|
|
| Line 63: |
Line 63: |
| | <div class="page-title"><h5>Circuit Design</h5> | | <div class="page-title"><h5>Circuit Design</h5> |
| | </div> <br /><br /><br /><br /><br /><br /> | | </div> <br /><br /><br /><br /><br /><br /> |
| − | <section><h3>The quest began with the search for ways to curb the problem to capture as well as detection of noxious gases and harmful chemicals. The task was to make a circuit with feedback positive and negative loops for increasing the sensitivity and specificity of detection. We came up with a novel circuit that can be modified easily for detection of various pollutants. Some pollutants activate a promoter whereas others repress a promoter. For eg: CO activates the COOA promoter whereas Acetaldehyde represses XylR promoter. The circuit we have designed can be used for both kinds of pollutants</h3> | + | <section><h3>The quest began with the search for ways to curb the problem of capturing and detecting noxious gases and harmful chemicals. The task was to make a circuit with feedback positive and negative loops for increasing the sensitivity and specificity of detection. We came up with a novel circuit that can be modified easily for detecting various pollutants. Some pollutants activate, whereas others repress, a promoter. For eg. CO activates the COOA promoter whereas Acetaldehyde represses the XylR promoter. The circuit we have designed can be used for both kinds of pollutants.</h3> |
| | </section> | | </section> |
| | <section><br /><br /><br /><br /> | | <section><br /><br /><br /><br /> |
| − | <h3>Circuit 1 represents the circuit for activator whereas Circuit 2 represents circuit for repressor type pollutant.</h3> | + | <h3>Circuit 1 and Circuit 2 represent circuits for activator and repressor type pollutants respectively.</h3> |
| | <center><div class="data-img"><img width="80%" src="https://static.igem.org/mediawiki/2017/9/99/T--IISER-Mohali-INDIA--circuit2.png" alt="Salicylate"></div> | | <center><div class="data-img"><img width="80%" src="https://static.igem.org/mediawiki/2017/9/99/T--IISER-Mohali-INDIA--circuit2.png" alt="Salicylate"></div> |
| | <h4> Ciruit 1 <h4/> | | <h4> Ciruit 1 <h4/> |
| − | <h4> Different pollutants can be used with their activating circuits having respective promoters to replace module 3 appropriately. Examples of pollutants acting as positive regulators include CO, Xylene, NO etc.</h4><br/><br/> | + | <h4> Different pollutants with their activating circuits and respective promoters can be used to replace module 3 accordingly. Examples of pollutants acting as positive regulators include CO, Xylene, NO etc.</h4><br/><br/> |
| | <div src="data-img"> | | <div src="data-img"> |
| | <img src="https://static.igem.org/mediawiki/2017/c/cc/T--IISER-Mohali-INDIA--circuit1.png" alt="Acetaldehyde" width="80%"> | | <img src="https://static.igem.org/mediawiki/2017/c/cc/T--IISER-Mohali-INDIA--circuit1.png" alt="Acetaldehyde" width="80%"> |
| | | | |
| | <h4> Ciruit 2 <h4/> | | <h4> Ciruit 2 <h4/> |
| − | <h4> Different pollutants that can negatively regulate respective promoters can replace first part of module 3 appropriately. <h4/><br/> | + | <h4> Different pollutants that can negatively regulate their respective promoters can replace the first part of module 3 accordingly.<h4/><br/> |
| | </center> | | </center> |
| | </section> | | </section> |
| | <br /> | | <br /> |
| | <section> | | <section> |
| − | <h3>Circuit can be divided into 3 parts. The bottom circuit gives a color when there is no pollutant. It | + | <h3>The circuit can be divided into 3 parts. The bottom circuit gives a color when there is no pollutant. It |
| − | signifies the number of cells alive and with an ability to form chromoprotein. In the presence of | + | signifies the number of cells alive and and capable of forming chromoprotein. In the presence of a |
| − | pollutant, the topmost part detects the pollutant and activates the formation of second | + | pollutant, the topmost part detects the pollutant and activates the formation of a second |
| − | chromoprotein in the middle circuit. Simultaneously, it inhibits the production of first | + | chromoprotein in the middle circuit. Simultaneously, it inhibits the production of the first |
| | chromoprotein. This results in the differential color formation depending upon the concentration of | | chromoprotein. This results in the differential color formation depending upon the concentration of |
| − | pollutant [1]. It has been documented that positive feedback has a stabilising effect on circuit. Also, | + | pollutant [1]. It has been documented that positive feedbacks have stabilizing effects on circuits. Also, |
| − | negative feedback loops makes a system robust against alterations and noise [1].</h3> | + | negative feedback loops make systems robust against alterations and noise [1].</h3> |
| | </section> | | </section> |
| | | | |
