Difference between revisions of "Team:U of Guelph/Notebook"
| Line 131: | Line 131: | ||
<p class="descP">Made LB broth and an agarose gel. | <p class="descP">Made LB broth and an agarose gel. | ||
</p> | </p> | ||
| − | <h1 class="nbDay"> | + | <h1 class="nbDay">July 12</h1> |
| − | <p class="descP"> | + | <p class="descP">Re Streaked BL21 (onto LB) and DH5α/pET-28a (onto LB Kan).</p> |
| − | <h1 class="nbDay"> | + | <h1 class="nbDay">July 13</h1> |
| − | <p class="descP"> | + | <p class="descP"> Overnight liquid cultures were prepared of BL21 and DH5α / pET-28a in 4 mL LB broth in plastic culture tubes. Kanamycin was forgotten in pET-28a culture. Ordered Primers for <i>frc</i> and <i>oxc</i> genes without RE end sites.</p> |
| − | <h1 class="nbDay"> | + | <h1 class="nbDay">July 14</h1> |
| − | <p class="descP"> | + | <p class="descP">Prepared BL21 Competent Cells, Miniprep of DH5α / pET-28a.</p> |
<h1 class="nbDay">Insert Day here</h1> | <h1 class="nbDay">Insert Day here</h1> | ||
<p class="descP"> Here include information on what happened this day</p> | <p class="descP"> Here include information on what happened this day</p> | ||
Revision as of 11:44, 31 October 2017
Notebook
Experiment Overview
Here we will include a day by day entry for our project. We need to make sure we atleast include the days we did which experiments but more information would be good too. Note who participated in what should be included.
June 5
LB and LB Kan Plates were poured.
June 6
Re-streaked Ecoli BL21, DH5α, and DH5α/ pET-28a from the stocks we were provided.
June 7
Liquid cultures of DH5α, DH5α/ pET-28a and BL21 were inoculated and grown for 48 hours
June 9
Glycerol stocks were prepared from the liquid cultures
June 14
Ordered frc and oxc Full length Sequences
June 19
Ordered gBlock fragments of frc and oxc for use in a gibson assembly
June 20
Ordered original PCR primers to add PstI to pET-28a
June 21
A half sleeve of each chloramphenicol and Kanamycin plates were poured, DH5α/pET-28a was re-streaked, and a DH5α liquid overnight culture was prepared.
June 22
DH5α competent cells were made. Experiment failed at the final step due to forgetting the glycerol before putting cells in liquid nitrogen.
June 25
Ordered primers to add frc and oxc ends to pET-28a(PstI).
July 5
Miniprep DH5α/pET-28a, and PCR to add PstI to pET-28a.
July 6
Gibson Assembly transformation.
July 10
Made LB broth and an agarose gel.
July 12
Re Streaked BL21 (onto LB) and DH5α/pET-28a (onto LB Kan).
July 13
Overnight liquid cultures were prepared of BL21 and DH5α / pET-28a in 4 mL LB broth in plastic culture tubes. Kanamycin was forgotten in pET-28a culture. Ordered Primers for frc and oxc genes without RE end sites.
July 14
Prepared BL21 Competent Cells, Miniprep of DH5α / pET-28a.
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Note to change last entry class
