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Ethylene glycol and Terephthalic acid are used as energy source for I. sakaiensis. | Ethylene glycol and Terephthalic acid are used as energy source for I. sakaiensis. | ||
+ | </article> | ||
+ | <article> | ||
+ | We considered using the I. sakaiensis bacteria itself, but we decided that working with a newly discovered and relatively undocumented bacteria was too risky. So we had to find a safe and effective way of recreating something with the abilities of I. sakaiensis. | ||
+ | </article> | ||
+ | <article> | ||
+ | Instead of using the Ideonella sakaiensis bacteria to degrade plastic, we decided to insert the genes for plastic degradation into K-12 E.coli bacteria. We chose K-12 E. coli because it is safe to use, easily grown in the lab, and very well documented since it is commonly used. | ||
+ | </article> | ||
+ | <h4>Our Constructs</h4> | ||
+ | <article> | ||
+ | After obtaining the E.coli, we designed a construct, known as a biobrick, to allow the bacteria to express the proteins lipase and esterase that will degrade PET plastics. | ||
+ | </article> | ||
+ | <article> | ||
+ | First is the Lac Regulated Promoter, which starts transcription in the presence of lactose or IPTG. | ||
+ | </article> | ||
+ | <article> | ||
+ | Secondly, we had the Ribosome Binding site which signals the start of translation from mRNA to protein. | ||
</article> | </article> | ||
</section> | </section> |
Revision as of 03:58, 1 November 2017
BALTIMORE BIO-CREW
Bio-Engineering E.Coli To Degrade Plastic and Save The Baltimore Inner Harbor
Design
- catalyzes PET hydrolysis
- Turns PET into MHET (Mono (2-hydroxyethyl) Terephthalate acid)
- Breaks down MHET
- Turns MHET into ethylene glycol and terephthalic acid.