Revision as of 05:26, 1 November 2017

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Results

Fractionation controls and protein expression

Figures 1-3 depict our fractionation controls and blot for the identification of saCas9 protein (expected size ~130 kB). The rightmost column in the blot, labelled "control" is a purified His-tagged protein and was used to ensure that the anti-His antibody could properly bind the protein in question. The fractionation results indicated that although Maltose-binding periplasmic protein (MBP) was exclusively present in the periplasmic fraction, showing strong bands of the expected size (42.5kDa), GroEl was visible in both the cell's periplasm and cytoplasm suggesting leakage from the cytoplasm to the periplasm.

Cas9 functionality assay

OMV visualization

Vesicle delivery to Top10 cells - TEM visualization

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 <h3><br><br><center>Fractionation controls and protein expression</h3></center>
+<p style="padding-top:2%; padding-right: 15%; padding-left:15%; font-size:14px;" class="big">Figures 1-3 depict our fractionation controls and blot for the identification of saCas9 protein (expected size ~130 kB). The rightmost column in the blot, labelled "control" is a purified His-tagged protein and was used to ensure that the anti-His antibody could properly bind the protein in question. The fractionation results indicated that although Maltose-binding periplasmic protein (MBP) was exclusively present in the periplasmic fraction, showing strong bands of the expected size (42.5kDa), GroEl was visible in both the cell's periplasm and cytoplasm suggesting leakage from the cytoplasm to the periplasm. </p>
 </div>
 <p style="padding-top:2%; padding-right: 15%; padding-left:15%; font-size:14px;" class="big">