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<h2> Our constructs </h2> | <h2> Our constructs </h2> | ||
− | + | ||
<div class="personal_presentation_flip"> | <div class="personal_presentation_flip"> | ||
<a href="https://static.igem.org/mediawiki/2017/b/ba/T--Linkoping_Sweden--superduperplasmid.PNG" title="Click to make the plasmid bigger!" target="_blank"> | <a href="https://static.igem.org/mediawiki/2017/b/ba/T--Linkoping_Sweden--superduperplasmid.PNG" title="Click to make the plasmid bigger!" target="_blank"> | ||
<img id="superplasmid"src="https://static.igem.org/mediawiki/2017/b/ba/T--Linkoping_Sweden--superduperplasmid.PNG"/> | <img id="superplasmid"src="https://static.igem.org/mediawiki/2017/b/ba/T--Linkoping_Sweden--superduperplasmid.PNG"/> | ||
</a> | </a> | ||
− | <p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and Trigger Factor. </p> | + | <h4> Chaperone systems </h4> <p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and Trigger Factor. </p> |
<p> We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid. The psb1C3 backbone contains a gene for chloramphenicol resistence. </p> | <p> We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid. The psb1C3 backbone contains a gene for chloramphenicol resistence. </p> | ||
Revision as of 12:59, 29 June 2017