Difference between revisions of "Team:BGIC-Union/InterLab"

Line 1: Line 1:
 +
 
<html>
 
<html>
 +
  <head>
 
     <meta charset="utf-8">
 
     <meta charset="utf-8">
 
    
 
    
Line 5: Line 7:
  
  
−
     <title>Safety</title>
+
     <title>Team：BGIC-Union</title>
  
 
   <head>
 
   <head>
 
    
 
    
−
        <link href='http://fonts.googleapis.com/css?family=Lato:400,300' rel='stylesheet' type='text/css'>
+
<!-- main -->
−
        <link href='http://fonts.googleapis.com/css?family=Raleway:400,300,500' rel='stylesheet' type='text/css'>
+
−
 
+
−
  <!-- Bootstrap core CSS -->
+
−
    <link href="https://2017.igem.org/Template:BGIC-Union/bootstrap-css?action=raw&amp;ctype=text/css" rel="stylesheet">
+
−
<!-- animate -->
+
−
<link href="https://2017.igem.org/Template:BGIC-Union/animate-css?action=raw&amp;ctype=text/css" rel="stylesheet">
+
−
  <!-- carousel -->
+
−
  <link href="https://2017.igem.org/Template:BGIC-Union/owlcarousel-css?action=raw&amp;ctype=text/css" rel="stylesheet">
+
−
  <link href=" https://2017.igem.org/Template:BGIC-Union/fontawesomemin2-css?action=raw&amp;ctype=text/css" rel="stylesheet">
+
−
 
+
−
 
+
−
  <!-- main -->
+
 
   <style type="text/css">
 
   <style type="text/css">
 
    
 
    
Line 34: Line 24:
  
  
−
 
+
#footer{
 +
padding-botton:-60px;
 +
}
  
 
#content{
 
#content{
Line 45: Line 37:
 
     margin: 0 !important;
 
     margin: 0 !important;
 
     padding: 0 !important;
 
     padding: 0 !important;
 +
 
}
 
}
 
body {
 
body {
Line 50: Line 43:
 
   font-family: 'Montserrat', sans-serif;
 
   font-family: 'Montserrat', sans-serif;
 
     font-weight: 400;
 
     font-weight: 400;
−
     font-size: 14px;
+
line-height: 45px;
 +
     font-size: 20px;
 
     color: #555;
 
     color: #555;
−
     ；
+
 
 +
      
 
     -webkit-font-smoothing: antialiased;
 
     -webkit-font-smoothing: antialiased;
 
     -webkit-overflow-scrolling: touch;
 
     -webkit-overflow-scrolling: touch;
Line 58: Line 53:
  
 
/* Titles */
 
/* Titles */
−
h1, h2, h3, h4, h5, h6 {
+
h1, h2, h3, h4, h5, h6,p {
 
font-family: 'Montserrat', sans-serif;
 
font-family: 'Montserrat', sans-serif;
−
     font-weight: 700;
+
     font-weight: 600;
 
     color: #333;
 
     color: #333;
 +
line-height:30pt;
 
}
 
}
  
Line 67: Line 63:
 
font-size: 35px;
 
font-size: 35px;
 
margin-top: 30px;
 
margin-top: 30px;
−
margin-bottom: 30px;
+
margin-bottom: 50px;
 
}
 
}
  
  
  
−
/* Paragraph &amp; Typographic */
+
/* Paragraph Typographic */
 
p {
 
p {
−
     line-height: 28px;
+
     line-height: 50px;
−
     margin-bottom: 25px;
+
     margin-bottom: 35px;
 
     font-size: 16px;
 
     font-size: 16px;
 
}
 
}
Line 120: Line 116:
 
}
 
}
  
−
.copyrights{
 
−
text-indent:-9999px;
 
−
height:0;
 
−
line-height:0;
 
−
font-size:0;
 
−
overflow:hidden;
 
−
}
 
  
 
.navbar {
 
.navbar {
Line 133: Line 122:
 
}
 
}
  
 +
top1 {
 +
padding-top: -20px;
 +
]
 
.navbar-inverse {
 
.navbar-inverse {
 
padding-bottom: 70px;
 
padding-bottom: 70px;
Line 181: Line 173:
 
padding-bottom: 50px;
 
padding-bottom: 50px;
 
}
 
}
−
 
+
.lr {
 +
        PADDING-LEFT: 100PX;
 +
        padding-right: 100px;
 +
}
  
 
/* +++++ WRAP SECTIONS +++++ */
 
/* +++++ WRAP SECTIONS +++++ */
Line 285: Line 280:
 
}
 
}
 
   </style>
 
   </style>
 +
 
    
 
    
 +
  <!-- Bootstrap core CSS -->
 +
    <link href="https://2017.igem.org/Template:BGIC-Union/bootstrap-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
<!-- animate -->
 +
<link href="https://2017.igem.org/Template:BGIC-Union/animate-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
  <!-- carousel -->
 +
  <link href="https://2017.igem.org/Template:BGIC-Union/owlcarousel-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
  <link href=" https://2017.igem.org/Template:BGIC-Union/fontawesomemin2-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
 +
  <!-- main -->
 +
    <link href="https://2017.igem.org/Team:BGIC-Union/finalcss/main-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
  <!-- slider -->
 +
  <style type="text/css">
 +
 +
  /* Section Slider
 +
========================================================= */
 +
 +
/* Carousel Slider
 +
============================================== */
 +
#slider_part {
 +
    height: 900px;
 +
    width: 100%;
 +
    overflow: hidden;
 +
background-color: #f2f2f2;
 +
}
 +
 +
.carousel-caption {
 +
    top: 30%;
 +
    margin-top: -70px;
 +
    text-shadow: none!important;
 +
    left: 9%;
 +
    right: 10%;
 +
    -webkit-transition: all.3s ease 0s;
 +
    -o-transition: all.3s ease 0s;
 +
    transition: all.3s ease 0s;
 +
    text-align: center;
 +
}
 +
 +
.carousel-caption h3 {
 +
    text-shadow: none;
 +
    font-size: 70px;
 +
    line-height: 92px;
 +
    font-weight: 300;
 +
    margin: 0 0px;
 +
    transition-delay: .3s;
 +
    -webkit-transition-delay: .3s;
 +
    -o-transition-delay: .3s;
 +
    text-transform: capitalize!important;
 +
}
 +
 +
.carousel-caption p {
 +
    font-size: 46px;
 +
    line-height: 62px;
 +
    font-weight: 500;
 +
    color: #fff;
 +
    transition-delay: .3s;
 +
    -moz-transition-delay: .3s;
 +
    -o-transition-delay: .3s;
 +
    margin: 40px 0px;
 +
    text-transform: uppercase;
 +
font-family: Comic Sans MS;
 +
}
 +
 +
.carousel-caption img {
 +
    border: 2px solid#fff;
 +
    padding: 10px 10px 10px 20px;
 +
}
 +
 +
.line {
 +
    width: 100px;
 +
    height: 5px;
 +
    color: #222;
 +
    margin: 0px auto;
 +
}
 +
 +
.carousel-caption p i {
 +
    font-size: 7px;
 +
    margin: 0px 3px;
 +
    position: relative;
 +
    top: -3px;
 +
}
 +
 +
.carousel .item {
 +
    -webkit-transition: opacity .3s;
 +
    -moz-transition: opacity .3s;
 +
    -ms-transition: opacity .3s;
 +
    -o-transition: opacity .3s;
 +
    transition: opacity .3s;
 +
}
 +
 +
.btn-featured {
 +
    text-decoration: none;
 +
    position: relative;
 +
    -webkit-transition: all .3s ease-in-out;
 +
    -o-transition: all .3s ease-in-out;
 +
    transition: all .3s ease-in-out;
 +
    background: transparent;
 +
    border-radius: 2px;
 +
    z-index: 1;
 +
    font-weight: 500;
 +
    font-size: 18px;
 +
    line-height: 20px;
 +
    padding: 15px 40px !important;
 +
    text-transform: uppercase;
 +
    margin-top: 20px;
 +
    margin-right: 10px;
 +
}
 +
 +
.carousel-control.left,.carousel-control.right {
 +
    background-image: none !important;
 +
    background-repeat: no-repeat !important;
 +
}
 +
 +
.carousel-control {
 +
    width: 4% !important;
 +
}
 +
 +
.carousel-caption img {
 +
    height: auto;
 +
}
 +
 +
.carousel-indicators {
 +
    bottom: 100px;
 +
    width: 10%;
 +
    left: 45%;
 +
    margin: 0 auto;
 +
    bottom: 30%;
 +
}
 +
 +
.carousel-indicators li {
 +
    width: 20px !important;
 +
    height: 20px !important;
 +
    border: 2px solid #FFF !important;
 +
    margin: 0px !important;
 +
    border-radius: 50%;
 +
}
 +
 +
.carousel-control.left span {
 +
    padding: 15px;
 +
}
 +
 +
.slides-control .carousel-control i {
 +
    line-height: 36px;
 +
    font-size: 32px;
 +
    padding-top: 6px;
 +
    -moz-transition: all 500ms ease;
 +
    -webkit-transition: all 500ms ease;
 +
    -ms-transition: all 500ms ease;
 +
    -o-transition: all 500ms ease;
 +
    transition: all 500ms ease;
 +
    border-radius: 50%;
 +
    width: 50px;
 +
    height: 50px;
 +
}
 +
 +
.slides-control .carousel-control {
 +
    position: absolute;
 +
    width: 240px;
 +
    top: 45%;
 +
    z-index: 10;
 +
}
 +
 +
.carousel-control.left {
 +
    left: -15px;
 +
}
 +
 +
.carousel-control.right {
 +
    right: 0px;
 +
}
 +
 +
.carousel-control.left {
 +
    width: 120px;
 +
    height: 40px;
 +
}
 +
 +
.carousel-control.right {
 +
    width: 120px;
 +
    height: 40px;
 +
}
 +
 +
.overlay-slide {
 +
    background: #000;
 +
    height: 65%;
 +
    width: 100%;
 +
}
 +
 +
.overlay-slide img {
 +
    opacity: 1;
 +
    width: 100%;
 +
    height: auto;
 +
}
 +
 +
/*-- Animation --*/
 +
 +
.carousel .item.active .animated2 {
 +
    -webkit-animation: lightSpeedIn 1s ease-in 200ms both;
 +
    animation: lightSpeedIn 1s ease-in 200ms both;
 +
}
 +
 +
.carousel .item.active .animated3 .animated4 {
 +
    -webkit-animation: bounceInLeft 1s ease-in-out 500ms both;
 +
    animation: bounceInLeft 1s ease-in-out 500ms both;
 +
}
 +
.animated4 {color:#4169E1
 +
}
 +
.img{
 +
-webkit-filter: brightness(1);
 +
}
 +
 +
  </style>
 +
  <!-- script -->
 +
  <script src="https://code.jquery.com/jquery-1.10.2.min.js"></script>
 +
  <script type="text/javascript">
 +
  /*
 +
* jQuery Easing v1.3 - http://gsgd.co.uk/sandbox/jquery/easing/
 +
*
 +
* Uses the built in easing capabilities added In jQuery 1.1
 +
* to offer multiple easing options
 +
*
 +
* TERMS OF USE - jQuery Easing
 +
*
 +
* Open source under the BSD License.
 +
*
 +
* Copyright © 2008 George McGinley Smith
 +
* All rights reserved.
 +
*
 +
* Redistribution and use in source and binary forms, with or without modification,
 +
* are permitted provided that the following conditions are met:
 +
*
 +
* Redistributions of source code must retain the above copyright notice, this list of
 +
* conditions and the following disclaimer.
 +
* Redistributions in binary form must reproduce the above copyright notice, this list
 +
* of conditions and the following disclaimer in the documentation and/or other materials
 +
* provided with the distribution.
 +
*
 +
* Neither the name of the author nor the names of contributors may be used to endorse
 +
* or promote products derived from this software without specific prior written permission.
 +
*
 +
* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
 +
* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
 +
* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
 +
*  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
 +
*  EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE
 +
*  GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED
 +
* AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING
 +
*  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
 +
* OF THE POSSIBILITY OF SUCH DAMAGE.
 +
*
 +
*/
 +
 +
// t: current time, b: begInnIng value, c: change In value, d: duration
 +
jQuery.easing['jswing'] = jQuery.easing['swing'];
 +
 +
jQuery.extend( jQuery.easing,
 +
{
 +
def: 'easeOutQuad',
 +
swing: function (x, t, b, c, d) {
 +
//alert(jQuery.easing.default);
 +
return jQuery.easing[jQuery.easing.def](x, t, b, c, d);
 +
},
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easeInQuad: function (x, t, b, c, d) {
 +
return c*(t/=d)*t + b;
 +
},
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easeOutQuad: function (x, t, b, c, d) {
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return -c *(t/=d)*(t-2) + b;
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},
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easeInOutQuad: function (x, t, b, c, d) {
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if ((t/=d/2) < 1) return c/2*t*t + b;
 +
return -c/2 * ((--t)*(t-2) - 1) + b;
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},
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easeInCubic: function (x, t, b, c, d) {
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return c*(t/=d)*t*t + b;
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},
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easeOutCubic: function (x, t, b, c, d) {
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return c*((t=t/d-1)*t*t + 1) + b;
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},
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easeInOutCubic: function (x, t, b, c, d) {
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if ((t/=d/2) < 1) return c/2*t*t*t + b;
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return c/2*((t-=2)*t*t + 2) + b;
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},
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easeInQuart: function (x, t, b, c, d) {
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return c*(t/=d)*t*t*t + b;
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},
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easeOutQuart: function (x, t, b, c, d) {
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return -c * ((t=t/d-1)*t*t*t - 1) + b;
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easeInOutQuart: function (x, t, b, c, d) {
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if ((t/=d/2) < 1) return c/2*t*t*t*t + b;
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return -c/2 * ((t-=2)*t*t*t - 2) + b;
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},
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easeInQuint: function (x, t, b, c, d) {
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return c*(t/=d)*t*t*t*t + b;
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},
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easeOutQuint: function (x, t, b, c, d) {
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return c*((t=t/d-1)*t*t*t*t + 1) + b;
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},
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easeInOutQuint: function (x, t, b, c, d) {
 +
if ((t/=d/2) < 1) return c/2*t*t*t*t*t + b;
 +
return c/2*((t-=2)*t*t*t*t + 2) + b;
 +
},
 +
easeInSine: function (x, t, b, c, d) {
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return -c * Math.cos(t/d * (Math.PI/2)) + c + b;
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},
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easeOutSine: function (x, t, b, c, d) {
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return c * Math.sin(t/d * (Math.PI/2)) + b;
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},
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easeInOutSine: function (x, t, b, c, d) {
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return -c/2 * (Math.cos(Math.PI*t/d) - 1) + b;
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},
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easeInExpo: function (x, t, b, c, d) {
 +
return (t==0) ? b : c * Math.pow(2, 10 * (t/d - 1)) + b;
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},
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easeOutExpo: function (x, t, b, c, d) {
 +
return (t==d) ? b+c : c * (-Math.pow(2, -10 * t/d) + 1) + b;
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},
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easeInOutExpo: function (x, t, b, c, d) {
 +
if (t==0) return b;
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if (t==d) return b+c;
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if ((t/=d/2) < 1) return c/2 * Math.pow(2, 10 * (t - 1)) + b;
 +
return c/2 * (-Math.pow(2, -10 * --t) + 2) + b;
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},
 +
easeInCirc: function (x, t, b, c, d) {
 +
return -c * (Math.sqrt(1 - (t/=d)*t) - 1) + b;
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},
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easeOutCirc: function (x, t, b, c, d) {
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return c * Math.sqrt(1 - (t=t/d-1)*t) + b;
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},
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easeInOutCirc: function (x, t, b, c, d) {
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if ((t/=d/2) < 1) return -c/2 * (Math.sqrt(1 - t*t) - 1) + b;
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return c/2 * (Math.sqrt(1 - (t-=2)*t) + 1) + b;
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},
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easeInElastic: function (x, t, b, c, d) {
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var s=1.70158;var p=0;var a=c;
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if (t==0) return b;  if ((t/=d)==1) return b+c;  if (!p) p=d*.3;
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if (a < Math.abs(c)) { a=c; var s=p/4; }
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else var s = p/(2*Math.PI) * Math.asin (c/a);
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return -(a*Math.pow(2,10*(t-=1)) * Math.sin( (t*d-s)*(2*Math.PI)/p )) + b;
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},
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easeOutElastic: function (x, t, b, c, d) {
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var s=1.70158;var p=0;var a=c;
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if (t==0) return b;  if ((t/=d)==1) return b+c;  if (!p) p=d*.3;
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if (a < Math.abs(c)) { a=c; var s=p/4; }
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else var s = p/(2*Math.PI) * Math.asin (c/a);
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return a*Math.pow(2,-10*t) * Math.sin( (t*d-s)*(2*Math.PI)/p ) + c + b;
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},
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easeInOutElastic: function (x, t, b, c, d) {
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var s=1.70158;var p=0;var a=c;
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if (t==0) return b;  if ((t/=d/2)==2) return b+c;  if (!p) p=d*(.3*1.5);
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if (a < Math.abs(c)) { a=c; var s=p/4; }
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else var s = p/(2*Math.PI) * Math.asin (c/a);
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if (t < 1) return -.5*(a*Math.pow(2,10*(t-=1)) * Math.sin( (t*d-s)*(2*Math.PI)/p )) + b;
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return a*Math.pow(2,-10*(t-=1)) * Math.sin( (t*d-s)*(2*Math.PI)/p )*.5 + c + b;
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},
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easeInBack: function (x, t, b, c, d, s) {
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if (s == undefined) s = 1.70158;
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return c*(t/=d)*t*((s+1)*t - s) + b;
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},
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easeOutBack: function (x, t, b, c, d, s) {
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if (s == undefined) s = 1.70158;
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return c*((t=t/d-1)*t*((s+1)*t + s) + 1) + b;
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},
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easeInOutBack: function (x, t, b, c, d, s) {
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if (s == undefined) s = 1.70158;
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if ((t/=d/2) < 1) return c/2*(t*t*(((s*=(1.525))+1)*t - s)) + b;
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return c/2*((t-=2)*t*(((s*=(1.525))+1)*t + s) + 2) + b;
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},
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easeInBounce: function (x, t, b, c, d) {
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return c - jQuery.easing.easeOutBounce (x, d-t, 0, c, d) + b;
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},
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easeOutBounce: function (x, t, b, c, d) {
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if ((t/=d) < (1/2.75)) {
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return c*(7.5625*t*t) + b;
 +
} else if (t < (2/2.75)) {
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return c*(7.5625*(t-=(1.5/2.75))*t + .75) + b;
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} else if (t < (2.5/2.75)) {
 +
return c*(7.5625*(t-=(2.25/2.75))*t + .9375) + b;
 +
} else {
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return c*(7.5625*(t-=(2.625/2.75))*t + .984375) + b;
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}
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},
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easeInOutBounce: function (x, t, b, c, d) {
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if (t < d/2) return jQuery.easing.easeInBounce (x, t*2, 0, c, d) * .5 + b;
 +
return jQuery.easing.easeOutBounce (x, t*2-d, 0, c, d) * .5 + c*.5 + b;
 +
}
 +
});
 +
 +
/*
 +
*
 +
* TERMS OF USE - EASING EQUATIONS
 +
*
 +
* Open source under the BSD License.
 +
*
 +
* Copyright © 2001 Robert Penner
 +
* All rights reserved.
 +
*
 +
* Redistribution and use in source and binary forms, with or without modification,
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Revision as of 03:02, 1 November 2017

Team：BGIC-Union




We are excited to have a chance to participate in the Fourth International Interlaboratory Study as a team in IGEM, 2017 and contribute our data as part of the GFP measurement. Interlab study requires us to transform eight plasmids into DH5α E.coli cell in order to measure OD 600 and Fluorescence of them as the time passes by.

Calibration:


1.Mea sure the Abs600 of both the LUDOX-S40 and water to obtain the correction factor.
2.Measure the fluorescence of a series of diluted fluorescein to draw the fluorescein standard curve.

Cell Measurement Procedure:



1. At first, we transform eight plasmids into DH5α E. Coli cell which include Positive control, Negative Control, D1-D6. After 12 hours, we pick one colony from each plate and inoculate each into a 50ml Falcon tube.

2. After a 12 hours incubation, we measure the primary OD600 and dilute bacteria solution into an OD600 of 0.02.

3. Finally, we measure the OD600 and Fluorescein every 2 hours and record the data into table.

Observations



1. It takes us much time to dilute the bacteria solution to the OD600 of 0.02. So the exact value of the concentration of bacteria solution may be incorrect.
2. As a team composed of high school students, sometimes we feel confused in understanding the procedures and the theory behind the instructions.
3. Since our data is very different compared to other teams’, maybe there are some improper operations or other mistakes in our study.


Finally, we sincerely appreciate BGI for permitting us to use the plate reader.