Difference between revisions of "Team:TUDelft/Main-Parts"
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| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306000 target="_blank">BBa_K2306000</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>TorA-GFP fusion</td> | <td>TorA-GFP fusion</td> | ||
| Line 42: | Line 42: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306001 target="_blank">BBa_K2306001</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>TorA-GFP fusion (LacI promotor)</td> | <td>TorA-GFP fusion (LacI promotor)</td> | ||
| Line 50: | Line 50: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306002 target="_blank">BBa_K2306002</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Secretory-abundant heat soluble protein 68234 (SAHS 68234)</td> | <td>Secretory-abundant heat soluble protein 68234 (SAHS 68234)</td> | ||
| Line 58: | Line 58: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306003 target="_blank">BBa_K2306003</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Secretory-abundant heat soluble protein 33020 (SAHS 33020)</td> | <td>Secretory-abundant heat soluble protein 33020 (SAHS 33020)</td> | ||
| Line 66: | Line 66: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306005 target="_blank">BBa_K2306005</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Cytosolic-abundant heat soluble protein 106094 (CAHS 106094)</td> | <td>Cytosolic-abundant heat soluble protein 106094 (CAHS 106094)</td> | ||
| Line 74: | Line 74: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306006 target="_blank">BBa_K2306006</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Cytosolic-abundant heat soluble protein 94205 (CAHS 94205)</td> | <td>Cytosolic-abundant heat soluble protein 94205 (CAHS 94205)</td> | ||
| Line 82: | Line 82: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306007 target="_blank">BBa_K2306007</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Secretory-abundant heat soluble protein 68234 (SAHS 68234) (T7 promoter, RBS and double terminator)</td> | <td>Secretory-abundant heat soluble protein 68234 (SAHS 68234) (T7 promoter, RBS and double terminator)</td> | ||
| Line 90: | Line 90: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Secretory-abundant heat soluble protein 33020 (SAHS 33020) (T7 promoter, RBS and double terminator)</td> | <td>Secretory-abundant heat soluble protein 33020 (SAHS 33020) (T7 promoter, RBS and double terminator)</td> | ||
| Line 98: | Line 98: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306010 target="_blank">BBa_K2306010</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Cytosolic-abundant heat soluble protein 106094 (CAHS 106094) (T7 promoter, RBS and double terminator</td> | <td>Cytosolic-abundant heat soluble protein 106094 (CAHS 106094) (T7 promoter, RBS and double terminator</td> | ||
| Line 106: | Line 106: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306011 target="_blank">BBa_K2306011</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Cytosolic-abundant heat soluble protein 94205 (CAHS 94205) (T7 promoter, RBS and double terminator)</td> | <td>Cytosolic-abundant heat soluble protein 94205 (CAHS 94205) (T7 promoter, RBS and double terminator)</td> | ||
| Line 114: | Line 114: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306012 target="_blank">BBa_K2306012</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Cas13a </td> | <td>Cas13a </td> | ||
| Line 122: | Line 122: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306013 target="_blank">BBa_K2306013</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Cas13a spacer with flanking double repeats, constitutive promoter</td> | <td>Cas13a spacer with flanking double repeats, constitutive promoter</td> | ||
| Line 130: | Line 130: | ||
<tr> | <tr> | ||
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306015 target="_blank">BBa_K2306015</a></td> |
<td>Other</td> | <td>Other</td> | ||
<td>Cas13a spacer with flanking double repeats</td> | <td>Cas13a spacer with flanking double repeats</td> | ||
| Line 153: | Line 153: | ||
</thead> | </thead> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306002 target="_blank">BBa_K2306002</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 68234</td> | <td>Secretory-abundant heat soluble protein (SAHS) 68234</td> | ||
| Line 160: | Line 160: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306003 target="_blank">BBa_K2306003</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 33020</td> | <td>Secretory-abundant heat soluble protein (SAHS) 33020</td> | ||
| Line 167: | Line 167: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306005 target="_blank">BBa_K2306005</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Cytosolic-abundant heat soluble protein (CAHS) 106094</td> | <td>Cytosolic-abundant heat soluble protein (CAHS) 106094</td> | ||
| Line 174: | Line 174: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306006 target="_blank">BBa_K2306006</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Cytosolic-abundant heat soluble protein (CAHS) 94205</td> | <td>Cytosolic-abundant heat soluble protein (CAHS) 94205</td> | ||
| Line 181: | Line 181: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306000 target="_blank">BBa_K2300600</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>TolR GFP</td> | <td>TolR GFP</td> | ||
| Line 188: | Line 188: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306012 target="_blank">BBa_K2306012</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Cas13a</td> | <td>Cas13a</td> | ||
| Line 195: | Line 195: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306015 target="_blank">BBa_K2306015</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Spacer with flanking double repeats for Cas13a</td> | <td>Spacer with flanking double repeats for Cas13a</td> | ||
| Line 214: | Line 214: | ||
</thead> | </thead> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306007 target="_blank">BBa_K2306007</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promotor, RBS and double terminator</td> | <td>Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promotor, RBS and double terminator</td> | ||
| Line 221: | Line 221: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator</td> | <td>Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator</td> | ||
| Line 228: | Line 228: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306010 target="_blank">BBa_K2306010</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promotor, RBS and double terminator</td> | <td>Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promotor, RBS and double terminator</td> | ||
| Line 235: | Line 235: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306011 target="_blank">BBa_K2306011</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promotor, RBS and double terminator</td> | <td>Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promotor, RBS and double terminator</td> | ||
| Line 242: | Line 242: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306001 target="_blank">BBa_K2306001</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>TolR GFP fusion with inducible LacI promotor, RBS and double terminator</td> | <td>TolR GFP fusion with inducible LacI promotor, RBS and double terminator</td> | ||
| Line 249: | Line 249: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306015 target="_blank">BBa_K2306015</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Cas13a spacer with flanking double repeats, constitutive promoter</td> | <td>Cas13a spacer with flanking double repeats, constitutive promoter</td> | ||
| Line 269: | Line 269: | ||
</thead> | </thead> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306007 target="_blank">BBa_K2306007</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promotor, RBS and double terminator</td> | <td>Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promotor, RBS and double terminator</td> | ||
| Line 276: | Line 276: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator</td> | <td>Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator</td> | ||
| Line 283: | Line 283: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306010 target="_blank">BBa_K2306010</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promotor, RBS and double terminator</td> | <td>Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promotor, RBS and double terminator</td> | ||
| Line 290: | Line 290: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306011 target="_blank">BBa_K2306011</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promotor, RBS and double terminator</td> | <td>Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promotor, RBS and double terminator</td> | ||
Revision as of 22:47, 30 October 2017
Parts
When constructing our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts we synthesised contain the coding sequence, RBS, promoter and terminators, if applicable. Therefore, these parts are composite. The different basic parts comprising the composite parts are documented separately, and also exist as separate biobricks. This is advantageous for those wishing to combine one of our basic parts with a different expression level or compose a different composite part altogether. These basic parts were generated by using PCR and restriction ligation on the synthesised composite parts. We created a part collection comprised of 4 composite parts encoding for different Tardigrade Proteins (TDPs) and the 4 basic parts of these genes, all with elaborate characterisation so the user may choose the best protein for their project accordingly. For the composite parts of the TDPs we designed a new, strong RBS. This part does not exist separately. Additionally, we include the documentation of a basic part encoding the gene for Cas13a, derived from the plasmid pC011 from Gootenberg et al (2017). This biobrick does not exist separately. Finally, we submitted a composite part containing a nonsense spacer with flanking double repeats (with constitutive promoter), appropriate for use for Cas13a. This part is designed so that the user can easily change the spacer according to the sequence they wish to target. Find more info on this cool part on its page in the registry! We also documented this as a basic part without a promoter. This part does not exist separately.
In our project, we added to the characterisation of an existing Biobrick, the TolR, Periplasmic domain design by Gavin Sutton from iGEM Australia 2016. Additionally, our TolR-GFP composite part was based on the biobrick created by NTU Trontheim with some alterations, such as the removal of the RFP sequence. This enabled us to build on their knowledge and improve the function.
Improved BioBrick / Further Characterisation
All Parts
Name
Type
Description
Designer
Length
BBa_K2306000
Coding
TorA-GFP fusion
Gabriella Tany
846
BBa_K2306001
Composite
TorA-GFP fusion (LacI promotor)
Gabriella Tany
1212
BBa_K2306002
Coding
Secretory-abundant heat soluble protein 68234 (SAHS 68234)
Guillermo Serena Ruiz
525
BBa_K2306003
Coding
Secretory-abundant heat soluble protein 33020 (SAHS 33020)
Guillermo Serena Ruiz
509
BBa_K2306005
Coding
Cytosolic-abundant heat soluble protein 106094 (CAHS 106094)
Guillermo Serena Ruiz
684
BBa_K2306006
Coding
Cytosolic-abundant heat soluble protein 94205 (CAHS 94205)
Guillermo Serena Ruiz
681
BBa_K2306007
Composite
Secretory-abundant heat soluble protein 68234 (SAHS 68234) (T7 promoter, RBS and double terminator)
Guillermo Serena Ruiz
768
BBa_K2306008
Composite
Secretory-abundant heat soluble protein 33020 (SAHS 33020) (T7 promoter, RBS and double terminator)
Guillermo Serena Ruiz
752
BBa_K2306010
Composite
Cytosolic-abundant heat soluble protein 106094 (CAHS 106094) (T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
927
BBa_K2306011
Composite
Cytosolic-abundant heat soluble protein 94205 (CAHS 94205) (T7 promoter, RBS and double terminator)
Guillermo Serena Ruiz
924
BBa_K2306012
Coding
Cas13a
Jeroen Jacques
3920
BBa_K2306013
Composite
Cas13a spacer with flanking double repeats, constitutive promoter
Jeroen Jacques and Jasper Veerman
134
BBa_K2306015
Other
Cas13a spacer with flanking double repeats
Jeroen Jacques and Jasper Veerman
99
Basic Parts
Name
Type
Description
Designer
Size (bp)
BBa_K2306002
Basic
Secretory-abundant heat soluble protein (SAHS) 68234
Guillermo Serena Ruiz
525
BBa_K2306003
Basic
Secretory-abundant heat soluble protein (SAHS) 33020
Guillermo Serena Ruiz
509
BBa_K2306005
Basic
Cytosolic-abundant heat soluble protein (CAHS) 106094
Guillermo Serena Ruiz
684
BBa_K2306006
Basic
Cytosolic-abundant heat soluble protein (CAHS) 94205
Guillermo Serena Ruiz
681
BBa_K2300600
Basic
TolR GFP
Gabriella Tany
681
BBa_K2306012
Basic
Cas13a
Jeroen Jacques
3456
BBa_K2306015
Basic
Spacer with flanking double repeats for Cas13a
Jeroen Jacques, Jasper Veerman
99
Composite Parts
Name
Type
Description
Designer
Size (bp)
BBa_K2306007
Composite
Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
833
BBa_K2306008
Composite
Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
817
BBa_K2306010
Composite
Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
992
BBa_K2306011
Composite
Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
989
BBa_K2306001
Composite
TolR GFP fusion with inducible LacI promotor, RBS and double terminator
Gabriella Tany
681
BBa_K2306015
Composite
Cas13a spacer with flanking double repeats, constitutive promoter
Jeroen Jacques, Jasper Veerman
134
Part Collection
Name
Type
Description
Designer
Size (bp)
BBa_K2306007
Composite
Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
833
BBa_K2306008
Composite
Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
817
BBa_K2306010
Composite
Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
992
BBa_K2306011
Composite
Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
989
