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     <h3 id="FecalSamples" style="color:#674A8A;"> High-Throughput Sequencing </h3>
 
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     <p>Concentration protocol to concentrate DNA isolating using the PowerFecal Extraction kit.<p>
 
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     <p>Illumina MiSeq Sequencing was used to identify the sequences of the 16S rRNA genes in the fecal samples. This step was undertaking by the Integrated Microbiome Resource at Dal. There protocol was provided to us.<p>
 
     <p>Illumina MiSeq Sequencing was used to identify the sequences of the 16S rRNA genes in the fecal samples. This step was undertaking by the Integrated Microbiome Resource at Dal. There protocol was provided to us.<p>
 
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Revision as of 00:05, 1 November 2017

Experiments

High-Throughput Sequencing

Fecal Sample DNA Extraction

To extract environmental DNA from fecal samples. This extraction will purify genomic DNA from prokaryotic and eukaryotic cells found in the fecal sample.

DNA Concentration

Concentration protocol to concentrate DNA isolating using the PowerFecal Extraction kit.

16S Illumina Miseq Sequencing

Illumina MiSeq Sequencing was used to identify the sequences of the 16S rRNA genes in the fecal samples. This step was undertaking by the Integrated Microbiome Resource at Dal. There protocol was provided to us.

Metagenomic Shotgun Illumina Miseq Sequencing

This protocol was used by the Integrated Microbiome Resource to sequence the metagenomic DNA in our fecal samples from the porcupine, beaver, arctic wolf and coyote.

16S Sequencing Analysis

This protocol was used for analyzing the 16S Illumina Sequencing data from the illumina sequencer. Provided by the Integrated Microbiome Resource at Dalhousie University.