Difference between revisions of "Team:UST Beijing/Experiments"

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           We used a composite part we constructed in 2016 iGEM competition (http://parts.igem.org/Part:BBa_K2072000:Design) for our following experiments. The plasmid pSB1C3/BBa_2072000 was transformed into E.coli BL21(DE3) cells and cultivated in 96-well microplates with following medium components: M9 salt mixture, chloramphenicol, glycerol and specific sugars, e.g. glucose, arabinose, or IPTG. PNPG was also included.  The culture was shaken in 37 degrees C for up to 20 hours. The plate was analyzed with a microplate reader with wavelength at 405 and 620 nm. The data was collected in spreadsheet and analyzed using Matlab.
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           In this year project,we have mainly done two things:firstly ,we tested the plasmid we designed in 2016IGEM program ,and we have found something interesting about ARA promoter。Besides,we have also found that we can use E.coli itself to hydrolyze pseudo-ginseng ,and it is safer than E.coli with plasmid we designed. And we think it fits our theme―food and nutation.
 
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               <h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Strain used</h4>
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Escherichia coli BL21(DE3) <br/>
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<h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Machines</h4>
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CLC Sequence Viewer 6<br/>
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Chimera 1.8<br/>
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NCBI<br/>
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Swiss Model<br/>
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              <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Methods</h4>
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<h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Protocols</h4>
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Please click the link:<br/>
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<a href="https://2017.igem.org/Competition/InterLab_Study">https://2017.igem.org/Competition/InterLab_Study</a>>
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Revision as of 14:26, 31 October 2017

USTB-Beijing | Welcome

PanGu Cyclase Overview


In this year project,we have mainly done two things:firstly ,we tested the plasmid we designed in 2016IGEM program ,and we have found something interesting about ARA promoter。Besides,we have also found that we can use E.coli itself to hydrolyze pseudo-ginseng ,and it is safer than E.coli with plasmid we designed. And we think it fits our theme―food and nutation.









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