Difference between revisions of "Team:TCFSH Taiwan/Results"

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     <p class="content-1">Chromoprotein o.d. value</p>
 
     <p class="content-1">Chromoprotein o.d. value</p>
−
     <p class="content">(1)  RFP (E1010): 607nm<br>(2) BFP (k592009):588nm</br><br>(3) cjBlue (k592011):610nm</br></p>
+
     <p class="content"><br>(1)  RFP (E1010): 607nm</br><br>(2) BFP (k592009):588nm</br><br>(3) cjBlue (k592011):610nm</br></p>
  
 
     <p class="content-1">Culture Setting</p>
 
     <p class="content-1">Culture Setting</p>
−
     <p class="content">(1)  32ﾟC, 200rpm, culture12hr (15ml) *continue to log phase<br>(2) add (15ml) LB *re-culture</br><br>(3) TeﾟC, continue cultural</br><br>(4) Take samples from the centrifuge tube per hour, and narrow the time range and improve the accuracy.</br></p>
+
     <p class="content"><br>(1)  32ﾟC, 200rpm, culture12hr (15ml) *continue to log phase</br><br>(2) add (15ml) LB *re-culture</br><br>(3) TeﾟC, continue cultural</br><br>(4) Take samples from the centrifuge tube per hour, and narrow the time range and improve the accuracy.</br></p>
  
  

Revision as of 06:39, 1 November 2017

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JUDGING FORM

Experiment

Our product aims to reach a high quality of “long-term monitor”, which means considerably stable expression of color is needed. Thus, we choose chromoprotein as our reporter protein due to its steadily expression and low half-life degradation rate. (See more in here)

cj-Blue

We add a LVA tag to cjBlue in order to accelerate its degrade speed. (See more in here)

Concentration simulation of cjBlue

Chromoprotein

According to our model of chromoprotein

Expression

Temperature

35ﾟC / 37ﾟC / 39ﾟC / 32ﾟC

Chromoprotein o.d. value


(1) RFP (E1010): 607nm

(2) BFP (k592009):588nm

(3) cjBlue (k592011):610nm

Culture Setting


(1) 32ﾟC, 200rpm, culture12hr (15ml) *continue to log phase

(2) add (15ml) LB *re-culture

(3) TeﾟC, continue cultural

(4) Take samples from the centrifuge tube per hour, and narrow the time range and improve the accuracy.