Difference between revisions of "Team:Shanghaitech/Experiment and Modeling"
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<h2>Orthogonality test</h2> | <h2>Orthogonality test</h2> | ||
| − | Applying multiple quorumsensing systems in single bacteria requires low crosstalk activities between AHLs and LuxR-like proteins. We've tested signal molecule orthogonality in several Fluorescent AHL Receivers we construct. | + | Applying multiple quorumsensing systems in single bacteria requires low crosstalk activities between AHLs and LuxR-like proteins. We've tested signal molecule orthogonality in several Fluorescent AHL Receivers we construct.</p> |
| − | <img src='https://static.igem.org/mediawiki/2017/2/23/T--Shanghaitech--LasRorthogonality1.png' | + | <center><img src='https://static.igem.org/mediawiki/2017/2/23/T--Shanghaitech--LasRorthogonality1.png' /><center> |
| + | <p><b>Fig. 1 Orthogonality test of LasR-pLas-GFP </b> (i):Fluorescent response to cognate and non-cognate AHLs (ii)Dose-Response curves for cognate and non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with 3OC12-HSL</p> | ||
| + | <center><img src='https://static.igem.org/mediawiki/2017/0/04/T--Shanghaitech--rparortho1fx.png' /><center> | ||
Revision as of 22:47, 1 November 2017
Experiment and Modeling
Testing Fluorescent AHL Receivers
Orthogonality test
Applying multiple quorumsensing systems in single bacteria requires low crosstalk activities between AHLs and LuxR-like proteins. We've tested signal molecule orthogonality in several Fluorescent AHL Receivers we construct.
Fig. 1 Orthogonality test of LasR-pLas-GFP (i):Fluorescent response to cognate and non-cognate AHLs (ii)Dose-Response curves for cognate and non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with 3OC12-HSL
