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| | <p>Heavy metals (Cu2+, Cd2+, etc.) influence every aspect of human life. Although they are important to chemical, medical, and manufacturing industry, exposure to them has caused lots of hazards to humans.</p> | | <p>Heavy metals (Cu2+, Cd2+, etc.) influence every aspect of human life. Although they are important to chemical, medical, and manufacturing industry, exposure to them has caused lots of hazards to humans.</p> |
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| − | <p>With the rapid development of global industrialization, heavy metal pollution has become a severe problem threatening everyone’s health. Thanks to the global collaboration with more than 20 teams involved, our principals for human practice and model has completed a world map for some heavy metals’ pollution. The result illustrates a serious situation we are faced with.</p> | + | <p>With the rapid development of global industrialization, heavy metal pollution has become a severe problem threatening everyone's health. Thanks to the global collaboration with more than 20 teams involved, our principals for human practice and model has completed a world map for some heavy metals' pollution. The result illustrates a serious situation we are faced with.</p> |
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| | <img src="https://static.igem.org/mediawiki/2017/thumb/9/9a/Tianjin-cadmiumworldwideBLUE.jpg/1600px-Tianjin-cadmiumworldwideBLUE.jpg"> | | <img src="https://static.igem.org/mediawiki/2017/thumb/9/9a/Tianjin-cadmiumworldwideBLUE.jpg/1600px-Tianjin-cadmiumworldwideBLUE.jpg"> |
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| | <p>After actuality investigation with government, companies and NGOs, three key questions have been summed up. How can microorganism survive from a relatively high concentration of heavy metals? How can we separate different metals with fewer reactions? How can we monitor the water quality after treatment in real time?</p> | | <p>After actuality investigation with government, companies and NGOs, three key questions have been summed up. How can microorganism survive from a relatively high concentration of heavy metals? How can we separate different metals with fewer reactions? How can we monitor the water quality after treatment in real time?</p> |
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| − | <p>For these questions, we chose to use a brand-new strategy, synthetic life, to answer them. Within the framework of <a href="http://syntheticyeast.org/sc2-0/"> Sc2.0</a> project, scientists from all over the world work together to build the world’s first synthetic eukaryotic genome - <i>Saccharomyces cerevisiae</i>’s genome. Chinese team from Tianjin University has completed two synthetic chromosomes, and we are very lucky to use the synthetic yeast to solve these questions.</p> | + | <p>For these questions, we chose to use a brand-new strategy, synthetic life, to answer them. Within the framework of <a href="http://syntheticyeast.org/sc2-0/"> Sc2.0</a> project, scientists from all over the world work together to build the world's first synthetic eukaryotic genome - <i>Saccharomyces cerevisiae</i>'s genome. Chinese team from Tianjin University has completed two synthetic chromosomes, and we are very lucky to use the synthetic yeast to solve these questions.</p> |
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| − | <p>We used a particular genome evolution system, SCRaMbLE (Synthetic Chromosome Recombination and Modification by LoxP-mediated Evolution), to rapidly obtain the budding yeast with tolerance of high concentration of heavy metals. To answer the second question, we also boldly and creatively designed a orthogonal switcher with the SCRaMbLE system, based on the change of yeast’s mating type which determine the sexuality. We also improved and created many parts to build a biosensor for Cu ions with higher sensitivity and wider response range.</p> | + | <p>We used a particular genome evolution system, SCRaMbLE (Synthetic Chromosome Recombination and Modification by LoxP-mediated Evolution), to rapidly obtain the budding yeast with tolerance of high concentration of heavy metals. To answer the second question, we also boldly and creatively designed a orthogonal switcher with the SCRaMbLE system, based on the change of yeast's mating type which determine the sexuality. We also improved and created many parts to build a biosensor for Cu ions with higher sensitivity and wider response range.</p> |
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| − | <p> Let’s get started with <a href="https://2017.igem.org/Team:Tianjin/Design"> Design</a> and <a href="https://2017.igem.org/Team:Tianjin/Demonstrate"> Demonstrate</a>.</p> | + | <p> Let's get started with <a href="https://2017.igem.org/Team:Tianjin/Design"> Design</a> and <a href="https://2017.igem.org/Team:Tianjin/Demonstrate"> Demonstrate</a>.</p> |
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