Difference between revisions of "Team:IIT Delhi/recipe"

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            <h2> 20x TAE buffer </h2>
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            20x TAE buffer
 
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           <li>80mL of 1M Tris solution
 
           <li>80mL of 1M Tris solution
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           <h2 id="pfont"> For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O.
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           <ul>For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O.
 
Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap
 
Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap
 
the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and
 
the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and
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store at room temperature. </h2>
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store at room temperature. </ul>
 
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Revision as of 20:28, 31 October 2017

iGEM IIT Delhi

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RECIPES

                                                                                                                                                                                                                 

Accurate Experimenting for Accurate Results !!

20x TAE buffer
  • 80mL of 1M Tris solution
  • 0.7264g of EDTANa2 salt
  • 2.284 mL glacial acetic acid
  • distilled H2O 100mL
    For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O. Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and store at room temperature.
  • 50 mM glucose.
  • 25 mM Tris-Cl (pH 8.0).
  • 10 mM EDTA (pH 8.0).
Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by autoclaving and store at 4°C.
0.2 N NaOH (freshly diluted from a 10 N stock).(100ulsds + 700ul mq + 200ul naoh) 1% (w/v) SDS.
  • 5 M potassium acetate, 60.0 ml
  • Glacial acetic acid, 11.5 ml
  • H2O, 28.5 ml
The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate. Store the solution at 4°C and transfer it to an ice bucket just before use.

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