.igem.org/Team:CPU_CHINA/ | + | <h4><br>Based on previous work, we know that IL-6 and TGF-β of certain concentrations can induce naïve T cells to differentiate into Th-17 cells. And this protocol has existed. But why is this appropriate concentration able to induce its successful differentiation? To solve this problem, we created a <a href="https://2017.igem.org/Team:CPU_CHINA/rdmpdf">model of Th17/iTreg differentiation. READ MORE</a></h4> |
<div> | <div> | ||
<center><img src="https://static.igem.org/mediawiki/2017/0/09/T--CPU_CHINA--model_image002.png" width = "700"></center> | <center><img src="https://static.igem.org/mediawiki/2017/0/09/T--CPU_CHINA--model_image002.png" width = "700"></center> | ||
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<h4><br>The interaction between cytokines plays a decisive role in the development and progression of RA (Figure 2). And Inflammation is associated with imbalance in cytokines in the environment. To solve this problem, we establish Syn-Notch-CAR-Treg system which shows its ability to attenuate RA symptoms with the help of CAR and Syn-Notch. In the light of the macroscopic results, this system achieves its functions by changing the balance of cytokines in the environment: pro-inflammatory cytokines can simultaneously induce the production of anti-inflammatory cytokines and pro-inflammatory cytokines, while anti-inflammatory cytokines lead to a decrease in pro-inflammatory cytokines (Figure 3). Based on that, we have created a two-variable model for the interactions between pro-inflammatory and anti-inflammatory cytokines. We have also obtained some instructive results by studying the dynamic characteristics of the model (stability, oscillation, etc.). | <h4><br>The interaction between cytokines plays a decisive role in the development and progression of RA (Figure 2). And Inflammation is associated with imbalance in cytokines in the environment. To solve this problem, we establish Syn-Notch-CAR-Treg system which shows its ability to attenuate RA symptoms with the help of CAR and Syn-Notch. In the light of the macroscopic results, this system achieves its functions by changing the balance of cytokines in the environment: pro-inflammatory cytokines can simultaneously induce the production of anti-inflammatory cytokines and pro-inflammatory cytokines, while anti-inflammatory cytokines lead to a decrease in pro-inflammatory cytokines (Figure 3). Based on that, we have created a two-variable model for the interactions between pro-inflammatory and anti-inflammatory cytokines. We have also obtained some instructive results by studying the dynamic characteristics of the model (stability, oscillation, etc.). | ||
<a | <a | ||
− | class="more" href="https://2017.igem.org/Team:CPU_CHINA/ | + | class="more" href="https://2017.igem.org/Team:CPU_CHINA/ibcpdf">READ MORE</a> |
</h4> | </h4> | ||
Latest revision as of 15:42, 31 October 2017
Introduction
What can modeling do for our project? After careful consideration, our team thinks that what modeling can do does not merely simulate and verify our system though it is important. We hope that with the agency of our modeling, we can find some solutions to the problems we have met or will meet during the process of our project. Thus, we establish “3S” as goals of modeling:
Model to serve, Model to solve, Model for security
Modeling, with its strictness and precision, can be an excellent tool for us to understand the disease and the biological process, and plays an important role in our project. To satisfy our experimental requirements, we created a model of Th17/iTreg differentiation. This model provides a new framework that can be used to analyze the dynamic characteristics of Th17/iTreg differentiation network. In addition, we created a two-variable model for the interactions between pro-inflammatory and anti-inflammatory cytokines by establishing ordinary differential equations (ODE). This model can be used to investigate the involvement of cytokines in the disease process. We finally explore the feasibility of our project by coupling the model with our project. Finally, aimed at security, we explored the relationships between our system and RA models.
What did our model achieve?
We achieved 3 main aims in our modelling work
We presented a novel mathematical model of TH17-iTreg differentiation that reveals how the control system generates phenotypic diversity and how its final state can be regulated by various signals.
We created a two-variable model for the interactions between pro-inflammatory and anti-inflammatory cytokines, and demonstrateed that mathematical modeling can be used to investigate the involvement of cytokines in the disease process.
We combined the two-variable model with our project to find the method of solving the kinetic parameters of the system, and the relationship between parameters and system security.
All of our models are available on our Github page.
Reciprocal Differentiation Model | Cytokine-mediated inflammation in RA | Exploration |
---|---|---|
A mathematical model for the reciprocal differentiation of T helper 17 cells and induced regulatory T cells.
READ MORE |
A two-variable model for the interactions between pro-inflammatory and anti-inflammatory cytokines in rheumatoid arthritis. READ MORE |
A mathematical representation of our SynNotch CAR-Tregs system and exploration of the previous two models
READ MORE |